J. Hirst

Structural basis of a regulatory switch in mammalian complex I

This study defines the structural basis of a regulatory switch in mammalian complex I by combining cryoEM structures with biochemical analysis, showing how conformational changes mediate deactivation during ischaemia.

Structural insights into complex I deficiency and assembly from the disease-related ndufs4-/- mouse

This study investigates the structural and functional consequences of NDUFS4 deficiency in a mouse model, revealing impaired complex I assembly and highlighting the role of NDUFAF2 in module recruitment.

The antipsychotic medications aripiprazole, brexpiprazole and cariprazine are off-target respiratory chain complex I inhibitors

This study shows that antipsychotics aripiprazole, brexpiprazole, and cariprazine inhibit mitochondrial respiratory complex I, causing toxicity and movement disorders.

Cryo-EM structures of mitochondrial respiratory complex I from Drosophila melanogaster

The paper determines the Cryo-EM structure of Drosophila melanogaster mitochondrial respiratory complex I, revealing structural homology to mammalian complex I and identifying a novel subunit homologue.

Structural Basis of Mammalian Respiratory Complex I Inhibition by Medicinal Biguanides

This study identifies how biguanides inhibit respiratory complex I by binding in the quinone channel and causing a chaotropic effect.

Accurate delineation of individual tree crowns in tropical forests from aerial RGB imagery using Mask R-CNN

This paper introduces Detectree2, a deep learning model that accurately segments individual tree crowns in tropical forests from aerial RGB imagery, enabling growth and mortality analysis of large trees.

Superinfection exclusion creates spatially distinct influenza virus populations

This study shows that superinfection exclusion (SIE) creates spatially distinct influenza virus populations, limiting genome interactions between virus subpopulations as they spread within host tissues.

Stable and ancient endocytic structures navigate the complex pellicle of apicomplexan parasites

This study identifies stable endocytic structures in Toxoplasma, showing their conservation across Apicomplexa and their role in plasma membrane homeostasis rather than nutrition.

Cryo-EM structures reveal ubiquinone-10 binding to mitochondrial complex I and conformational transitions associated with Q-site occupancy

The paper presents cryo-EM structures of mitochondrial complex I with and without ubiquinone-10, revealing conformational changes associated with Q-site occupancy.

Complexome profile of Toxoplasma gondii mitochondria identifies a divergent cytochrome bc1 complex

This study identifies new subunits of Toxoplasma gondii's mitochondrial complex III using complexome profiling, revealing their essential role in parasite survival and drug target potential.

Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 Complex

The paper shows that AP-5/SPG11/SPG15 complex recruitment to late endosomes/lysosomes requires PI3P and Rag GTPases, linking it to mTORC1 pathway and autophagic lysosome reformation.

AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A

The paper identifies AP-4's role in ATG9A trafficking via RUSC2-mediated vesicle transport, linking AP-4 deficiency to autophagy dysregulation in multiple cell types.

Structure of the deactive state of mammalian respiratory complex I

This study determines the structure of the deactive state of Bos taurus complex I using cryo-EM, revealing structural changes associated with the transition from active to deactive states.