Discover, rate, and verify biology preprints.

EC-isHCR: a rapid method for in situ hybridization chain reaction in diverse animal samples

This paper demonstrates that EC-isHCR retains the features of conventional isHCR and introduces an automated probe design tool to enhance its applicability across diverse samples.

Liquid crystalline mesophase spacing as a quantitative predictor of release kinetics for co-loaded hydrophilic and hydrophobic payloads

This paper shows that mesophase spacing in liquid crystalline nanocarriers predicts hydrophilic payload release kinetics, linking material composition to functional performance.

Benchmarking and behavioral characterization of LLM agents for protein design

This paper benchmarks LLM agents in protein design tasks, finding they outperform hardcoded pipelines but underperform expert practice due to limited evaluation depth.

Matrix viscoelasticity regulates dermal fibroblast activation in a three-dimensional fibrillar microenvironment

This study shows that fibroblast activation is influenced by matrix viscoelasticity in 3D fibrillar microenvironments, with slow stress relaxation and stiffness promoting activation and ECM remodeling.

Magnetic Levitation of Intact Tumor Biopsies Reveals Multivariate Biophysical Signatures of Breast Cancer Aggressiveness

This paper applies magnetic levitation to breast cancer biopsies to generate biophysical signatures linked to tumor aggressiveness and clinical subtypes.

A Multiscale Computational Analysis of Myometrial Excitation during Late Pregnancy

This study develops a multiscale computational model to simulate myometrial excitation during late pregnancy, showing that ion channel regulation determines preterm uterine excitability.

Improving oral dissolution kinetics of weakly basic vodobatinib via slurry conversion to an amorphous drug-polymer salt

This paper improves the oral dissolution of vodobatinib by converting it into an amorphous drug-polymer salt using slurry conversion, achieving a 35-fold increase in dissolution rate.

FRAGMENT MORPHOMETRY ANALYSIS AND SAME-COLOR-CHANNEL SEPARATION ENABLE OBJECTIVE QUANTIFICATION ACROSS BBB MODELS

The paper introduces two image-analysis pipelines to objectively quantify BBB integrity in vitro and in vivo, showing reduced ZO-1 fragment area and fragmentation ratio in glycocalyx knockdown models and altered markers in aged and Alzheimer's mice.

Fluorescence Properties of Collagen Types I-V: A Comprehensive Study of Spectral and Lifetime Characteristics

This study characterizes the fluorescence properties of collagen Types I-V under different conditions, identifying distinct spectral and lifetime signatures for tissue characterization.

RNA post hoc loading into empty lipid nanoparticles occurs on millisecond timescales during turbulent mixing

The paper investigates RNA post hoc loading into empty lipid nanoparticles using turbulent mixing and finds it occurs within 12 ms under optimal conditions.

System Identification and Control for Optogenetics in Mammalian Nucleocytoplasmic Transport

This paper benchmarks predictive modeling and control techniques with optogenetics to steer nucleocytoplasmic transport in mammalian cells, showing robust performance against cell-to-cell variation.

Reconstruction of critical-sized mandibular defects in a sheep model using a PLLA-PGA-CC scaffold

This study evaluates PLLA-PGA-CC scaffolds for critical-sized mandibular defect repair in sheep, finding enhanced bone regeneration compared to controls but limited osteoconductivity.

Expression-linked promoter selection (ELiPS) engineers short, strong ubiquitous promoters for gene therapy applications

ELiPS generates and screens short, strong promoters for gene therapy, outperforming existing promoters in vitro and enabling therapeutic expression in vivo.

Active Learning-Guided Peptide Design for Modulating Condensate Properties upon Recruitment

The paper uses active learning to design peptides that modulate the physical properties of a biomolecular condensate by interacting with MUT-16.

Conversion of a Viral Glycan Shield into a Binding Anchor via Causal-Driven Antibody Optimization

This paper develops AENCS to engineer antibodies targeting glycosylated epitopes, demonstrating ACC01's 24-fold improved neutralization against SARS-CoV-2 variants.

Cerebrovascular Imaging-to-Graph Reconstruction for Individualized Digital Twin Brains

This paper introduces CVIG, a framework to reconstruct cerebrovascular graphs from in vivo images, enabling high-fidelity brain biophysical simulations for digital twin applications.

Comparing metabolic engineering scenarios using simulated design-build-test-learn-cycles

This study uses in-silico DBTL cycles to evaluate how process parameters affect strain optimization outcomes in metabolic engineering.

High-Yield Recovery of Reactive Nitrogen as Cyanophycin by Engineering Acinetobacter baylyi ADP1 under Wastewater-Relevant Conditions

This study engineers Acinetobacter baylyi to produce cyanophycin from wastewater nutrients, achieving high yields without arginine supplementation and expanding temperature tolerance.

BIRD-Seq: B2 Protein Integrated End-to-End Pipeline for dsRNA Detection and Nanopore Sequencing for Virus Monitoring

This paper introduces BIRD-Seq, a dsRNA detection and sequencing pipeline using engineered B2 protein and nanopore sequencing, achieving sub-ng mL-1 sensitivity and enabling virus identification without RNA extraction.

An in vivo platform to jointly monitor cellular and metabolic responses to chemotherapy.

The paper introduces a spatial pharmacology platform to study how chemotherapy affects immune and metabolic states in tumors, identifying key metabolic axes and resistant macrophage populations.