S. H. Kim

Hidden route of protein damage through confined oxygen gas

The paper identifies a hidden oxidative damage pathway in proteins through O2 confinement and photoexcitation, bypassing antioxidant defenses.

Asymmetric Allostery in Estrogen Receptor-α Homodimers Drives Responses to the Ensemble of Estrogens in the Hormonal Milieu

This paper shows that estrogen receptor-α homodimers can function as conformational heterodimers, integrating different ligand signals to produce distinct agonist or antagonist activity profiles.

Brain-wide neuronal circuit connectome of human glioblastoma

This study shows human glioblastoma cells integrate into brain-wide neuronal circuits, exhibit diverse connectivity, and respond to neuromodulatory inputs like acetylcholine, which promotes invasion and survival.

Trade-off Between Resistance and Persistence in High Cell Density Escherichia Coli Cultures

The paper investigates high cell density physiology in Escherichia coli, revealing a trade-off between resistance and persistence through transcriptional analysis and machine learning.

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

This study uses CRISPR/Cas9 RNP to edit regulatory DNA elements in human islet cells, revealing their role in diabetes risk and β cell function.

Genomic hypomethylation in cell-free DNA predicts responses to checkpoint blockade in lung and breast cancer

This study develops iMethyl, a method to estimate genomic hypomethylation from cell-free DNA to predict responses to immune checkpoint blockade in cancer patients.

iMAX FRET (Information Maximized FRET) for multipoint single-molecule structural analysis

The paper introduces iMAX FRET, a single-molecule method for structural analysis that captures 3D biomolecular structures and conformational changes through FRET and geometrical reconstruction.

Full-Length Single-Molecule Protein Fingerprinting

This paper introduces a method to map amino acid positions and post-translational modifications in single protein molecules with sub-nanometer resolution using FRET-based single-molecule protein fingerprinting.

Data-Driven Strain Design Towards Mitigating Biomanufacturing Stresses

This paper identifies mutations improving stress tolerance in E. coli for melatonin production using a data-driven approach integrating adaptive evolution data and multi-omics analysis.

Rewiring Cancer Drivers to Activate Apoptosis

This paper introduces TCIPs, molecules that activate cell death by rewiring cancer drivers, showing efficacy against DLBCL and potential for broader therapeutic applications.

In vitro broad-spectrum antiviral activity of MIT-001, a mitochondria-targeted reactive oxygen species scavenger, against severe acute respiratory syndrome coronavirus 2 and multiple zoonotic viruses

This study evaluates MIT-001's antiviral activity against SARS-CoV-2 and zoonotic viruses in vitro, showing inhibition of viral replication and restoration of antioxidant gene expression.

Single-cell Electrochemical Aptasensor Array

This paper demonstrates a single-cell electrochemical aptasensor array using nanopillar technology and redox-labelled aptamers for detecting EpCAM-expressing cells, enabling early cancer diagnosis.

De novo fatty-acid synthesis protects invariant NKT cells from cell death, thereby promoting their homeostasis and pathogenic roles in airway hyperresponsiveness

This study shows that de novo fatty-acid synthesis via ACC1 prevents iNKT cell death and promotes their pathogenic roles in allergic asthma.

Identification of hyperosmotic stress-responsive genes in Chinese hamster ovary cells via genome-wide virus-free CRISPR/Cas9 screening

The paper identifies hyperosmotic stress-responsive genes in CHO cells using CRISPR/Cas9 screening and validates their role in stress resistance and protein production.

Silicon as a microfluidic material for imaging and incubation of droplets

This paper demonstrates that silicon chambers improve droplet imaging and incubation by enhancing optical, thermal, and mechanical properties compared to PDMS.

Neural coding of temperature with a DNA-based spiking chemical neuron

This paper introduces a DNA-based chemical neuron that mimics cold temperature sensing by biological neurons through spiking chemical activity and mathematical similarity to a cold nociceptive model.

Amplification of Olfactory Signals by Anoctamin 9 is Essential for Mammalian Olfaction: a Risk Factor for the Covid-19-associated Anosmia

This study identifies Anoctamin 9 as essential for mammalian olfaction, showing its role in amplifying olfactory signals and linking its downregulation to Covid-19-associated anosmia.

Immune and malignant cell phenotypes of ovarian cancer are determined by distinct mutational processes

This study identifies distinct immune and malignant cell phenotypes in ovarian cancer linked to specific mutational processes and anatomic sites.

Course-based Undergraduate Research Module for Enzyme Discovery Using Protein Structure Prediction

This paper describes a course module that teaches protein structure fundamentals through enzyme discovery research, using comparative analysis of protease structures from different organisms.

Data-Driven Strain Design Using Aggregated Adaptive Laboratory Evolution Mutational Data

This paper presents a data-driven workflow to design microbial strains using aggregated ALE mutational data, revealing gene-centric mutations that significantly alter cellular states.

The impact of mutational processes on structural genomic plasticity in cancer cells

The paper analyzes structural genomic variations in cancer cells to understand how mutational processes influence cell-to-cell genomic diversity and tumor evolution.

Visualizing cancer-originated acetate uptake through MCT1 in reactive astrocytes demarcates tumor border and extends survival in glioblastoma patients

This study uses AcePET to visualize tumor boundaries and reactive astrocytes in GBM, showing that targeting MCT1 improves survival by enabling complete resection of cancer stem cells.

α1-COP delivers sphingolipid modifiers and controls plasmodesmal callose deposition in Arabidopsis

The paper identifies α1-COP as a negative regulator of callose accumulation by modulating sphingolipid enzyme translocation in Arabidopsis.

CHRM1 is a Druggable Melanoma Target Whose Endogenous Activity is Determined by Inherited Genetic Variation in DOPA Production

This study identifies CHRM1 as a druggable melanoma target influenced by genetic variation in DOPA production, showing DOPA inhibits CHRM1 to suppress melanoma proliferation.

Encoding multiple virtual signals in DNA barcodes with single-molecule FRET

This study encodes multiple virtual signals in DNA barcodes using single-molecule FRET, enabling the differentiation of six barcodes without sequence exchange.

TRAF6 and TAK1 contribute to SAMHD1-mediated negative regulation of NF-kB signaling

This study identifies TRAF6 and TAK1 as mediators of SAMHD1's suppression of NF-κB activation and HIV-1 infection.

High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

This paper introduces FRET X, a method to measure multiple FRET pairs in single molecules with sub-nanometer resolution by using DNA strand exchange.

Effects of Milk Fat Globule Epidermal Growth Factor VIII On Age-Associated Arterial Elastolysis, Fibrosis, and Calcification

This study investigates how MFG-E8 signaling contributes to age-related arterial damage, showing that its deficiency reduces elastolysis, fibrosis, and calcification in mice.

FRETboard: semi-supervised classification of FRET traces

FRETboard is a semi-supervised tool for classifying FRET traces, improving efficiency and accuracy compared to manual methods and existing software.

Dual mechanism estrogen receptor inhibitors

This paper develops dual-mechanism ER inhibitors that use two distinct targeting moieties to suppress ER activity in breast cancer cells and resistance models.