Stefano Giustino Manzo

Transcription controls chromatin-nuclear lamina contacts through distinct Lamin A and LBR tethering mechanisms

This study shows that transcription inhibition rapidly and reversibly rewires chromatin-nuclear lamina contacts through distinct Lamin A and LBR tethering mechanisms.

Coordinated control of genome-nuclear lamina interactions by Topoisomerase 2B and Lamin B receptor

The paper identifies TOP2B as a regulator of genome-nuclear lamina interactions, showing its role in partitioning genomic regions between the nuclear lamina and interior, with LBR playing a complementary role.

Megabase-scale detachment of genome-nuclear lamina interactions is required for efficient repair of double stranded breaks

The paper shows that DSBs in LADs cause megabase-scale detachment from the nuclear lamina, which is necessary for efficient repair.

Acute multi-level response to defective de novo chromatin assembly in S-phase

This study investigates the acute effects of CAF-1 loss on chromatin assembly during S-phase, revealing multi-level responses including replication slowdown and histone modification changes.

Chromatin protein complexes involved in gene repression in lamina-associated domains

The paper identifies chromatin regulators involved in gene repression in lamina-associated domains (LADs) through genetic screens, showing that transcription elongation and chromatin remodeling factors, not nuclear lamina proteins, mediate repression.

Chromatin context-dependent effects of epigenetic drugs on CRISPR-Cas9 editing

This study identifies 67 epigenetic drugs that modulate CRISPR-Cas9 editing efficiency and repair outcomes based on chromatin context in human cells.

Perturbations in 3D genome organization can promote acquired drug resistance

The paper shows that disrupting nuclear lamina association promotes ABCB1 gene derepression and taxol resistance in RPE cells.

Optimisation of TP53 reporters by systematic dissection of synthetic TP53 response elements

The paper systematically examines how TP53 binding site architecture affects transcriptional output and develops a model to predict reporter activity.

Dynamic chromosomal interactions and control of heterochromatin positioning by Ki67

The paper investigates Ki67's role in chromatin organization by analyzing its genomic interactions and dynamics during the cell cycle in human cells.

CTCF and cohesin promote focal detachment of DNA from the nuclear lamina

The paper investigates the role of CTCF and cohesin in LAD positioning, finding they reinforce borders but do not position LADs, and mediate fine-tuning of nuclear lamina interactions.

Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance

The paper measures how chromatin context influences the balance between DNA repair pathways after Cas9-induced breaks in over 1,000 genomic locations.

DNA Topoisomerase I differentially modulates R-loops across the human genome

This study shows DNA Topoisomerase I regulates R-loop formation by causing gains and losses at different genomic regions, affecting replication and cell cycle progression.