S. L. Robia

Activation mechanism of the cardiac calcium pump by a small-molecule allosteric modulator

This study uses TCSPC imaging to show that CDN1163, a SERCA2a activator, synergizes with ATP to shift SERCA2a toward a competent conformation, enhancing its function under physiological conditions.

Phospholamban Inhibits the Cardiac Calcium Pump Through Reversing the Allosteric Enhancement of Calcium Affinity by ATP

This paper investigates how phospholamban (PLB) inhibits the cardiac calcium pump SERCA by reversing ATP-induced allosteric enhancement of calcium affinity, using biochemical assays and structural analysis.

RyR2 Binding of an Antiarrhythmic Cyclic Depsipeptide Mapped Using Confocal Fluorescence Lifetime Detection of FRET

The paper maps the binding site of ent-verticilide on RyR2 using FRET and computational methods, identifying two potential loci within the RyR2 structure.

Dilated cardiomyopathy variant R14del increases phospholamban pentamer stability, blunting dynamic regulation of cardiac calcium handling

This study shows that the R14del mutation in phospholamban increases pentamer stability, reducing its ability to regulate SERCA and impairing cardiac calcium handling during physiological stress.

STOICHIOMETRY OF THE SODIUM PUMP-PHOSPHOLEMMAN REGULATORY COMPLEX

The paper investigates the stoichiometry and structure of the NKA-PLM regulatory complex using fluorescence lifetime assays and simulations, revealing a (alpha-beta-PLM)2 composition.

Inhibitory and Stimulatory Micropeptides Preferentially Bind to Different Conformations of the Cardiac Calcium Pump

The paper shows that inhibitory and stimulatory micropeptides bind to different conformations of the cardiac calcium pump SERCA, with their binding preference depending on intracellular Ca2+ levels.

A Kink in DWORF Helical Structure Controls the Activation of the Sarco-plasmic Reticulum Ca2+-ATPase

This study determines the structure of DWORF and shows that a kink caused by Pro15 enables its unique activation of SERCA.

Dwarf open reading frame (DWORF) peptide is a direct activator of the sarcoplasmic reticulum calcium pump SERCA.

This paper identifies DWORF as a direct activator of SERCA, increasing its turnover rate and calcium reuptake, contrasting with the inhibitory effect of phospholamban.

Sarcolambans are phospholamban- and sarcolipin-like regulators of the sarcoplasmic reticulum calcium pump SERCA

The paper investigates sarcolamban peptides from invertebrates to determine their regulatory effects on SERCA1a, finding they act as super-inhibitors with phospholamban-like or sarcolipin-like characteristics.

Dynamics-driven allostery underlies pre-activation of the regulatory Ca2+-ATPase/phospholamban complex

This study uses simulations and experiments to show Ca2+ binding induces structural changes in the SERCA-PLB complex, pre-activating SERCA through dynamic allostery.

Protein Docking and Steered Molecular Dynamics Reveal Alternative Regulatory Sites on the SERCA Calcium Transporter

This study uses SMD and docking to test if PLB interacts with alternative sites on SERCA beyond the canonical site, finding that the canonical site is preferred but alternative sites are favorable under certain conditions.