Antibiotic Resistance in Bacteria

This study examines β-lactam-resistant E. coli in calves across Wallonia, Belgium, finding that antibiotic restrictions reduced resistance but not population structure, with evidence of indirect farm transmission and potential human adaptation.

The paper identifies a clinically silent resistance phenotype in Acinetobacter baumannii that allows survival during colistin therapy despite standard susceptibility testing.

This study uses historical isolates to track the evolution of Acinetobacter baumannii international clone 2 and its resistome, revealing distinct groups and resistance gene profiles.

This study shows azithromycin synergizes with tetracyclines to overcome minocycline resistance in Acinetobacter baumannii, with synergy observed across strains and media.

This study shows how hospital network structure influences the evolution and spread of drug-resistant bacteria, identifying two distinct evolutionary strategies in Acinetobacter baumannii.

This study analyzes Klebsiella strains from ICU patients to show that within-host genetic changes, not virulence traits, drive progression from gut colonization to bloodstream infections.

The paper reports the first detection of a colistin-resistant ESBL-producing E. kobei strain isolated from a child in Cameroon, highlighting antimicrobial resistance concerns.

This study evaluates graph-based tools for detecting antimicrobial resistance genes in metagenomes, finding that graph alignment improves detection while preserving genomic context.

This study identifies 3,4-dihydroxybenzoic acid (DHBA), a rutin metabolite, as a potent antibiotic potentiator that restores susceptibility in XDR Gram-negative pathogens through a system-wide genetic susceptibility signature.

This paper introduces GL-HAT, a transformer model for predicting antimicrobial resistance and identifying resistance-related genomic contexts, achieving high accuracy and interpretability.

This study shows that MGB-BP-3, a minor groove binder, is more effective against Gram-negative bacteria like E. coli under nutrient-limited conditions than in rich media, with in vivo efficacy in a Galleria mellonella model.

This study uses adaptive laboratory evolution to show that Streptomyces coelicolor develops ciprofloxacin resistance via efflux pumps and increased antibiotic production, with cross-resistance and collateral sensitivity to other drugs.

This study evaluates the accuracy of antimicrobial resistance gene prediction by analyzing bit-score cutoff values in CARD models and identifies sources of over- and underprediction in the RGI tool.

This study analyzes the pangenome structure and ecological adaptation of Klebsiella pneumoniae species complex across different habitats, revealing phylogroup- and niche-specific genomic features.

MetaPointFinder detects antimicrobial resistance-conferring point mutations from metagenomic reads, outperforming existing methods in simulated data.

This paper develops a cross-scale model to study how gut microbiota dynamics influence antimicrobial resistance persistence and transmission across hospital and community settings.

This study examines CRISPR-Cas systems in Klebsiella pneumoniae, finding no significant association between CRISPR-Cas and multidrug resistance, but noting diverse spacers in non-MDR strains targeting ARG-bearing plasmids.

This study shows how mobile genetic elements synergistically drive the stepwise accumulation of antibiotic resistance genes in plasmids, proposing a framework to predict and surveil multidrug resistance.

The paper shows that a shift in pre-existing antibiotic heteroresistance, not de novo mutation, explains a change from susceptible to resistant classification during treatment.

This study characterizes a multidrug-resistant Proteus mirabilis isolate from India, revealing AMR genes, virulence factors, and novel genomic features associated with its pathogenic potential.

This study shows that the gyrBD429N mutation in Neisseria gonorrhoeae confers cross-resistance to gepotidacin and has variable fitness effects across strains.

This study identifies MSMEG_6194 in Mycobacterium smegmatis as a DD-carboxypeptidase, showing that a single amino acid substitution converts its activity to beta-lactamase.

This study identifies mutHV76G in K. pneumoniae causing hypermutation, antibiotic resistance, and plasmid acquisition without fitness cost.

The paper analyzes K. pneumoniae genomes to show convergence between antimicrobial resistance and hypervirulence, identifying dual-risk clones and geographic hotspots.

This study identifies key mutations in PBP2 from a ceftriaxone-resistant Neisseria gonorrhoeae strain that balance resistance and transpeptidase activity.

This paper develops wzi-Seq, a method to detect and quantify Klebsiella strains in gut samples, and finds that mono-colonization is associated with increased infection risk.

This study investigates how carbamylation of lysine and chloride ions affect OXA-143 dynamics and catalytic efficiency using HDX and MD simulations.

This study assesses antimicrobial resistance in little stints and their aquatic ecosystems in Kenya, finding widespread resistance despite no significant differences between high and low impact sites.

This study analyzes KPC-NDM-CRKP isolates to show how mutations, horizontal transfer, and accessory genome plasticity contribute to their epidemiological success.

The paper quantifies the inoculum effect in β-lactamase-expressing bacteria and shows it is governed by a biphasic trade-off between metabolism and growth rate.