Sperm and Testicular Function

The paper optimizes sperm processing and cryopreservation methods in common marmosets to improve motility and reduce DNA fragmentation for IVF applications.

This study identifies the Drosophila TENT5 poly(A) polymerase, Isep, as essential for spermatid elongation and individualization, with its function independent of poly(A) polymerase activity.

The paper identifies misregulated sperm components in mice with a selfish supergene using single-nucleus transcriptomics and phosphoproteomics, revealing dysregulated phosphorylation and gene expression related to sperm motility.

This study shows that Gnpat-knockout mice exhibit testicular interstitial remodeling and germ-cell loss due to ether-lipid deficiency, with no DNA-damage checkpoint activation.

This study compares five DNA extraction protocols from goat semen, finding that Modified Protocol 2 provides the highest DNA yield, purity, and PCR success.

The paper introduces HSMV, a dataset of human sperm microscopy videos for detection, tracking, and motility analysis, with annotated and unannotated clips for algorithm benchmarking.

This study compares mitochondrial and metabolic properties of stem and progenitor cells in the male germline, finding that stem cells exhibit metabolic plasticity and latent oxidative capacity.

This study shows adenosine promotes spermatogonial differentiation and regulates the balance between proliferation and differentiation in mice.

This study shows that mitochondrial calcium uptake via CatSper and MCU is essential for hyperactivated motility during human sperm capacitation, similar to mouse sperm.

The paper identifies genetic interactions of the phosphatidylinositol phosphate signaling pathway in Drosophila spermatogenesis through a sensitized genetic screen.

This study identifies calcium-activated chloride channels (CaCCs) as key regulators of sperm capacitation, showing their greater impact compared to voltage-gated chloride channels.

This study identifies TMC5 as essential for mechanoresponsive calcium entry during spermiogenesis, linking mechanical cues to sperm development and male infertility.

The paper shows that paternal diet affects both paternal and maternal transcriptomes in early embryos through distinct sperm-related mechanisms.

This study maps the human sperm proteome across compartments, donors, and individual cells, revealing conserved and variable proteins and linking mitochondrial dysfunction to male infertility.

The paper identifies the chromatoid body as a dynamic RNA sorting center in spermatogenesis, revealing its role in RNA surveillance and transcript regulation through sequence and structural features.

This study identifies LRRC37A as critical for acrosomal ion homeostasis, showing its loss leads to male infertility and acrosomal enlargement.

This study examines the effects of glycosylation and deglycosylation disorders on zebrafish sperm quality, showing reduced concentration, motility, and membrane integrity in mutant fish.

Single-nucleus RNA+ATAC profiling of 32 mice shows asymmetric PRDM9 binding drives asynapsis, pachytene cell loss, aneuploidy and multi-megabase crossover redistribution, tuned by a chromosome 15 locus.

This study investigates how surface N-acetylglucosamine (GlcNAc) changes in bovine sperm during epididymal maturation and its role in binding to oviductal epithelial cells.

This study creates a single-nucleus multiomic and spatial atlas of human spermatogenesis, linking gene regulatory networks to infertility-associated genetic variation and disease perturbations.

This study investigates age-related changes in male reproductive capacity in Anopheles culicifacies, identifying AcLITAF6 as a key regulator of sperm quality and male fertility.

This study evaluates how curcumin and penicillin-streptomycin affect the diversity and structure of the chicken semen microbiome using 16S sequencing.

This study shows early-life stress alters the transcriptome and chromatin accessibility of mouse spermatogonial cells, with molecular signatures persisting into adulthood.

This study shows that trehalose improves cryopreservation of piglet testicular tissue by enhancing mitochondrial function and reducing oxidative stress.

This study identifies soluble ZP2 N-terminal fragments as activators of CatSper-dependent Ca2+ entry, regulating sperm motility and acrosomal exocytosis in mice and humans.

This study shows mammalian sperm use cytoplasmic droplets and mitochondria for protein synthesis during epididymal maturation, overcoming transcriptional silence to achieve motility and fertility.

This study investigates ferroptosis in Leydig cells, showing it disrupts steroidogenesis and reveals hormone-mediated protection mechanisms.

This study shows that paternal metabolic recovery before conception remodels sperm RNA and reduces transmission of HFD-associated metabolic risk to offspring in mice.

This study identifies molecular factors shaping small RNA content in mouse sperm, revealing dynamics and protein associations during spermiogenesis and capacitation.