Bacterial Genetics and Biotechnology

The paper quantifies fitness costs of protein overexpression in E. coli, showing growth collapse due to membrane proteome displacement, not translocation pathway saturation.

The paper reports cryo-EM structures of P. aeruginosa PA3214 and E. coli PqiC lipoproteins interacting with their MCE protein partners, revealing their assembly and functional interactions.

The paper identifies outer membrane stiffness as a determinant of multidrug tolerance in E. coli by showing it limits antibiotic-induced envelope destabilization.

This study identifies TpeX as a pore-forming T6SS effector in P. aeruginosa that disrupts bacterial membranes, with its activity regulated by an immunity protein.

The paper performs a cross-study transcriptomic meta-analysis of E. coli K-12 ALE experiments to identify conserved adaptive gene expression patterns across diverse conditions.

This study deletes nine NAPs from E. coli to create a strain lacking native chromatin, revealing gene expression homogenization and demonstrating that bacterial life can proceed without chromatin proteins.

This study identifies antibiotic-specific pathways governing bacterial persistence in E. coli, showing persistence is largely independent of growth and depends on distinct physiological mechanisms for different antibiotics.

The paper shows that promoters and terminators in bacteria are often coupled, with termination sequences contributing to promoter function, leading to regulatory interference between adjacent transcription units.

This paper shows that loss of YqeK in S. aureus increases stress sensitivity and reduces virulence gene expression, suggesting YqeK as a target for anti-virulence drugs.

The paper demonstrates that synthetic arginine-rich motif peptides can disrupt sRNA function by interfering with Hfq binding, specifically targeting MicF in E. coli.

This study uses transposon mutagenesis in E. coli to identify genes affecting streptomycin susceptibility, revealing SbmA's role in aminoglycoside uptake.

This study quantifies the cost-benefit trade-off of peritrichous flagellation in Salmonella enterica, showing that increased flagellation reduces growth rate but enhances motility and competitive fitness in structured environments.

The paper shows small plasmids can cause antibiotic heteroresistance through copy number increases via plasmid mutations or chromosomal recD mutations in Escherichia coli.

Live E. coli gyrase clusters hold excess GyrB (B:A 2.5:1), show hexanediol-sensitive liquid-like turnover, and docking suggests GyrB bridges heterotetramers.

This paper determines the cryo-EM structure of CbrA, revealing its SLC5-STAC domains and how CbrX interacts with the transporter, providing insights into histidine transport and signaling mechanisms.

This study investigates how DrHU in Deinococcus radiodurans organizes nucleoid structure through two DNA binding modes, leading to distinct 2D and 3D arrangements under different conditions.

This study identifies PtrA as a periplasmic regulator that controls carbapenem entry in Pseudomonas aeruginosa through adaptive impermeability, independent of OprD abundance and transcriptional regulation.

This study investigates how gene loss affects bacterial fitness in E. coli by analyzing proteomic data and identifying gene categories with varying fitness impacts.

The paper identifies key molecular steps in the MreB polymerization cycle through genetic screening and biochemical analysis in Bacillus subtilis.

This study shows that CetZ1 is essential for the polar assembly of the archaeal motility machinery, with distinct roles from its paralog CetZ2.

This study identifies AstR as a regulator of ornithine catabolism in Acinetobacter baumannii, essential for gut colonization by enabling competition with the microbiota.

The paper identifies a fluorescent membrane reporter, PtsG-GFP, for dynamic cell cycle analysis in Bacillus subtilis and demonstrates its utility in tracking cytokinesis.

The paper introduces an in vitro transcription assay using a Broccoli RNA aptamer to test bacterial RNA polymerases, promoters, and inhibitors, showing its utility for drug discovery.

The paper identifies an mRNA-binding motif recognized by the intrinsically disordered AR2 domain of RNase E, showing its role in substrate recognition and autoregulation.

The paper confirms high levels of antisense transcription in Orientia tsutsugamushi across multiple strains and suggests it may be a regulated mechanism in its lifecycle.

This paper identifies PilU as a second T4P retraction motor in M. xanthus, showing it is essential for force generation and modulated by calcium and a flexible C-terminal region.

The paper uses machine learning to show that host chromosomal gene content predicts plasmid biology traits like carriage status and mobility potential across prokaryotic genomes.

This study develops assays to monitor single-cell bioenergetic status and cell lysis in Bacillus subtilis cultures during differentiation and growth.

This paper develops a standardized toolkit for predictable and tunable protein secretion and surface display in Bacteroides thetaiotaomicron by optimizing lipoprotein signal peptides.

The paper identifies a mechanism where a small RNA guides a protein regulator to target mRNAs, enabling programmable post-transcriptional control in bacteria.