S. H. Randell

Prolonged airway explant culture enables study of health, disease, and viral pathogenesis

This study develops methods to culture human and animal airway explants for at least 14 days, preserving tissue architecture and enabling study of health, disease, and viral infection.

Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy

This study shows that viral airway injury enhances cell engraftment in an in vitro CF model by creating conditions that favor non-CF cell integration and functional CFTR recovery.

A Unique Cellular Organization of Human Distal Airways and Its Disarray in Chronic Obstructive Pulmonary Disease

This study identifies a unique epithelial cell population in human distal airways and shows its reduction in COPD, linked to IFN-gamma signaling.

Visible blue light inactivates SARS-CoV-2 variants and inhibits Delta replication in differentiated human airway epithelia

This study shows visible blue light inactivates SARS-CoV-2 variants and inhibits Delta replication in human airway models, with high efficacy and tolerance.

Robust W1282X-CFTR rescue by a small molecule GSPT1 degrader

The paper develops Bmi-1/hTERT cell lines to model CFTR variants and shows that CC-90009 partially rescues W1282X-CFTR function while affecting sodium channel activity.

Hyperconcentrated Mucus Unifies Submucosal Gland and Superficial Airway Dysfunction in Cystic Fibrosis

This study identifies hyperconcentrated mucus as a unifying mechanism for submucosal gland and superficial airway dysfunction in cystic fibrosis.

A molecular atlas of proximal airway identifies subsets of known airway cell types revealing details of the unique molecular pathogenesis of Cystic Fibrosis

The paper uses single-cell transcriptomics to analyze proximal airway epithelium in CF and healthy donors, identifying molecular changes linked to CF pathogenesis.

Targeted replacement of full-length CFTR in human airway stem cells by CRISPR/Cas9 for pan-mutation correction in the endogenous locus

This study corrects multiple CFTR mutations in human airway stem cells using CRISPR/Cas9 and AAV vectors, achieving functional restoration in differentiated epithelial cells.

DNAJB12 and Hsp70 Facilitate the Conformation Specific Degradation of Arrested N1303K-CFTR Intermediates by ERQC-Autophagy

This study shows DNAJB12 and Hsp70 facilitate conformation-specific degradation of N1303K-CFTR intermediates via ERQC-autophagy, distinct from ERAD.

SARS-CoV-2 D614G Variant Exhibits Enhanced Replication ex vivo and Earlier Transmission in vivo

This study shows the SARS-CoV-2 D614G variant replicates more efficiently and transmits faster in hamsters than the wildtype virus.

Single cell reconstruction of human basal cell diversity in normal and IPF lung.

The paper uses single-cell RNA sequencing to analyze basal cell diversity in normal and IPF lung, finding increased secretory primed basal cells in IPF and roles for NOTCH2 and NOTCH3 in their regulation.

Trypsin treatment unlocks barrier for zoonotic coronaviruses infection

This study shows that proteolytic cleavage, not receptor binding, is the main barrier for two MERS-like bat coronaviruses to infect human cells, and that trypsin treatment can overcome this barrier.