Sara M. Nowinski

Engineered Tn5 Variants Enhance CUT&Tag Fidelity for Transcription Factors

This paper improves CUT&Tag for transcription factors by engineering Tn5 variants, enhancing recovery of TF binding sites in mouse cells and revealing distinct chromatin interaction modes.

Functional partitioning of lipoic acid decouples cellular abundance from mitochondrial utilization

This study shows that lipoic acid exists in two distinct cellular pools with different functions, and supplementation increases free LA without restoring mitochondrial function.

Cells Engage Endogenous Malonate Synthesis to Drive Mitochondrial Metabolism

The paper identifies malonate as an endogenous intermediate supporting mitochondrial fatty acid synthesis and oxidative phosphorylation, showing ACC1-dependent synthesis from glucose.

Carbohydrate adaptation drives liver-brain axis maturation

The paper shows that LACTB2 in the liver regulates mitochondrial maturation to coordinate carbohydrate metabolism with brain development in mice.

Mitochondrial Phosphopantetheinylation is Required for Oxidative Function

This study identifies AASDHPPT as a mitochondrial PPTase essential for mtFAS and oxidative function, with human variants linked to pathogenic effects.

Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4+ T Cell Function and Oxidative Metabolism

This study shows that Mecr, a mitochondrial fatty acid synthesis gene, is critical for CD4+ T cell metabolism, function, and survival, with implications for inflammatory diseases and mitochondrial disorders.

Cardiolipin deficiency disrupts electron transport chain and drives steatohepatitis

This study shows that mitochondrial cardiolipin deficiency disrupts electron transport chain function, leading to oxidative stress and promoting MASH in mice and humans.

Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency

The paper identifies biallelic MCAT variants in a patient with combined oxidative phosphorylation deficiency and shows reduced ETC complex subunits and enzyme activities.