The paper introduces a HILIC-MS/MS method to analyze acyl-CoA and acyl-carnitine species, revealing differences in fatty acid oxidation and cellular energy status across cell lines.

bioRxiv · Aug 17, 2026 · PreprintBiochemistry

A Ketogenic Diet Sensitizes Pancreatic Cancer to Inhibition of Glutamine Metabolism

Omid Hajihassani, Asael Roichman, Jacob A. Boyer, Michal MacArthur, Ricardo A. Cordova, Alexander W. Loftus, Christina S. Boutros, Jonathan J. Hue, Parnian Naji, Soubhi Tahhan, Peter Gallagher, William C. Beegan, Danyal Shah, James Choi, Nimat Manzoor, , Jordan Michael Winter

This study shows that a ketogenic diet enhances the effectiveness of glutamine metabolism inhibitors in pancreatic cancer by increasing tumor reliance on glutamine for energy.

Cell Reports Medicine · Apr 23, 2026Peer-reviewedCancer Biology

Cleavage of histone H2A during embryonic stem cell differentiation destabilizes nucleosomes to counteract gene activation

Mariel Coradin, Elizabeth G. Poulsen Porter, Francisca N. de Luna Vitorino, Richard M. Searfoss, Joseph Cesare, Yemin Lan, Zhexin Zhu, Peder J. Lund, Simone Sidoli, Yekaterina Perez, Congcong Lu, Justin Brumbaugh, Charles W.M. Roberts, Benjamin Aaron Garcia

The paper shows that cleavage of histone H2A by Cathepsin L during ESC differentiation reduces nucleosome stability and counteracts gene activation.

Journal of Biological Chemistry · Apr 10, 2026Peer-reviewedDevelopmental Biology

The conserved N-terminal SANT1-binding domain (SBD) of EZH2 Regulates PRC2 Activity

Agata L. Patriotis, Yadira M. Soto‐Feliciano, Douglas Barrows, Laiba F Khan, Marylène Leboeuf, Peder J. Lund, Matthew R. Marunde, Annaelle Djomo, Michael‐Christopher Keogh, Thomas S. Carroll, Benjamin A. Garcia, Alexey A. Soshnev, C. David Allis

This study investigates how the conserved SBD of EZH2 regulates PRC2 activity, showing its necessity for histone methylation and cancer cell proliferation.

Genes & Development · Feb 9, 2026Peer-reviewedGenetics

An optimized and robust workflow for quantifying the canonical histone ubiquitination marks H2AK119ub and H2BK120ub by LC-MS/MS

Mariana de Camargo Lopes, Peder J. Lund, Benjamin Aaron Garcia

The paper develops a robust LC-MS/MS workflow for quantifying H2AK119ub and H2BK120ub histone ubiquitination marks, improving upon existing methods for C-terminal modifications.

Journal of Proteome Research · Nov 18, 2024Peer-reviewedBiochemistry

Microbiota-dependent histone butyrylation in the mammalian intestine

Leah A. Gates, Bernardo Sgarbi Reis, Peder J. Lund, Matthew Robert Paul, Marylène Leboeuf, Zara Nadeem, Tom S. Carroll, Benjamin A. Garcia, Daniel Mucida, C. David Allis

The paper shows that histone butyrylation in intestinal epithelial cells is regulated by microbiota and the metabolite tributyrin, and is linked to gene expression.

bioRxiv · Sep 29, 2022 · PreprintMolecular Biology

Multi-omic profiling of histone variant H3.3 lysine 27 methylation reveals a distinct role from canonical H3 in stem cell differentiation

Yekaterina Kori, Peder J. Lund, Matteo Trovato, Simone Sidoli, Zuo‐Fei Yuan, Kyung‐Min Noh, Benjamin A. Garcia

The paper investigates the role of H3.3K27me3 in mouse embryonic stem cell differentiation, showing it regulates distinct terminal differentiation genes compared to canonical H3K27me3.

Molecular Omics · Jan 12, 2022Peer-reviewedGenomics

Quantitative Method for Assessing the Role of Lysine & Arginine Post-Translational Modifications in Nonalcoholic Steatohepatitis

Aaron Robinson, Aleksandra Binek, Vidya Venkatraman, Brian C. Searle, Ronald J. Holewinski, George Rosenberger, Sarah J. Parker, Nathan Basisty, Xueshu Xie, Peder J. Lund, Gautam Saxena, José María Mato, Benjamin Aaron Garcia, Birgit Schilling, Shelly Chi-Loo Lu, Jennifer E. Van Eyk

The paper introduces a method to quantify lysine and arginine PTMs in NASH using mass spectrometry and applies it to mouse liver lysates, finding altered methylation and acetylation in NASH.

Journal of Proteome Research · Sep 23, 2020Peer-reviewedSystems Biology

PROFILING OF Y1 CELLS TREATED WITH FGF-2 REVEALS PARALLELS WITH ONCOGENE-INDUCED SENESCENCE

Peder J. Lund, Mariana de Camargo Lopes, Simone Sidoli, Mariel Coradin, Francisca N. de Luna Vitorino, Júlia Pinheiro Chagas da Cunha, Benjamin Aaron Garcia

The paper profiles Y1 cells treated with FGF-2, revealing epigenetic and transcriptomic changes consistent with oncogene-induced senescence.

bioRxiv · Aug 17, 2020 · PreprintSystems Biology