CRISPR and Genetic Engineering

This study evaluates the functional impact of genetic variants using a high-throughput prime editing sensor strategy, identifying both known and novel TP53 variants with diverse effects on p53 function.

This study uses long-read sequencing to accurately identify correct alleles in genome-edited animals despite mosaicism and complex edits.

The paper investigates how specific mutations in the Rec3 domain of CRISPR-Cas9 variants affect structural dynamics and allosteric communication to enhance specificity.

This paper describes an engineered E. coli strain that produces long single-stranded DNA efficiently, validated for use in genome editing and DNA origami.

The paper describes a method to assemble small synthetic genes with repetitive elements using Golden Gate Assembly, achieving high fidelity for challenging gene constructs.

The paper designs mutated pRN1 plasmid backbones to evade CRISPR immunity in Saccharolobus islandicus, enabling stable plasmid vectors and a dual plasmid system for genetic studies.

The paper introduces a modular CRISPR vector assembly system called Fragmid, enabling rapid creation and testing of CRISPR technologies through combinatorial design and pooled screening.

This study compares robotic and manual injection methods for CRISPR-CasRx RNA knockdown in zebrafish embryos, finding similar efficiency and phenotype rates between approaches.

The paper shows anti-CRISPR proteins in S. pyogenes reduce Cas9 autorepression, leading to increased CRISPR-Cas expression and enhanced phage defense.

The paper improves gene editing in Leishmania by combining AsCas12a ultra, T7 RNA Polymerase, and a cytosine base editor, enabling efficient functional screening of genes.

This study identifies Csx23 as a CRISPR effector that uses cyclic nucleotide binding to mediate antiphage defence in Vibrio cholerae.

This study engineers self-deliverable CRISPR ribonucleoproteins for efficient genome editing in neural progenitor cells and mouse brain tissue.

This paper introduces CRISTAL, a platform for programmable RNA regulation using inducible Cas13 circuits, enabling precise temporal control and multiplexed gene regulation in mammalian cells.

This paper develops a CRISPR interference system in Azotobacter vinelandii to silence nitrogen fixation genes and demonstrates its utility for combinatorial gene expression studies.

The paper describes a method for efficiently constructing genes from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate Assembly.

This study uses protein language models to engineer a uracil-N-glycosylase variant for programmable T-to-G and T-to-C base editing, achieving high success rates in correcting genetic mutations in mice.

The paper adapts Cas9-based gene editing to study species-specific competition mechanisms in Klebsiella species.

This paper describes the directed evolution of lambda integrase variants that target microalgal and human genome sequences, showing enhanced specificity for non-natural sites.

CRISPR-COPIES identifies neutral integration sites for CRISPR/Cas-mediated gene insertion in bacterial, fungal, and human genomes using computational methods.

This paper describes a method to generate CRISPR-edited birch plants without DNA integration using Agrobacterium-mediated transformation, achieving an 80% mutation rate and identifying 7.69% DNA-free edited lines.

This study develops an endogenous CRISPR-Cas system for gene editing in Bifidobacterium breve, enabling deletion, substitution, and continuous editing of target genes.

This study uses genome editing to improve Hassawi rice traits for sustainable cultivation, resulting in shorter growth cycles, increased productivity, and better nutritional attributes.

The paper identifies and characterizes CoCoNuTs, a new subclass of Type IV restriction systems that target RNA, with additional potential DNA targeting capabilities and regulatory mechanisms.

The paper investigates a gene drive targeting the wupA gene in Drosophila to skew sex ratios, finding it can reduce female progeny and is effective across multiple species.

This study investigates how RNA structure affects Cas13 activity, revealing two mechanisms by which secondary structure modulates Cas13 and improving RNA detection through strand displacement.

The paper uses CRISPRi in Bacteroides thetaiotaomicron to identify a small RNA that modulates bile salt susceptibility.

The paper presents a scalable CRISPR-Cas9 platform for chemical-genetic screens of DNA damage-inducing compounds, identifying enriched modes-of-action and revealing late ICL repair pathway responses.

This paper identifies and engineers a novel Cas12a nuclease (AiEvo2) with improved specificity and efficacy for human gene editing, including applications in Huntington's disease and CAR-T therapy.

This study investigates the interference requirements of Type III-A and III-B CRISPR-Cas systems from Thermus thermophilus, finding that target RNA abundance influences crRNA-target complementarity needed for interference.