Franck Perez

Non-covalent reversibly photoconvertible fluorescent tags for wash-free protein labeling

The paper introduces non-covalent reversibly photoconvertible fluorescent proteins that change color under illumination, enabling discrimination of spectrally similar variants in live cells.

Kinetic and Mechanistic Characterization of RSpFAST, A Non-Covalent Versatile Chemogenetic Platform for Fluorescence Imaging

This paper introduces RSpFAST, a non-covalent chemogenetic platform for fluorescence imaging, and demonstrates its dual behavior as a stable label and a reversible photoswitcher.

Chemically responsive protein switches for the precise control of biological activities

This paper introduces CATCHFIRE and CATCH-ON systems for chemically induced protein dimerization to control biological activities with precision and reversibility.

Mechanical forces stimulate Golgi export

The paper shows extracellular mechanical forces influence Golgi secretory function by modulating carrier biogenesis and exocytosis, with molecular determinants including microtubule acetylation and protein kinase D activity.

Optimization of the VSV G backbone for amino terminal fusion with nanobodies allowing its retargeting to receptors of therapeutic interest.

The paper identifies mutations in the VSV G glycoprotein that improve its functionality when fused with nanobodies, enabling specific retargeting to therapeutic receptors.

De novo Golgi biogenesis requires coordinated transactivation of a Golgi regulon

The paper identifies a Golgi regulon and shows CREB3L1 drives its transcriptional activation during de novo Golgi biogenesis.

A tunable and versatile chemogenetic near infrared fluorescent reporter

The paper introduces nirFAST, a tunable NIR fluorescent reporter for multiplexed imaging, enabling subdiffraction resolution and cell cycle monitoring in live cells.

Cell Painting transfer increases screening hit rate

The paper uses Cell Painting images to preselect compounds for drug screening, significantly increasing hit rates by reducing the number of compounds tested.

Differential screening identifies molecules specifically inhibiting CCR5 transport to the cell surface and HIV infection

The paper identifies molecules that inhibit CCR5 transport to the cell surface and HIV infection by targeting palmitoylation.

Visualizing Wnt secretion from endoplasmic reticulum to filopodia

The paper uses the RUSH system to visualize Wnt secretion from the endoplasmic reticulum to filopodia, showing WLS is rate-limiting for ER exit and filopodia enhance Wnt signaling.