Carolyn Ruth Bertozzi

The paper maps age-related changes in the N-glycoproteome across multiple tissues in mice, showing how glycosylation affects autoantibody binding to MOG in the brain.

This study maps the human cell surface proteome at nanoscale resolution, identifying functional modules and spatial organization patterns in T-lymphocytes and B-lymphoblasts.

This paper develops GELYTACs, lysosome-targeting chimeras that deplete IgG in vivo via AAV delivery, showing sustained antibody depletion in mice.

The paper develops TICTACs, which selectively target and degrade checkpoint receptors on tumor-associated macrophages without affecting other cells.

The paper develops a bioorthogonal reporter for MGAT1 by iteratively engineering a UDP-GlcNAc analog with a chemical tag, enabling specific substrate incorporation in mammalian cells.

The paper introduces GlycoShuttles, mucin-binding protein shuttles that deliver therapeutics across the blood-brain barrier into brain cells, showing efficacy in dementia models.

The paper uses thermal proteome profiling to study how glycosylation affects protein function and stability, finding that sialylation and fucosylation perturbations significantly impact cell surface protein interactions.

This paper introduces a color-neutral and reversible tissue transparency method that enables longitudinal deep-tissue imaging in live mice with high spatiotemporal resolution.

The paper introduces irCLIP-RNP and Re-CLIP methods to identify dynamic RNA-protein assemblies, revealing new patterns of RBP-RNA associations and EGF-induced remodeling.

This study characterizes transcriptomic and metabolomic profiles in human urine, identifying distinct cell type repertoires and metabolic signals compared to plasma.

The paper identifies acp3U as the site where N-glycans attach to RNA in glycoRNA using rPAL and SWATH-MS.

The paper describes the development of genetically encodable LYTACs (GELYTACs) for targeted protein degradation using engineered T-cells, showing efficacy in secreting and delivering the therapeutics.

The paper develops a method to image phenolic glycolipid (PGL) in mycobacteria using bioorthogonal metabolic labeling and demonstrates its application in studying PGL dynamics.

The paper identifies sortilin as the receptor mediating galectin-1 lysosomal trafficking through a CRISPR screen and demonstrates its use for siRNA delivery.

The paper designs EndoTags to trigger endocytosis and degradation of target proteins, enabling tissue-specific targeting and therapeutic applications.

This study maps the organism-wide secretome response to exercise in mice, identifying 200+ exercise-regulated protein pairs and uncovering anti-obesity and anti-diabetic roles of a liver-secreted proteoform.

The paper introduces BOCTAG, a method to label glycoproteins in specific cell types within mixed cultures, enabling analysis of cell-specific glycosylation.

The paper uses CRISPR screens to identify mucins as key host factors that restrict SARS-CoV-2 infection in human lung cells.

Hierarch is a Python package that performs hypothesis tests and computes confidence intervals for hierarchical experimental designs using resampling methods, with fast computation via Numba.

O-Pair Search identifies O-glycopeptides and localizes O-glycosites using collision- and electron-based dissociation spectra, improving speed and identification rates.

This paper introduces GalNAzMe, a monosaccharide probe that selectively labels O-GalNAc glycosylation in living cells, enabling detailed study of this cancer-relevant modification.

The paper introduces a method to chemically tag glycans in living cells using engineered glycosyltransferases to study glycan structure and biosynthesis.

The paper develops a voltage imaging platform to study how glycans affect neuronal connectivity and shows specific glycan ablations impact functional connectivity in mouse hippocampal neurons.

The paper identifies lipid droplet-accumulating microglia (LAM) as a dysfunctional, pro-inflammatory state in aging brains, linked to neurodegeneration.

The paper identifies N-glycan modifications on mammalian Y RNAs, showing RNA can be a scaffold for glycosylation in the secretory pathway.