Thomas A. Reh

Long-read single cell transcriptomics uncovers isoform preferences in developing human retina

The paper uses long-read single-cell transcriptomics to study isoform preferences in developing human retina and identifies alternative splicing events that may contribute to cell differentiation.

Stimulating neurogenesis of distinct retinal lineages in human retinal pigmented epithelium (RPE) with proneural transcription factors

The paper identifies NEUROD1 as a factor that, when combined with ASCL1, bFGF, and an Activin A inhibitor, can reprogram human RPE into photoreceptor-like cells.

Identification of transgene insertion sites via short- and long-read whole genome sequencing.

This study presents two whole genome sequencing pipelines to identify and genotype transgene insertion sites in transgenic mice, enabling accurate zygosity determination and breeding strategies.

CUT&TIME captures the history of open chromatin indeveloping neurons

The paper introduces CUT&TIME, a method to map historical chromatin accessibility in single cells during neurogenesis, revealing early changes in promoter accessibility linked to cell-fate commitment in retinal ganglion cells.

Dynamic expression of ASCL1 drives neurogenesis from infant and adult macaque Müller glia into immature retinal ganglion cells

This study shows that ASCL1 can reprogram adult and infant macaque Müller glia into retinal ganglion cell-like neurons using a modified vector cassette.

Ciliary marginal zone of the developing human retina maintains retinal progenitor cells until late gestational stages

The paper identifies a proliferative region in the fetal human retina that continues to generate neurons late in development, similar to the non-mammalian ciliary marginal zone.

Synchronized long-read genome, methylome, epigenome, and transcriptome for resolving a Mendelian condition

Pooling Fiber-seq gDNA with concatenated MAS-Seq cDNA in one PacBio run gives phased genome, methylome, chromatin, and transcriptome, exposing four gene disruptions from one X;13 translocation.

Stimulating the regenerative capacity of the human retina with proneural transcription factors in 3D cultures

This study shows human Muller glia can generate new neurons in adults using proneural transcription factors in 3D cultures.

A multiplexed, single-cell sequencing screen identifies compounds that increase neurogenic reprogramming of murine Muller glia.

AAV-mediated expression of proneural factors stimulates neurogenesis from adult Müller glia in vivo.

This study shows that AAV-mediated delivery of proneural factors to adult Müller glia stimulates neurogenesis in vivo, producing neurons similar to those in the retina.

Single-cell sequencing of individual retinal organoids reveals determinants of cell fate heterogeneity

The paper uses sci-Plex to analyze retinal organoids and identifies that early Wnt signaling increases retinal cell classes.

Cell fate specification and conversion generate foveolar cone subtype patterning in human retinal organoids

The paper identifies mechanisms that generate M/L cone dominance and suppress S cones in the human foveola using retinal organoids and signaling pathway regulators.

NFkB-signaling suppresses Müller glia-mediated neuron regeneration in the mammalian retina

This study shows that NFkB signaling suppresses Müller glia-mediated neuron regeneration in the mammalian retina by promoting immune cell accumulation and inhibiting neurogenic potential.

Spatiotemporal specification of human green and red cones

The paper investigates the spatiotemporal development of M and L cones in the human retina, finding that retinoic acid signaling promotes M cone fate early in development.

Cis-Regulatory Accessibility Directs Muller Glial Development and Regenerative Capacity

The paper identifies loss of neurogenic gene expression and motif accessibility in mature Muller glia that limits their regenerative capacity.

STAT pathway activation limits the Ascl1-mediated chromatin remodeling required for neural regeneration from Muller glia in adult mouse retina.

This study shows that STAT pathway activation limits Ascl1-mediated chromatin remodeling in Muller glia, reducing their ability to regenerate neurons in the adult mouse retina.