N. L. Fawzi

Expanding the molecular grammar of polar residues and arginine in FUS prion-like domain phase separation and aggregation

The paper expands the molecular grammar of polar residues and arginine in FUS prion-like domain phase separation and aggregation by showing additional residues contribute to these processes.

Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates

This study shows that TDP-43 aggregation in stress granules requires up-concentration and oxidative stress, leading to intra-condensate demixing and pathological aggregates.

Small molecule modulation of a redox-sensitive stress granule protein dissolves stress granules with beneficial outcomes for familial amyotrophic lateral sclerosis models

The paper identifies lipoamide as a small molecule that dissolves stress granules and improves outcomes in ALS models by modulating redox-sensitive proteins.

Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates in Xenopus oocytes

Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation

This study investigates the structural and interaction details of Efg1's prion-like domains during phase separation in Candida albicans, revealing helical structures and residue-specific interactions critical for the transcriptional switch between white and opaque cell states.

Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains

The paper identifies short RNAs that prevent and reverse pathological fibrillization of RNA-binding proteins like FUS and TDP-43, showing therapeutic potential for ALS/FTD.

Aliphatic residues contribute significantly to the phase separation of TDP-43 C-terminal domain

This study identifies aliphatic residues as critical contributors to TDP-43 C-terminal domain phase separation, expanding the molecular understanding of protein phase separation.

Molecular insights into the effect of hexanediol on FUS phase separation

This study investigates how alkanediols, particularly 1,6-hexanediol, disrupt FUS phase separation using multiple techniques and identifies factors like geometry and hydrophobicity that influence their effectiveness.

A predictive coarse-grained model for position-specific effects of post-translational modifications on disordered protein phase separation

This paper develops a coarse-grained model to predict how position-specific post-translational modifications affect disordered protein phase separation.

The oncogenic transcription factor FUS-CHOP can undergo nuclear liquid-liquid phase separation

This study shows the FUS-CHOP fusion protein undergoes nuclear liquid-liquid phase separation, forming condensates at super enhancer sites, suggesting a novel oncogenic mechanism in myxoid liposarcoma.

An enhancer sequence in the intrinsically disordered region of the essential cell division protein FtsZ promotes conformation-guided substrate processing by ClpXP in Escherichia coli

The paper shows that an intrinsically disordered region of FtsZ enhances ClpXP-mediated degradation through conformational interactions.

Phase separation of the LINE-1 ORF1 protein is mediated by the N-terminus and coiled-coil domain

This paper shows that the N-terminus and coiled-coil domain of ORF1 protein mediate phase separation, which may protect L1 transcripts from degradation.

Molecular details of protein condensates probed by microsecond-long atomistic simulations

The paper uses all-atom simulations to study protein condensates, revealing structural and dynamic details of phase separation in two IDPs and showing agreement with experiments.

SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation stimulated by RNA and partitions into phases of human ribonucleoproteins

This study shows SARS-CoV-2 nucleocapsid protein undergoes RNA-stimulated liquid-liquid phase separation, forming condensates with host RNA-binding proteins, which may aid viral genome packaging and host protein co-option.

Membrane bending by protein phase separation

This paper investigates how liquid-like protein assemblies on membranes drive membrane bending by creating compressive stress, leading to the formation of membrane tubules.

Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning & reduces neurodegeneration

The paper shows tyrosine phosphorylation of hnRNPA2 reduces granule component partitioning and neurodegeneration in a C. elegans model.

TDP-43 α-helical structure tunes liquid-liquid phase separation and function

This study shows that mutations in TDP-43's helical region modulate its phase separation and function, with implications for ALS and synthetic biology.