Lukasz J. Bugaj

Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signaling

This paper uses optogenetic control of RET signaling to induce controlled budding in human kidney organoids.

Optogenetic translocation to subcellular compartments through regulation of protein avidity

This paper introduces Aviatar, a single-component optogenetic system for inducible protein translocation to various subcellular compartments, demonstrating its utility in regulating actin polymerization and cancer-related signaling.

Low-order assemblies drive oncogenic RTK fusion signaling without condensation

This study shows that low-order RTK fusions can drive oncogenic signaling without forming condensates, challenging the role of condensation in signal transmission.

EGFR suppression and drug-induced potentiation are widespread features of oncogenic RTK fusions

The paper shows that RTK fusions suppress EGFR and sequester Grb2, and targeted therapies reverse this suppression, enhancing EGFR signaling and drug response.

Nuclear-import receptors remodel the dilute phase to suppress phase transitions of RNA-binding proteins with prion-like domains.

This study shows nuclear-import receptors suppress RNA-binding protein phase transitions by remodeling the dilute phase, preventing disease-linked aggregation.

Quantifying cancer- and drug-induced changes in Shannon information capacity of RTK signaling

The paper quantifies how cancer and drug treatments affect the information capacity of RTK signaling pathways using optogenetic stimulation and information theory.

Intramolecular competition generates pulsatory protein activity shaped by light, temperature, and evolution

The paper shows that proteins can dynamically encode environmental information through domain interactions, with pulsatory responses shaped by light, temperature, and evolution.

A temperature-inducible protein module for control of mammalian cell fate

This paper introduces a temperature-inducible protein module called Melt that allows reversible control of mammalian cell processes through temperature changes, demonstrating its utility in cell death control in a mouse cancer model.

High-throughput feedback-enabled optogenetic stimulation and spectroscopy in microwell plates

The paper introduces an open-source device for high-throughput optogenetic experiments in microwell plates, enabling automated stimulation, spectroscopy, and feedback control in E. coli.

Optogenetic control reveals differential promoter interpretation of transcription factor nuclear translocation dynamics

The paper introduces CLASP, an optogenetic tool to control TF localization, and shows differential promoter responses to pulsatile vs. continuous TF inputs.