Plant biochemistry and biosynthesis

Oxidation of Four Monoterpenoid Indole Alkaloid Classes by Three Cytochrome P450 Monooxygenases from Tabernaemontana litoralis

This study identifies and characterizes three new CYPs in T. litoralis that catalyze specific oxidations of four MIA skeletons, expanding understanding of MIA biosynthesis.

Modeling the effect of daytime duration on the biosynthesis of terpenoid precursors

This study models how circadian rhythms affect terpenoid precursor biosynthesis in plants, showing that combined regulation maintains stable concentrations despite daylight variations.

Biosynthetic Origin of the Methoxy Group in Quinine and Related Cinchona Alkaloids

The paper identifies the biosynthetic origin of the methoxy group in quinine and related Cinchona alkaloids, showing it is introduced onto tryptamine and demonstrating the pathway in planta.

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus

The paper constructs a cell-type-aware gene regulatory network for MIA biosynthesis in C. roseus using single-cell multi-omics data, identifying a novel idioblast-specific TF that regulates late-stage biosynthetic genes.

Universal function of WRI1 involved in integrated regulation of multiple latex metabolism pathways in Natural Rubber

This study identifies WRI1 orthologues in rubber-producing plants that enhance natural rubber quality and production through regulation of lipid metabolism and biosynthesis pathways.

Genome-based discovery of pachysiphine synthases in Tabernaemontana elegans

This paper identifies and characterizes pachysiphine synthase genes in Tabernaemontana elegans using genome sequencing and computational analysis, enabling engineered yeast production of MIAs.

Crystal Structure of Caryolan-1-ol Synthase, a Sesquiterpene Synthase Catalyzing an Initial Anti-Markovnikov Cyclization Reaction

The paper solves the crystal structure of caryolan-1-ol synthase with and without a substrate analog, revealing structural changes that suggest a mechanism for anti-Markovnikov cyclization.

Streamlined screening platforms lead to the discovery of pachysiphine synthase from Tabernanthe iboga

The paper identifies two CYP450 enzymes in Tabernanthe iboga responsible for oxidative modifications of tabersonine scaffolds using a streamlined screening strategy in Nicotiana benthamiana.

CYP76BK1 orthologs catalyze furan and lactone ring formation in clerodane diterpenoids across the mint family

This paper identifies and functionally characterizes CYP76BK1 orthologs across Lamiaceae subfamilies, showing their role in furanoclerodane biosynthesis and evolutionary conservation.

A chromosome level reference genome of Diviners sage (Salvia divinorum) provides insight into salvinorin A biosynthesis

The paper presents a chromosome-level genome of Salvia divinorum, identifying gene clusters involved in salvinorin A biosynthesis.

Parallel evolution of methyltransferases leads to vobasine biosynthesis in Tabernaemontana elegans and Catharanthus roseus

This study identifies and characterizes a new gammaTMT-like enzyme from Tabernaemontana elegans, showing parallel evolution of methyltransferases leading to perivine N-methylation in two plant species.

Exogenous stimulation of Tanacetum vulgare roots with pipecolic acid leads to tissue-specific responses in terpenoid composition

This study examines how pipecolic acid treatment affects terpenoid composition in different tissues of tansy plants, revealing tissue-specific responses and metabolic differentiation.

Comparative metabolomics of wild and cultivated yew: insights into metabolic diversity and environmental adaptability

This study compares metabolomes of wild and cultivated T. cuspidata, finding wild species have higher metabolic diversity and environmental adaptability due to genetic resources.

Three cytochrome P450 from Nicotiana attenuata play key roles in triterpene biosynthesis

Three CYP716 enzymes in Nicotiana attenuata catalyze distinct steps in triterpene biosynthesis, influencing growth and development through ABA and stress responses.

Integrative multi-omics data elucidating the biosynthesis and regulatory mechanisms of furanocoumarins in Angelica dahurica

This study identifies biosynthesis pathways and regulatory mechanisms of furanocoumarins in Angelica dahurica using multi-omics data and experimental validation.

Molecular basis of one-step methyl anthranilate biosynthesis in grapes, sweet orange, and maize

The paper identifies one-step anthranilate methyltransferases in grapes that synthesize methyl anthranilate, revealing distinct active site residues compared to maize and citrus enzymes.

De Novo Transcriptome Analysis Reveals Novel Insights into Secondary Metabolite Biosynthesis in Tylophora indica (Burm. f) Merrill.

This study performs transcriptome analysis of Tylophora indica to identify genes involved in secondary metabolite biosynthesis and genetic diversity.

Steroidal glycoalkaloid biosynthesis and fungal tolerance are regulated by ELONGATED HYPOCOTYL 5, SlHY5, in tomato

This study shows that the light-regulated transcription factor SlHY5 controls steroidal glycoalkaloid biosynthesis and fungal tolerance in tomato.

A green alternative to fragrant agarwood sesquiterpenoid production

This paper develops a sustainable method to produce agarwood-like sesquiterpenoids using synthetic biology in algae, avoiding the need for harvesting endangered trees.

Ketocarotenoid production in tomato triggers metabolic reprogramming and cellular adaptation: The quest for homeostasis?

The paper investigates how ketocarotenoid production in tomato triggers metabolic reprogramming and cellular adaptation to maintain homeostasis.

De novo Sequencing and Whole Transcriptome Analysis for Biosynthesis Pathway of Diosgenin in Pedalium murex L.: A Medicinal herb

This study performs transcriptome analysis of Pedalium murex to identify genes involved in diosgenin biosynthesis and provides transcriptome data for functional genomics research.

Natural gene variation in Cannabis sativa unveils a key region of cannabinoid synthase enzymes

The paper identifies three mutations in cannabinoid oxidocyclases that affect enzyme activity and product specificity, revealing a key region for catalytic function.

Assessment of tobacco and N. benthamiana as biofactories of irregular monoterpenes for sustainable crop protection.

The paper evaluates tobacco and N. benthamiana as biofactories for producing irregular monoterpenes like chrysanthemol and lavandulol, demonstrating their potential for sustainable pest control through volatile emissions.

Haplotype-phased genome revealed the butylphthalide biosynthesis and hybrid origin of Ligusticum chuanxiong

This study presents the haplotype-resolved genome of Ligusticum chuanxiong, identifies enzymes involved in butylphthalide biosynthesis, and reveals its hybrid origin.

The characterisation of phytoene synthase-1 and 2, and 1-D-deoxy-xylulose 5-phosphate synthase genes from red chilli pepper (Capsicum annuum)

The paper identifies allelic variation in PSY and DXS genes associated with carotenoid content and fruit color intensity in red chilli peppers.

Genomic description of critical upstream cannabinoid biosynthesis genes

The paper annotates and explores variation in cannabinoid biosynthesis genes across multiple Cannabis sativa genomes, identifying copy number variation and exon usage differences.

Functional characterization of five triterpene synthases through De-novo assembly and transcriptome analysis of Euphorbia grantii and Euphorbia tirucalli

The paper functionally characterizes five triterpene synthases from Euphorbia species and develops a yeast strain for large-scale triterpene production.

Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)

This study elucidates the biosynthesis of unique benzylisoquinoline alkaloids in sacred lotus and enables their microbial synthesis.

Translational fusion of terpene synthases enhances metabolic flux by increasing protein stability

This study shows that fusing terpene synthases increases metabolic flux by boosting enzyme stability and expression, leading to higher nerolidol production.

Production of 130 diterpenoids by combinatorial biosynthesis in yeast

The paper reports the production of 130 diterpenoids in yeast through combinatorial biosynthesis using ten diterpene synthases and four cytochrome P450 enzymes, with 80 novel compounds identified.