C. J. Kuo

Engineered extracellular matrices reveal stiffness-mediated chemoresistance in patient-derived pancreatic cancer organoids

This study shows that high stiffness engineered matrices induce chemoresistance in pancreatic cancer organoids through hyaluronan mechanosignaling and a plastic cancer stem cell phenotype.

Deterministic evolution and stringent selection during pre-neoplasia

This study models pre-neoplasia in human gastric organoids by inactivating TP53, revealing progression to malignant transcriptional programs and clonal dynamics through experimental evolution.

A focal adhesion kinase-YAP signaling axis drives drug tolerant persister cells and residual disease in lung cancer

This study identifies a FAK-YAP signaling axis that drives drug-tolerant persister cells and residual disease in lung cancer, showing that inhibiting this axis improves tumor response to targeted therapies.

Pan-cancer organoid validation of tumor outlier chromosomal amplification events

The paper validates oncogenic chromosomal amplification events across multiple cancers using organoid models and functional assays.

Targeted replacement of full-length CFTR in human airway stem cells by CRISPR/Cas9 for pan-mutation correction in the endogenous locus

This study corrects multiple CFTR mutations in human airway stem cells using CRISPR/Cas9 and AAV vectors, achieving functional restoration in differentiated epithelial cells.

Progenitor identification and SARS-CoV-2 infection in long-term human distal lung organoid cultures

This paper develops a feeder-free organoid culture system for human distal lung progenitors and demonstrates SARS-CoV-2 infection of club cells in a novel in vitro model.

Highly Efficient Repair of the ΔF508 Mutation in Airway Stem Cells of Cystic Fibrosis Patients with Functional Rescue of the Differentiated Epithelia

This paper corrects the ΔF508 mutation in CF patient airway stem cells using CRISPR-Cas9 and AAV6, achieving high correction rates and functional rescue in differentiated epithelia.