A. C. Kruse

Antibodies Expand the Scope of Angiotensin Receptor Pharmacology

This study develops nanobody antagonists against the AT1R receptor, showing they can selectively bind and modulate the receptor with tunable ligand selectivity.

A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation

This study maps B cell co-receptor signaling dynamics using proximity labeling and mass spectrometry, identifying new mediators of B cell activation and metabolic reprogramming.

Allosteric activation of cell wall synthesis during bacterial growth

This study identifies a dynamic structural mechanism in a bacterial cell wall synthase that couples polymerization and crosslinking during cell elongation.

Heterogeneity of Tethered Agonist Signaling in Adhesion G Protein-Coupled Receptors

The paper profiles tethered agonist (TA) signaling in all 33 human aGPCRs using a platform across assays, revealing heterogeneous responses and aligning with AlphaFold2 predictions.

Engineering and characterization of a long half-life relaxin receptor RXFP1 agonist

This study engineers a relaxin-2 variant with a longer serum half-life by fusing it with an antibody Fc fragment, maintaining RXFP1 agonist activity in vitro and in vivo.

The relaxin receptor RXFP1 signals through a mechanism of autoinhibition

This study determines the cryo-EM structure of active-state RXFP1 bound to relaxin-2 and Gs protein, revealing an autoinhibition mechanism.

Metal cofactor stabilization by a partner protein is a widespread strategy employed for amidase activation

The paper shows that ActH stabilizes metals in the LytH active site to activate amidase activity, revealing a general strategy for amidase activation.

Crystal structures of the σ2 receptor template large-library docking for selective chemotypes active in vivo

This study uses crystal structures of the σ2 receptor to dock and screen a large library, identifying new chemotypes with high affinity and selectivity for the receptor.

Rapid generation of potent antibodies by autonomous hypermutation in yeast

This paper introduces AHEAD, a yeast-based system for rapid antibody generation through autonomous hypermutation, achieving high-affinity antibodies against SARS-CoV-2 and other targets in just 2 weeks.

Oral administration of a single anti-CfaE nanobody provides broadly cross-protective immunity against major pathogenic Enterotoxigenic Escherichia coli strains

This study shows that oral administration of a single nanobody provides broad protection against multiple ETEC strains by targeting conserved epitopes.

Virtual screening identifies novel high-affinity σ1 receptor ligands

The paper uses virtual screening to identify novel high-affinity σ1 receptor ligands, finding 77% of tested compounds bind with high affinity.

Structural basis for sigma-1 receptor ligand recognition

This study presents crystal structures and MD simulations of the sigma-1 receptor bound to ligands, revealing agonist-induced structural changes and a multistep binding mechanism.

Structure and mutagenic analysis of the lipid II flippase MurJ from Escherichia coli

The paper reports the crystal structure of MurJ from E. coli, performs mutagenesis to map functional sites, and identifies co-evolved interactions supporting a rocker-switch model for lipid II transport.

Platform for rapid nanobody discovery in vitro

This paper presents an in vitro platform for rapid nanobody discovery using yeast surface display, demonstrating its application to various antigens including lipid flippase and G protein-coupled receptors.