Advanced Fluorescence Microscopy Techniques

The paper uses three-photon microscopy to correlate structural and functional features in visual cortical areas of awake mice.

The paper introduces a method to label proteins in Drosophila embryos using FRET reporters, genomic integration, and GAL4-responsive expression to study protein interactions in situ.

This study shows that controlling emulsification in organic solvent-based tissue clearing improves transparency and preserves fluorescence without complete dehydration.

The paper introduces a new genetically encoded voltage indicator, SomArchon, enabling high-speed, sensitive neural activity imaging in awake mice across multiple brain regions.

This paper introduces ED-LTFM to overcome tissue scattering in wide-field deep imaging, demonstrating improved imaging depth in mouse brain in vivo.

The paper introduces mesoSPIM, an open-source light-sheet microscope initiative for imaging large cleared tissue samples with near-isotropic resolution.

The paper describes analytical methods for quantitatively analyzing the 3D distribution and trans-synaptic alignment of proteins using single-molecule localization microscopy data.

The paper introduces a dual-plane mesoscopic imaging system that improves imaging throughput by combining temporal-division multiplexing and remote focusing.

The paper develops a biophysical model of GCaMP6s calcium binding kinetics to accurately infer action potentials from fluorescence data in vivo.

Structure-guided mutagenesis of GCaMP6 plus neuron-based screening yields a jGCaMP7 family, each variant tuned to a different regime: single spikes, neurites and neuropil, or population imaging.

The paper explains an unexplained discrepancy in photon count ratios used to estimate axial position in single-molecule localization microscopy and shows susceptibility to optical aberrations.

The paper introduces a Monte Carlo method to quantify uncertainty in motion type estimation for single-particle tracking, addressing error propagation in bioimage analysis.

The paper optimizes the DsRed fluorescent protein for expression in Mycobacterium tuberculosis and confirms its functionality with full-length constructs.

The paper introduces a new imaging technique to enhance fluorescent signal in cleared mouse bodies, enabling whole-body visualization of neuronal connectivity and trauma effects.

The paper demonstrates that three-photon microscopy at 1300-nm enables whole-brain imaging of living Drosophila with reduced aberration and scattering compared to one- and two-photon methods.

This paper shows that two-photon imaging causes brain heating and increased calcium activity in astrocytes, and that continuous-wave illumination is as damaging as pulsed light.

This paper introduces a holographic imaging technique through a 50 μm-core multimode fibre to achieve high-resolution deep-brain imaging with minimal tissue damage in living mice.

This study demonstrates that Bessel beams improve the sensitivity and accuracy of functional imaging in light-sheet microscopy by reducing artifacts and enhancing detection of calcium transients and activity correlations.

Suite2p processes two-photon calcium movies faster than real time on desktop workstations, roughly doubling curated cell yield over CNMF and recovering 13,451 neurons in one session.

This paper develops a method for deep-brain neurotransmitter imaging using two-photon excitation of near-infrared nanosensors, showing reduced scattering and improved image quality.

This paper introduces a new light-sheet microscopy technique for high-resolution volumetric imaging of whole mouse brains.

This paper introduces a blind sparse deconvolution algorithm for inferring spike trains from fluorescence recordings, showing improved robustness, speed, and accuracy over existing methods.

The paper introduces SWIFT, a computational imaging method for capturing coordinated neuronal activity across large tissue volumes in mammals at high speed and resolution.

This paper introduces a novel microendoscope using ultrathin optical fibers for deep brain imaging with micron-scale resolution and improved speed in vivo.

The paper evaluates and compares two-photon calcium imaging modalities to optimize imaging speed and signal-to-noise ratio in scattering brain tissue.

This paper develops Bayesian image processing methods for super-resolution microscopy that improve resolution while maintaining computational efficiency.