S. K. Kim

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

This study uses CRISPR/Cas9 RNP to edit regulatory DNA elements in human islet cells, revealing their role in diabetes risk and β cell function.

Structure and dynamics of the essential endogenous mycobacterial polyketide synthasePks13

This paper determines the structure and dynamics of the mycobacterial polyketide synthase Pks13 using cryoEM and complementary techniques, revealing intermediate states and substrate binding.

Bidirectional multiciliated cell extrusion is controlled by Notch driven basal extrusion and Piezo 1 driven apical extrusion.

The paper identifies two distinct mechanisms for multiciliated cell extrusion in Xenopus embryos: Notch-driven basal extrusion and Piezo1-driven apical extrusion, with age-dependent regulation.

30-Minute Highly Multiplexed VaxArray Immunoassay for Pneumococcal Vaccine Antigen Characterization

The paper introduces a 30-minute multiplexed immunoassay for identifying and quantifying pneumococcal vaccine antigens with high specificity and precision.

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

This paper introduces magneto-gas vesicles (MGVs) for non-invasive in vivo imaging of tissue mechanics, showing improved sensitivity and resolution compared to existing methods.

Islet transplantation tolerance in animals with defined histocompatibility and diabetes

This paper develops a mouse model for studying islet transplantation tolerance by creating a congenic line that allows controlled diabetes induction and assessment of islet engraftment.

Tubulin Acetylation Promotes Penetrative Capacity of Cells Undergoing Radial Intercalation

This study shows that tubulin acetylation enhances the penetrative capacity of cells during radial intercalation in Xenopus embryos by influencing their ability to insert into epithelial barriers.

Heterogenous impairment of α-cell function in type 2 diabetes is linked to cell maturation state

This study identifies heterogeneous α-cell dysfunction in type 2 diabetes linked to impaired maturation and electrophysiological changes.

Distinct autoinhibitory mechanisms regulate vinculin binding by alpha-T-catenin and alpha-E-catenin

This study identifies distinct autoinhibitory mechanisms in T-catenin and E-catenin regulating vinculin binding, showing that T-catenin's N-terminus maintains M-region autoinhibition.

Discovery of ciliary G protein-coupled receptors regulating pancreatic islet insulin and glucagon secretion

The paper identifies ciliary GPCRs in pancreatic islet cells that regulate insulin and glucagon secretion, with FFAR4 and PTGER4 as key targets for diabetes therapy.

In vivo studies of glucagon secretion by human islets transplanted in mice

This study uses CRISPR/Cas9 to create mice lacking human glucagon to study human islet glucagon secretion in vivo after transplantation.