Bacterial Genetics and Biotechnology

This study identifies DNA binding by type IV pili as a rate-limiting step in natural transformation, showing that enhancing DNA binding in Acinetobacter pathogens increases transformation rates by 100-fold.

The paper shows that the archaeal transcription factor RosR has divergent functions across species despite strong sequence conservation, likely due to flexible cis-trans interactions.

This study shows that transposable elements in E. coli can evolve de-novo promoter activity through mutations, highlighting their latent cis-regulatory potential.

The paper identifies novel T11SS cargo proteins across proteobacteria using genomic co-occurrence analysis and validates one novel cargo type experimentally.

This study identifies the CarSR two-component system as a direct regulator of radD in Fusobacterium nucleatum, revealing its role in sensing coaggregation and regulating adhesion.

The paper shows that basal bodies in Bacillus subtilis are mobile during early assembly and become immobilized when the rod transits the peptidoglycan, suggesting rod polymerization probes the peptidoglycan for pores.

The paper identifies two natural isolates of Agrobacterium tumefaciens with fused chromosomes, revealing constraints for replicon fusion and survival.

The paper uses Bayesian inference to predict antibiotic survival in E. coli by integrating gene expression and growth rate data from single cells.

This paper identifies SigAb as a metal stress-responsive ECF σ factor in Acinetobacter baumannii, showing it directly regulates a small set of genes and indirectly controls hundreds involved in metal resistance and fitness.

This study identifies novel targets of DtxR and HrrA in Corynebacterium glutamicum and reveals their interconnected regulatory networks under varying iron and heme conditions.

The paper investigates how premature terminations and internal promoters affect operon responses during genome-wide stresses in bacteria.

The paper identifies and maps Tn916 insertion sites in Bacillus subtilis chromosome, revealing a consensus motif and a hotspot region.

This study identifies how the ChvG-ChvI pathway in Agrobacterium tumefaciens promotes survival under cell wall stress through exopolysaccharide production, beta-lactamase activity, and outer membrane remodeling.

This study identifies a temperature-sensitive regulatory mechanism in Staphylococcus aureus involving an RNA and ClpXP protease that controls cell division hydrolases.

The paper identifies an antisense RNA, ArrA, that inhibits DnaA production in Bacillus subtilis and affects sporulation timing.

This study shows that ciprofloxacin induces DNA supercompaction in E. coli, requiring RecN and dependent on RecA, as part of a DNA damage response.

This study shows that cyanoexosortase B (CrtB) is essential for motility, biofilm formation, and scytonemin production in Nostoc punctiforme.

This study links DnaA activity oscillations to DNA replication initiation in E. coli, showing they persist independently of dnaA expression and proposing an extrusion model for regulation.

This study combines live cell fluorescence imaging and in situ cryo electron tomography to investigate the septation process in Deinococcus radiodurans, revealing insights into cell envelope architecture and molecular mechanisms of septal growth and closure.

This study shows Pseudomonas aeruginosa chemotactically responds to serotonin, dopamine, epinephrine, and norepinephrine, with TlpQ chemoreceptor involvement, and highlights their role in virulence.

This study identifies SanA as an inner membrane protein in Salmonella that influences antibiotic resistance and infection phenotype by regulating SPI-I expression during growth phases and host cell entry.

The paper develops a biophysical model to elucidate how Spo0A[~]P binding to its regulatory boxes affects spo0A transcription in Bacillus subtilis, revealing repressive effects and the role of RNAP holoenzyme levels in expression increase.

This paper investigates how DNA binding proteins evolved sequence specificity by analyzing the IHF/HU family and reconstructing ancestral sequences.

This study uses Tn-seq to identify metal resistance genes in Pseudomonas putida, revealing both known and novel genes involved in cadmium, copper, and cobalt resistance.

This study uses RNA-sequencing to analyze E. coli's transcriptional response to hypothiocyanite, identifying rclB and rclC as potential regulators of membrane permeability.

The paper identifies cis-regulatory elements and transcription factors regulating the cps locus in Neisseria meningitidis, revealing the role of HTH_XRE in promoter function.

This study elucidates the mechanism of MBOAT-SGNH acyltransferases in Gram-negative bacteria, showing that a tyrosine-containing peptide shuttles acyl groups from MBOAT to SGNH active sites for peptidoglycan O-acetylation.

This paper investigates the role of teichoic acids in maintaining the periplasmic space in Streptococcus pneumoniae using cryo-electron microscopy and metabolic labeling, revealing new insights into TA composition and function.

This study investigates the unique signaling mechanisms of the RT-HK kinase in the GSR network of Rubellimicrobium thermophilum, revealing inverted logic and specific downstream interactions.

This study investigates the role of HU in chromosome organization and replication in Streptococcus pneumoniae, showing it promotes long-range interactions and supports replication and segregation.