J. A. Hirota

Irreversible PDMS bonding using flame activation of adhesives for fabrication of microfluidic and organ-on-chip devices

This paper introduces a low-cost, flame-based method for irreversible PDMS bonding, enabling robust microfluidic and organ-on-chip fabrication with high bonding integrity.

AngioPlate - Biofabrication of perfusable complex tissues in multi-well plates with 4D subtractive manufacturing

The paper introduces a 4D subtractive manufacturing technique to create perfusable complex tissues in multi-well plates, enabling the fabrication of vascular networks and organ-specific structures.

Development and validation of a mouse model of contemporary cannabis smoke exposure

This paper develops and validates a mouse model of cannabis smoke exposure, showing changes in immune cell populations and mediators in both male and female mice.

Potentiation of Long-Acting β2-Agonist and Glucocorticoid Responses in Human Airway Epithelial Cells by Modulation of Intracellular cAMP

This study shows that inhibiting PDE4 or ABCC4 enhances the anti-inflammatory effects of LABA/GCS in human airway epithelial cells exposed to viral stimuli.

Surface modification of PDMS-based microfluidic devices with collagen using polydopamine as a spacer to enhance primary human bronchial epithelial cell adhesion

This paper develops methods to covalently bind collagen to PDMS microfluidic devices using polydopamine, enhancing cell adhesion for organ-on-a-chip applications.

PHAIR, A biosensor for pH measurement in air-liquid interface of human airway epithelial cells

The paper introduces PHAIR, a pH sensor for measuring pH in human airway epithelial cells, showing it does not affect cell viability or cytokine responses.

Development and validation of an open-source, disposable, 3D-printed in vitro environmental exposure system for Transwell culture inserts

The paper develops and validates an open-source 3D-printed in vitro system to study cannabis smoke exposure on human airway epithelial cells, showing changes in morphology and cytokine expression without significant cytotoxicity.

Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells

This study tests cAMP modulation via ABCC4 and PDE-4 inhibition as an add-on therapy to VX-770 to increase CFTR activity in human airway epithelial cells.

Experimental and natural evidence of SARS-CoV-2 infection-induced activation of type I interferon responses

This study shows SARS-CoV-2 infection induces a mild type I IFN response in vitro and in moderate COVID-19 cases, and cannot inhibit exogenous IFN induction.

Isolation, sequence, infectivity and replication kinetics of SARS-CoV-2

The paper isolates SARS-CoV-2 from two patients, sequences the virus, and tests its infectivity in human immune cells, finding that PBMCs are not permissive to infection.

Gene expression and in situ protein profiling of candidate SARS-CoV-2 receptors in human airway epithelial cells and lung tissue

The paper examines gene expression and protein localization of potential SARS-CoV-2 receptors in human airway epithelial cells and lung tissue, finding low ACE2 expression and confirming other candidate receptors.

Expression of the endocannabinoid system in the human airway epithelial cells: Impact of sex and chronic respiratory disease status

The paper analyzes gene expression of the endocannabinoid system in human airway epithelial cells, showing differential expression by disease status and minimal sex impact.

ABCF1 regulates dsDNA-induced immune responses in human airway epithelial cells

This study identifies ABCF1 as a cytosolic nucleic acid sensor in human airway epithelial cells that modulates antiviral responses and interacts with TLR signaling.