Gaudenz M. Danuser

Allosteric Biosensors Unravel GTPase-Effector Feedback

The paper introduces GTPase biosensors that report activity without blocking ligand interactions, revealing feedback mechanisms in Rac1 regulation.

Volumetric Cyclic Immunofluorescence for 3D Spatial Profiling of Tumour, Stroma, and Immune Structures in Human FFPE Tissue

The paper introduces v-CyCIF, a method for 3D spatial profiling of human FFPE tissue, enabling detailed analysis of tumor, stroma, and immune structures.

A genetically encoded RhoG FRET biosensor reveals spatially compartmentalized RhoG-Rac1 signaling during cell protrusion

The paper develops a FRET biosensor to visualize RhoG activity in live cells and shows its spatial relationship with Rac1 during protrusion.

ARHGAP20 organizes spatial Rap1-RhoA signaling coordination controlling adhesion dynamics during migration

This study identifies ARHGAP20 as a regulator of cell migration by modulating Rap1A-RhoA signaling coordination through vesicular trafficking.

RhoG, Rac1 and Cdc42 cooperation in cell protrusion revealed bymultiplexed optogenetics and biosensor imaging

This study uses optogenetics and biosensor imaging to show RhoG activates Rac1 and Cdc42 in cell protrusion, with RhoG's effects on protrusion partially mediated by Rac1 and some independent of it.

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions

This study shows that branched actin-driven membrane protrusions (BAMPs) enhance oncogenic KRAS signaling by amplifying upstream regulators and promoting cell proliferation through merlin inactivation.

Reconstructing Actin Dynamics of the Leading Edge from Observational Data

The paper reconstructs a mechanistic model of actin dynamics at the leading edge of mesenchymal cells using multiplex microscopy data and regression analysis.

In vivo evidence for bleb-induced survival signaling in metastatic melanoma

This paper shows that bleb-induced septin signaling supports survival of metastatic melanoma cells in vivo, and disrupting this pathway reduces metastasis.

Resolving phenotyping discordance with SPACEMAP, an integrated machine learning framework

SPACEMAP is a machine learning framework for multiplex imaging analysis that resolves phenotyping discordance by integrating multiple classification methods.

Universal consensus 3D segmentation of cells from 2D segmented stacks

The paper introduces u-Segment3D, a tool that converts 2D cell segmentations into 3D consensus segmentations without training data, demonstrating effectiveness across diverse datasets.

Phosphoinositide lipids have bidirectional and spatially distinct roles in filopodial dynamics

This study shows phosphoinositide lipids have distinct roles at filopodial tips and bases, affecting actin dynamics and filopodial movement in Xenopus retinal ganglion cells.

Robust statistical assessment of Oncogenotype to Organotropism translation in xenografted zebrafish

This paper develops an imaging-based assay using zebrafish larvae to assess organotropism of cancer xenografts and identifies EWSR1::FLI1 and SOX6 as plasticity factors enhancing metastatic adaptability.

Delta Marches: Generative AI based image synthesis to decode disease-driving morphologic transformations.

Delta-Marches uses generative AI to simulate morphological changes between disease classes, identifying subcellular features that drive disease progression in histopathological images.

Combinatorial responsiveness of single chemosensory neurons to external stimulation of mouse explants revealed by DynamicNeuronTracker

DynamicNeuronTracker enables accurate tracking of neurons in deforming 3D calcium imaging data and reveals combinatorial response profiles of pheromone-sensing neurons to bile acids and sulfated steroids.

Caveolin-1 regulates context-dependent signaling and survival in Ewing Sarcoma.

This study identifies Caveolin-1 as a context-dependent regulator of survival signaling in Ewing Sarcoma, revealing a reversible CD99High state with enhanced survival.

Anchorage-independent cell proliferation promoted by fascin's F-actin bundling

This study identifies fascin-1's role in promoting anchorage-independent proliferation through a novel signaling complex called PASS, which drives Rac1/PAK/JNK signaling in cancer cells.

Cellular Harmonics for the Morphology-invariant Analysis of Molecular Organization at the Cell Surface

The paper introduces u-signal3D, a framework for analyzing molecular organization on cell surfaces in a morphology-invariant way, validated with synthetic and real data.

Surface-guided computing to quantify dynamic interactions between cell morphology and molecular signals in 3D

The paper introduces u-Unwrap3D to map 3D cell surfaces and analyze interactions between geometry and molecular signals, revealing new dynamics in immune and tumor cell behaviors.

Fine-grained, nonlinear image registration of live cell movies reveals spatiotemporal organization of diffuse molecular processes

This paper develops a nonlinear image registration method for live cell movies to analyze spatiotemporal dynamics of diffuse molecular processes, revealing organized Profilin dynamics linked to actin organization.