Y. Shamoo

The biochemical impact of extracting an embedded adenylate kinase domain using circular permutation

This study examines how circular permutation of an adenylate kinase domain affects enzyme activity and stability in mesophilic and thermophilic AKs.

Membrane Lipids Augment Cell Envelope Stress Signaling and Resistance to Antibiotics and Antimicrobial Peptides in Enterococcus faecalis

This study identifies the MadRS system and its operon madEFG as mediators of resistance to antimicrobial peptides and antibiotics in Enterococcus faecalis, linking membrane lipid changes to stress response and virulence.

LiaX is a surrogate marker for cell-envelope stress and daptomycin non-susceptibility in Enterococcus faecium

This study identifies LiaX as a surrogate marker for cell-envelope stress and daptomycin non-susceptibility in Enterococcus faecium and validates its use through ELISA and genomic analysis of clinical isolates.

Molecular Basis of Cell Membrane Adaptation in Daptomycin-Resistant Enterococcus faecalis

This study identifies the role of cardiolipin synthase and the LiaFSR system in daptomycin resistance by redistributing anionic phospholipids in Enterococcus faecalis.

Experimental evolution of Pseudomonas aeruginosa to colistin in spatially confined microdroplets identifies evolutionary trajectories consistent with adaptation in microaerobic lung environments

This study uses microdroplet emulsions to simulate CF lung microenvironments and identifies evolutionary trajectories of Pseudomonas aeruginosa to colistin resistance, showing that PhoP/PhoQ pathway mutations are favored in microaerobic conditions.

Methyl Halide Transferase-Based Gas Reporters for Quantification of Filamentous Bacteria in Microdroplet Emulsions

This paper introduces a methyl halide transferase-based gas reporter system to quantify filamentous bacteria in microdroplet emulsions, achieving higher sensitivity than optical methods.

Evolution of Enterococcus faecium to A Combination of Daptomycin and Fosfomycin Reveals Distinct and Diverse Adaptive Strategies

This study investigates how Enterococcus faecium develops resistance to a combination of daptomycin and fosfomycin, finding that resistance mechanisms are largely independent and that fosfomycin resistance involves changes in phosphoenolpyruvate flux.

Mutational switch-backs can accelerate evolution of Francisella to a combination of ciprofloxacin and doxycycline

This paper shows that using non-interacting drugs ciprofloxacin and doxycycline together can lead to faster evolution of resistance in Francisella compared to using them sequentially.

EfgA is a conserved formaldehyde sensor that halts bacterial translation in response to elevated formaldehyde

This study identifies EfgA as a formaldehyde sensor in Methylorubrum extorquens that inhibits translation under formaldehyde stress, without enzymatic detoxification.

Daptomycin resistance in Enterococcus faecium can be delayed by disruption of the LiaFSR stress response pathway

This study shows that disrupting the LiaFSR pathway in Enterococcus faecium delays daptomycin resistance development, suggesting potential for combinatorial therapy with a LiaFSR inhibitor.

Environment Shapes the Accessible Daptomycin Resistance Mechanisms in Enterococcus faecium

This study shows how different environments shape daptomycin resistance mechanisms in Enterococcus faecium through distinct evolutionary pathways.

The essential role of hypermutation in rapid adaptation to antibiotic stress

This study examines hypermutation's role in Pseudomonas aeruginosa adaptation to colistin, identifying adaptive mutations and highlighting the risk of re-infection from susceptible cells.