imputation

Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells

This paper introduces fluorogenic DNA-PAINT probes that improve super-resolution imaging speed and signal-to-background ratio in cells.

An Atlas of Indian Genetic Diversity

The paper presents a comprehensive genomic atlas of Indian populations, identifying novel variants and highlighting genetic diversity and pharmacogenomic implications.

In Vivo Metabolic Tagging and Targeting of Circulating Red Blood Cells

This paper develops an in vivo method to label and target red blood cells with azido groups, enabling long-term cargo delivery and improved imaging.

VEFill: a model for accurate and generalizable deep mutational scanning score imputation across protein domains

VEFill is a gradient boosting model that imputes missing DMS scores across protein domains, achieving strong predictive performance and generalization to new proteins.

Statistical BURST imaging for high-fidelity biomolecular ultrasound

This study improves BURST imaging by using statistical metrics to reduce background noise and enhance detection reliability in molecular imaging.

Kinetic design of reversible probe exchange enables continuous single-molecule tracking beyond the photobleaching limit

This paper introduces a method to enable continuous single-molecule tracking beyond photobleaching limits by designing reversible probe exchange kinetics.

Temporal Focusing for Enhanced Background Rejection in AOD-Based Two-Photon Serial Holography

This paper introduces a method to enhance background rejection in two-photon microscopy by integrating temporal focusing with holographic wavefront shaping.

Constructing a single-objective oblique plane microscope (OPM) for fast, multi-colour, high-resolution volumetric fluorescence imaging

This paper provides a protocol for building and characterizing an oblique plane microscope (OPM) to enable fast, multi-color, high-resolution volumetric fluorescence imaging with reduced phototoxicity.

Scanless temporal focusing enables high-speed three-dimensional quantitative phase microscopy

This paper introduces a scanless, high-speed 3D quantitative phase microscopy technique with improved resolution and reduced noise, enabling label-free volumetric imaging of biological samples.

A Modular In-Incubator Microscope for Longitudinal Live Cell Microscopy

This paper introduces an automated in-incubator microscope for long-term live cell imaging, enabling high-frequency, continuous data collection with improved thermal stability and operational reliability.

A foundation AI model enhances electron microscopy image analysis

DF5T improves EM image quality through five tasks, enhancing segmentation and 3D restoration of organelles.

Single Capture Quantitative Oblique Back-Illumination Microscopy

This paper introduces single-capture qOBM, a deep learning-enhanced technique for fast, label-free 3D phase imaging, achieving accuracy comparable to traditional methods with improved speed and simplicity.

SpaGene: A Deep Adversarial Framework for Spatial Gene Imputation

SpaGene improves spatial transcriptomics by imputing missing gene expressions, achieving higher accuracy than existing methods and revealing immune dynamics in lung tumors.

MetaGLIMPSE: Meta Imputation of Low Coverage Sequencing Data for Modern and Ancient Genomes

MetaGLIMPSE improves low coverage sequencing imputation by combining multiple reference panels, outperforming single-panel methods for modern and ancient DNA samples across various coverage and allele frequency ranges.

Characterizing MINFLUX imaging performance with DNA origami

The paper characterizes MINFLUX imaging performance over extended durations using DNA origami and corrects residual drift, achieving sub-2 nm precision without loss of site precision.

QuickStainer: a rapid negative staining device for improved preservation of molecular structure

QuickStainer improves preservation of labile macromolecules by rapidly staining samples with minimal incubation time on the carbon grid.

A rapid, low-cost approach to solid immersion lens fabrication for enhanced resolution in optical microscopy

This paper presents a low-cost, rapid method to fabricate solid immersion lenses (SILs) that enhance optical microscope resolution, achieving performance comparable to commercial glass SILs at a fraction of the cost.

MACHINE LEARNING FOR MISSING DATA IMPUTATION IN ALZHEIMER'S RESEARCH: PREDICTING MEDIAL TEMPORAL LOBE FLEXIBILITY

This paper uses machine learning to impute missing data in Alzheimer's research, finding that MissForest with Random Forest improves predictions of MTL flexibility.

Curved Axially Scanned Light-Sheet Microscopy

The paper introduces curved axially scanned light-sheet microscopy to overcome field curvature limitations in light-sheet imaging, increasing the usable field of view while maintaining sub-cellular resolution.

A reference panel for linkage disequilibrium and genotype imputation using whole-genome sequencing data from 2,680 participants across India

The paper constructs a large-scale LD and genotype imputation reference panel using whole-genome sequencing data from 2,680 Indian participants, improving imputation accuracy and polygenic risk score performance.

Reaction-enabled, highly sensitive super-resolution imaging

This paper introduces RIED, a super-resolution imaging method using electrochemiluminescence, achieving high sensitivity and resolution for intracellular structures.

PRISM: Niche-informed Deciphering of Incomplete Spatial Multi-Omics Data

PRISM improves spatial multi-omics analysis by imputing missing data and identifying spatial domains, outperforming existing methods on simulated and real datasets.

Robust quality assessment of cryo-EM maps, tomograms and micrographs by statistics-based local resolution estimation

The paper introduces a statistics-based method for local resolution estimation in cryo-EM maps, tomograms, and micrographs to improve quality assessment and reconstruction.

spRefine Denoises and Imputes Spatial Transcriptomics with a Reference-Free Framework Powered by Genomic Language Model

spRefine denoises and imputes spatial transcriptomics data using a reference-free deep learning framework, improving data integration and revealing new aging-related biological insights.

Microbubble Backscattering Intensity Improves the Sensitivity of Three-dimensional (3D) Functional Ultrasound Localization Microscopy (fULM)

This paper introduces backscattering fULM (B-fULM) to improve 3D functional ultrasound imaging sensitivity by integrating microbubble amplitude with count-based localization.

Caging-group-free photoactivatable fluorophores with far-red emission

This paper introduces caging-group-free photoactivatable fluorophores with far-red emission for improved super-resolution microscopy.

ARUNA: Slice-based self-supervised imputation for upscaling DNA methylation sequencing assays

Aruna upscales sparse DNA methylation data from RRBS to whole-genome resolution using a self-supervised denoising autoencoder, outperforming existing methods in simulations and real data.

Optical Coherence Tomography with Fluorescein Optical Clearing for Transscleral Image Guidance

This study uses fluorescein as an optical clearing agent to enhance OCT imaging depth in scleral tissues, improving transscleral image guidance during surgery.

High Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM

This paper introduces SP-STORM, a method combining single molecule photoswitching and spectral phasor analysis to achieve high-throughput, multiplexed super-resolution imaging of subcellular structures.

Membrane-Promoted J-Aggregation of BODIPY Dimers Enables Spontaneous Blinking in Green and Red Channels for Live-Cell Nanoscopy

This paper designs BODIPY dimers that form J-aggregates in cell membranes, enabling brighter and more precise live-cell single-molecule localization microscopy in green and red channels.