Bacterial biofilms and quorum sensing

This study identifies genes essential for Pseudomonas aeruginosa aggregate formation in CF sputum using RNA-seq and microscopy, revealing distinct spatial phenotypes and cooperative interactions.

This study shows that serotype switching in Pseudomonas aeruginosa ST111 from O4 to O12 enhances adhesion, motility, and virulence in murine pneumonia.

This study uses far-red fluorogenic probes to reveal single-cell dispersal patterns in Vibrio cholerae biofilms, showing that dispersal starts at the periphery and 25% of cells never disperse.

This study develops BaSSSh-seq, a bacterial single-cell RNA sequencing method, to analyze transcriptional heterogeneity and immune responses in biofilms.

This study uses expansion microscopy to differentiate adhesive interactions between microbial cells in biofilms at the single-cell level.

The paper shows that bacterial quorum sensing optimizes carbon metabolism by regulating methionine and tetrahydrofolate synthesis, enhancing growth in changing glucose conditions.

This study analyzes RNA sequencing data on Staphylococcus aureus biofilm formation using machine learning and co-expression analysis to identify functional modules and novel protein interactions, and develops a database for storing and analyzing the data.

The paper determines six structures of DosP, revealing how its heme-binding PAS domain regulates c-di-GMP phosphodiesterase activity through allosteric mechanisms.

The paper identifies vca0646-0649 genes regulated by HapR that induce cell death in Vibrio cholerae independently of the T6SS system, revealing a second parallel cell death pathway.

This study quantifies how E. coli biofilm channel complexity changes with cell shape and growth conditions using fractal analysis.

This study quantifies how interspecies interactions affect antibiotic sensitivity of Pseudomonas aeruginosa in polymicrobial infections and shows these interactions can alter treatment effectiveness.

The paper analyzes 1D aggregation models of bacterial populations with non-exponential reversal times, showing they improve aggregate formation and persistence.

This study investigates how Pseudomonas aeruginosa adapts to the airway environment by balancing biofilm formation for colonization and antibiotic tolerance, revealing trade-offs that contribute to chronic infections.

The paper shows E. coli cells enter phase-separated extracellular polymeric substances containing HU, DNA, and LPS, with LPS modulating liquidity and structural integrity of biofilms.

This study shows free-floating bacteria detect shear flow to alter gene expression, enhancing survival in low oxygen conditions through quorum sensing and oxygen gradient formation.

This study shows Pseudomonas aeruginosa kills Staphylococcus aureus via polyphosphate-dependent mechanisms, primarily through polyP-regulated pyocyanin production.

This study uses microscopy to examine interactions between Pseudomonas aeruginosa and six opportunistic pathogens, revealing a range of ecological interactions from mutualism to antagonism.

This study investigates the early aggregation dynamics and formation of diverse cellular structures in Myxococcus xanthus under nutrient-poor and low density conditions.

This study shows low-dose antibiotics trigger bacterial aggregation via lysis and extracellular DNA release, forming aggregates distinct from biofilms.

This paper clarifies the role of Prc protease in mucoid conversion of Pseudomonas aeruginosa by showing it directly degrades MucA22, a truncated form of MucA, and not wild-type MucA.

This study shows that Pseudomonas aeruginosa uses surfactants from other species as public goods to enable surface motility, allowing it to move in diverse environments.

This study investigates the role of pqsR and pqsE in the QS regulon of P. aeruginosa, finding limited influence of PqsR and conditional influence of PqsE on gene activation.

This study identifies a phosphorelay pathway, HmrABCX, that regulates the transition from motile to sessile lifestyle in Caulobacter crescentus independently of HfiA, and shows its role in environmental stress responses.

This study investigates the anti-pathogenic effects of the polyherbal formulation Enteropan against multidrug-resistant Pseudomonas aeruginosa, identifying molecular mechanisms and potential drug targets.

This study identifies distinct biofilm formation mechanisms in multiple Staphylococcus species, showing independent evolution of biofilm pathways through different protein domains.

This study investigates the catalytic mechanisms and substrate specificity of the lactonase GcL, revealing its promiscuity in reaction mechanisms and broad substrate acceptance.

This paper investigates the regulation and role of L-lactate dehydrogenases LldD and LldA in Pseudomonas aeruginosa, showing they are conditionally expressed and contribute to survival during macrophage infection.

This study shows that biased dispersal of a dominant bacterial species prevents competitive exclusion, enabling coexistence in multispecies biofilms.

The paper identifies a toxin-antitoxin system in biofilms where c-di-GMP regulates genome stability and antibiotic persistence by controlling HipH activity.

This study shows that Pseudomonas aeruginosa uses type IV pili-mediated chemotaxis to invade and disrupt Staphylococcus aureus microcolonies, enhancing interspecies antagonism.