Advanced Fluorescence Microscopy Techniques

FiPhA is an open-source platform for fiber photometry analysis that simplifies data processing, visualization, and quality control for behavioral neuroscience research.

This study demonstrates that fluorescence lifetime can accurately measure neuromodulator dynamics across animals and over time, overcoming limitations of intensity-based measurements.

This paper introduces a neural network to filter artifacts in fluorescence correlation spectroscopy data, improving analysis of samples with peak artifacts.

This paper introduces a method to map amino acid positions and post-translational modifications in single protein molecules with sub-nanometer resolution using FRET-based single-molecule protein fingerprinting.

FluoroTensor uses deep learning to identify and track colocalized molecules and their stoichiometries in multi-color single molecule imaging.

The paper introduces a novel genetically-encoded sensor for PAC1 receptor activation, demonstrating its utility in vitro and in vivo across species.

The paper develops a Cre-dependent reporter mouse, FL-AK, for quantitative imaging of PKA activity dynamics in vivo and ex vivo.

The paper introduces an mRNA-based method to express chromobodies for tracking endogenous proteins in living cells, showing it reduces background fluorescence compared to plasmid transfection.

This study uses TCSPC imaging to show that CDN1163, a SERCA2a activator, synergizes with ATP to shift SERCA2a toward a competent conformation, enhancing its function under physiological conditions.

This paper develops a deformable mirror-based remote focusing system to enable high-accuracy whole-cell super-resolution imaging with uniform resolution across thick samples.

This paper introduces a fast and robust 3D MINFLUX excitation module using a variable phase plate, achieving record speed and performance.

The paper develops a combined microscope platform that integrates quantitative phase contrast and super-resolution fluorescence imaging for high-sensitivity visualization of sub-cellular structures and molecules in their cellular context.

The paper improves a split reporter for detecting protein-protein interactions using orthology-based protein engineering, resulting in a brighter, more reliable reporter with higher dynamic range.

This paper introduces a data-acquisition module for fluorescence LSM that enhances single-photon imaging capabilities, enabling super-resolution and multi-species imaging.

This paper develops a closed-loop optoelectronic system to precisely control molecular activities in live cells, demonstrating spatiotemporal manipulation of reactive oxygen species and tubulin polymerization.

This paper provides an end-to-end workflow for non-destructive 3D pathology using light-sheet microscopy, focusing on fluorescent H&E analogs for preclinical and clinical studies.

This paper introduces tMAVEN, a software platform for analyzing single-molecule data using multiple kinetic models to improve accuracy by addressing mismatches between models and molecular mechanisms.

This paper introduces SpinFlux, a modulation-enhanced spinning disk confocal microscopy method that improves localization precision over SMLM with minimal hardware modifications.

PyF2F measures fluorophore-to-fluorophore distances from PICT data, validated on a published dataset.

The paper develops synthetic binders specific to PSD-95 for imaging endogenous proteins with minimal impact on function and compatibility with super-resolution techniques.

The paper introduces a method to map individual sensory nerve axons from digits to spinal cord at sub-micron resolution.

This study demonstrates the feasibility of 4- and 5-color fluorescence imaging in Dictyostelium discoideum using new fluorescent proteins and labels.

nanoFeatures is a cross-platform tool for quantitative analysis of super-resolution microscopy images of nanoparticles, extracting features like size, shape, and molecular abundance.

This paper develops an ultrahigh-throughput SMLM methodology using a color glass filter to achieve sub-10 nm localization and sub-5 nm spectral precision for multicolor imaging.

The paper develops a four-color single-molecule imaging system using fluorescent HiBiT peptides to track GPCR dynamics and interactions in living cells.

The paper describes the design of a super-resolution fluorescence cryomicroscope for single molecule localization microscopy of cryosamples.

This paper optimizes light-sheet fluorescence microscopy illumination beams using deep learning to improve cell detection performance.

This paper demonstrates how deep UV wavelengths can recover STED bleaching and enable reactivation of fluorophores for correlative STED and SMLM imaging.

This paper introduces a high-speed 3DSIM system using DMD and electro-optic modulators, achieving improved resolution and imaging speed for various biological specimens.

This study evaluates how the tilt of a coverslip affects STED and two-photon microscopy image quality and proposes a method to correct the tilt.