Sperm and Testicular Function

The paper shows VCP downregulates mono-ubiquitinated H2A to support spermatocyte differentiation in Drosophila, acting downstream of tTAFs.

The paper compares spermatogenic gene expression in opossum and mouse to identify evolutionary dynamics in germline biology.

This study shows that the X-linked miR-506 family miRNAs enhance sperm competitiveness and male reproductive fitness by fine-tuning gene expression during spermatogenesis.

This study identifies Mob4 as essential for male fertility in Drosophila by regulating spermatid individualization and axonemal structure.

The paper identifies widespread extrachromosomal circular DNA (eccDNA) formation during spermatogenesis and shows microhomology-mediated end joining is the major biogenesis mechanism.

This study uses scRNA-seq to show that DDX43 is essential for chromatin remodeling during spermiogenesis, with its deficiency causing defects in sperm nucleus condensation.

This study shows that DOT1L regulates chromatin reorganization and gene expression in male germ cells, leading to fertility defects in its absence.

The paper uses scATAC-Seq to analyze chromatin accessibility in mouse developing testis, identifying regulatory elements, transcription factors, and GWAS-associated targets.

This study uses single-cell RNA sequencing to show that CTCF depletion causes transcriptional defects during spermiogenesis, leading to sperm abnormalities and infertility.

This study shows that SSH2 is essential for acrosome biogenesis and male fertility in mice by regulating actin remodeling through COFILIN.

The paper shows that Cre recombinase can be transported from somatic tissues to the male reproductive tract in mice, suggesting inter-tissue RNA or protein trafficking.

This study identifies spastin's essential role in regulating microtubule dynamics during spermatogenesis, revealing its critical impact on male gamete development and function.

This study identifies bi-allelic CCDC146 mutations as a cause of non-syndromic male infertility in humans and mice, showing its role in sperm flagellum structure and microtubule organization.

This study identifies Tex10 as a regulator of Wnt signaling in PGC specification and male germline development using mouse models and single-cell RNA sequencing.

The paper shows that MEK1/2 signaling is essential for male fetal germline development in mice, while FGF signaling has a limited role.

This study shows that eIF4E-5 is essential for spermatid cyst polarization, individualization, and male fertility in Drosophila by regulating caspase activity and Soti localization.

This study identifies genetic subforms of non-obstructive azoospermia using exome sequencing and single-cell RNA sequencing, revealing molecular groups of genes involved in spermatogenesis.

This study uses single-cell RNA sequencing to analyze testicular aging in humans, identifying molecular mechanisms and potential therapeutic targets for age-related male fertility decline.

The paper analyzes transcriptomes of human testis with different germ cell arrest stages to identify gene and transcript dynamics during spermatogenesis.

This study examines steroidogenesis and signaling in in vitro matured prepubertal mouse testicular tissues, finding altered hormone levels and gene expression, suggesting limitations in current culture systems.

This study investigates the role of Tcte1 mutations in male infertility using mouse models and human samples, finding haploinsufficiency and disrupted sperm function.

This study shows that CFTR deficiency in mice causes testicular microenvironment imbalance, leading to over-activated caspase signaling and infertility.

This paper identifies ADAD2 and RNF17 as interacting proteins forming novel meiotic germ cell granules essential for male fertility, revealing their distinct morphological subtypes and functional roles.

This study shows that SRSF10 regulates alternative splicing in progenitor spermatogonia, essential for their expansion and spermatogenesis.

This study shows that male Padi2/Padi4 double knockout mice exhibit delayed puberty, reduced fertility, and lower testosterone levels, linking PADs to hormone signaling in vivo.

This study shows that absence of estrogen in male rabbits leads to reduced fertility, lower sperm count, and increased flagellar defects in sperm.

This study shows human TMEM95 binds eggs, identifies a binding region via crystallography, and develops antibodies that impair sperm-egg fusion without blocking binding.

The paper identifies distinct gene regulation by NHR-23 and SPE-44 during C. elegans spermatogenesis and shows their combined depletion affects additional genes.

This study shows SRSF2 is essential for mRNA splicing and spermatogenesis, with its deletion causing infertility and disrupting key developmental processes.