Fungal and yeast genetics research

CAYSS automates the analysis of yeast spore segregation data to measure recombination rates and interference from flow cytometry data.

The paper challenges a previous finding by showing that rpl1b deletion mutants are competent for respiration, contradicting the earlier report of a petite phenotype.

This study identifies IRA2 as a key gene for ethanol tolerance in Saccharomyces cerevisiae through QTL mapping and molecular validation.

The paper investigates how priority effects influence gene expression in nectar yeast, finding differential expression in amino acid metabolism and eQTL enrichment for antifungal resistance.

The paper shows that formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient Pichia pastoris, enabling self-induced protein expression without methanol.

This study uses solid-state NMR to analyze the molecular structure of cell walls in Mucorales fungi, revealing the composition and organization of chitin, chitosan, and associated polysaccharides.

This paper introduces an improved tetracycline-inducible expression system for fission yeast that enables control of gene expression in non-dividing cells and supports synchronous meiosis induction.

The paper shows that NSF in fission yeast interacts with Hmt2 and shifts gene expression from fermentation to respiration-like, independent of carbon source.

The paper introduces pSPObooster, a plasmid system that improves sporulation efficiency in S. cerevisiae lab strains by correcting meiotic defects in MKT1 and RME1 genes.

This study identifies three gene clusters in Candida intermedia responsible for lactose and galactose metabolism, revealing a unique regulatory network distinct from other yeasts.

This study identifies beta-1,6-glucan as essential for fungal cell wall structure, integrity, and host-pathogen interactions.

The paper develops improved AMA1-based plasmids for more consistent and stronger gene expression in filamentous fungi, showing increased reporter expression, CRISPRa efficiency, and metabolite production.

This paper develops an efficient gene targeting system in Trichophyton rubrum using a Δku80 strain and shows that Cyp51A contributes to azole drug tolerance by responding to efinaconazole.

The paper biochemically characterizes KpAspE, a Group 5 septin from Knufia petricola, showing it recapitulates functions of canonical septin hetero-octamers in vitro.

This study identifies the Rim/Pal pathway in Malassezia sympodialis as essential for alkaline pH adaptation and demonstrates its role in atopic dermatitis.

This study compares cell wall structure in Aspergillus nidulans and Aspergillus fumigatus using solid-state NMR, revealing similarities and differences in polysaccharide composition and organization.

This study identifies phosphate transporters in Candida albicans and shows how mutations in these genes affect growth and stress resistance under phosphate scarcity.

This paper investigates the role of Eri1 in Candida albicans, showing it inhibits hyphal morphogenesis via a Ras-independent cAMP-PKA pathway and affects virulence.

The paper uses CRISPR-Cas9 to knock out endogenous secreted proteins in Komagataella phaffii, increasing recombinant protein production by 20x.

This study identifies MAR1, an HMG-box protein, as a regulator of n-alkane adsorption and cell morphology in Yarrowia lipolytica through transcriptional control of relevant genes.

This study investigates how mechanical strategies in fungal cell walls support growth and size diversity across species, finding that wall thickness and turgor scale with cell diameter and growth speed.

This study shows that disrupting the PCA pathway in fungi affects vacuolar integrity, drug detoxification, and susceptibility to antifungal drugs, supporting its potential as a therapeutic target.

The paper identifies coactivator proteins that interact with the hyperactive Pdr1 transcription factor in Candida glabrata and shows their necessity for Pdr1-dependent gene transcription and azole resistance.

This study uses high-resolution live cell imaging to characterize sub-cellular structures and dynamic processes in the halotolerant yeast Debaryomyces hansenii, providing a template for studying genetically recalcitrant organisms.

This study examines phenotypic and genotypic variation in Schizophyllum commune strains, finding high diversity in mushroom development and lignocellulose degradation, with conserved genes for these traits across genomes.

The paper shows that TORC1 inhibition increases proteasome assembly chaperone expression in yeast via the Cell Wall Integrity pathway and Sch9 signaling.

This study shows that sirtuins in Aspergillus fumigatus are important for virulence and pathogenesis, with AfSirE deletion reducing virulence and altering protein acetylation and gene expression.

The paper analyzes the pan-transcriptome of yeast isolates to show that accessory genes significantly contribute to gene expression variation across populations.

This study enhances interdomain conjugation efficiency in yeast by leveraging bacterial commensalism and mannoprotein-mediated binding to control and modify yeast populations.

This study identifies the phosphorylation sites on Whi5 and shows how their hypo- and hyper-phosphorylation regulate cell cycle entry and progression in budding yeast.