René F. Ketting

This study shows that phosphorylation of Z granule components regulates germ granule organization and RNAi inheritance in C. elegans.

The paper identifies MUT 15 as a pseudohelicase in the CPUG complex that remodels the nuclease module to regulate RNA cleavage and poly(UG)ylation.

The paper shows that GTSF-1 binds RRF-3 in nematodes, a conserved interaction essential for 26G-RNA production and fertility.

The paper uses atomistic simulations to study contact dynamics in MUT-16 condensates, finding short-lived contacts and identifying key interactions and factors influencing condensate behavior.

The paper identifies an SLBP-independent mechanism for maintaining maternal histone mRNA stability in C. elegans oogenesis, mediated by the PETISCO complex and TOST-1.

The paper identifies a novel regulatory mechanism involving proteolytic processing of N-terminal intrinsically disordered regions in C. elegans Argonaute proteins, which controls small RNA selectivity and activity.

The paper investigates the developmental dynamics and crosstalk between WAGO-1 and WAGO-3 in C. elegans, showing distinct regulatory roles and complex interactions with 22G-RNAs and 26G-RNAs.

This study uses multiscale simulations to investigate MUT-16 phase separation and its role in recruiting MUT-8, confirming key interactions through in vitro experiments.

The paper shows that Meioc-Piwil1 complexes regulate rRNA transcription in zebrafish spermatogonial stem cells, preventing Piwil1 from localizing to nucleoli and promoting differentiation.

The paper identifies ERH and associated factors as post-transcriptional regulators of JAK2 mRNA maturation, essential for IFNγ signaling and innate immunity.

This study shows that maternal Tdrd6a and Tdrd6c are essential for germplasm stability, germ cell specification, and fertility in zebrafish.

This study identifies the roles of argonaute proteins in RNAi inheritance in C. elegans, showing HRDE-1 is required for silencing while WAGO-3 is essential for inheritance.

This study identifies two functions of the MUT7-C domain: RNA binding and recruitment to germ granules, revealing its ancestral role in MUT-7 exoribonuclease activity.

The paper identifies miR214 and dnm3os as regulators of sex determination in zebrafish, showing miR214 promotes female development through gsdf and dnm3os has a maternal effect on sex determination.

The paper identifies residues in Pasha's WW domain critical for Microprocessor assembly and localization in C. elegans.

The paper identifies PUCH, a trimeric Schlafen-domain nuclease that initiates piRNA processing in C. elegans by cleaving 5-end piRNA precursors, revealing a novel RNA endonuclease mechanism.

This study identifies a novel interaction between BmGtsf1L and BmVreteno's eTudor domain that facilitates piRNA biogenesis in Bombyx mori by connecting piRNA-loaded BmAgo3 and Gtsf1L.

The paper identifies LOTR-1, a C. elegans protein with LOTUS and Tudor domains, which interacts with ZNFX-1 to regulate small RNA processing in the germline.

The paper identifies maternally-deposited antisense piRNAs that silence transposons in zebrafish embryos by targeting young LTR retrotransposons and promoting H3K9me2/3 modification.

The paper shows that proteolytic processing of Argonaute proteins by DPF-3 regulates small RNA association, maintaining genome integrity and fertility in C. elegans.

The paper investigates post-migratory zebrafish primordial germ cells, finding delayed gene activation, nuclear gyration, and piRNA pathway activation without nuclear Piwi proteins.

The paper identifies PID-2 and related proteins as essential for establishing and maintaining RNAe in C. elegans embryos through interactions with eTudor domain proteins and proteolysis.

The paper investigates maternal and zygotic gene regulatory effects of endogenous RNAi pathways in C. elegans, revealing how sRNA-Argonaute complexes influence gene silencing across generations.

The paper shows that RppH can replace TAP for small RNA library preparation, preserving 5’-triphosphate groups and maintaining comparable results.

The paper identifies PETISCO as a novel protein complex essential for 21U RNA biogenesis and embryonic viability in C. elegans, revealing its distinct roles from PID-1.

This study identifies Tdrd6a as a regulator of Buc protein aggregation, which is essential for forming subcellular compartments that drive germ cell specification in zebrafish.

The paper identifies GTSF-1 as a protein required for the formation of an RNA-dependent RNA Polymerase complex in C. elegans, which is essential for 26G-RNA generation.