J. R. Engen

Impact of the clinically approved BTK inhibitors on the conformation of full-length BTK and analysis of the development of BTK resistance mutations in chronic lymphocytic leukemia.

This study examines how different BTK inhibitors affect BTK conformation and explains resistance mutation patterns in CLL patients.

Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states

This study presents the first structural analysis of full-length BTK, revealing conformational heterogeneity in the PHTH-PRR region and providing insights into BTK activation mechanisms.

Role of Complementarity-Determining Regions 1 and 3 in Pathologic Amyloid Formation by Human Immunoglobulin κ1 Light Chains

This study investigates how mutations in CDR1 and CDR3 regions of human immunoglobulin κ1 light chains influence amyloid formation through structural and dynamic analyses.

Increase the flow rate and improve hydrogen deuterium exchange mass spectrometry

This paper improves hydrogen deuterium exchange mass spectrometry by increasing flow rates under quench conditions, enhancing separation efficiency and deuterium recovery.

Resolving chaperone-assisted protein folding on the ribosome at the peptide level

This study uses HDX-MS to map the cotranslational folding pathway of E. coli dihydrofolate reductase, revealing structured intermediates and chaperone interactions on the ribosome.

Von Willebrand factor A1 domain affinity for GPIbα and stability are differentially regulated by its O-glycosylated N-linker and C-linker

This study investigates how O-glycosylated N- and C-linkers regulate the affinity and stability of the A1 domain of von Willebrand factor for GPIbα, revealing differential effects on binding and structural dynamics.

Translocation of polyubiquitinated protein substrates by the hexameric Cdc48 ATPase

This study elucidates how the Cdc48 ATPase complex unfolds and translocates polyubiquitinated protein substrates through cooperative binding with Ufd1/Npl4.

Allosteric control of Ubp6 and the proteasome via a bidirectional switch

This study identifies an allosteric switch that controls Ubp6 and the proteasome, showing their active states are mutually exclusive.

Protection of the prodomain 1-helix correlates with latency in the transforming growth factor- family

The paper investigates the structural basis of latency in TGF-beta family members by comparing conformational states and hydrogen-deuterium exchange patterns across multiple family members.

Crystal Structure of the Werner's Syndrome Helicase

This study determines the crystal structure of the WRN helicase core and identifies structural features that may aid in designing inhibitors against WRN helicase.

Mitochondrial ClpX activates an essential metabolic enzyme through partial unfolding

The paper investigates how mitochondrial ClpX partially unfolds ALAS to activate it by gating cofactor access.

Fusion surface structure, function, and dynamics of gamete fusogen HAP2

This study characterizes the structure, function, and dynamics of the gamete fusogen HAP2, revealing dynamic regions and structural features critical for fusion.

Tolloid cleavage activates latent GDF8 by priming the pro-complex for dissociation

This study shows Tolloid cleavage activates latent GDF8 by destabilizing prodomain-growth factor interfaces and priming the growth factor for release.