imputation

Broadband backscattering confocal microscopy enables label-free 3D live cell nanoscale sensitive imaging

This paper introduces broadband backscattering confocal microscopy (BBCM) for label-free 3D live cell imaging with improved contrast and size sensitivity.

Streamlining large-scale high-resolution electron tomography with VolWeaver

The paper optimizes a transmission electron tomography workflow for malaria parasites and introduces VolWeaver to integrate high-resolution tomographic data into serial section volume reconstructions.

Space-Time Light-Sheet Microscopy

This paper introduces space-time light-sheet microscopy (ST-LSM), a single-objective technique that improves imaging volume and field of view while maintaining resolution.

Enhanced 2D structured illumination microscopy: super-resolution with optical sectioning and reduced reconstruction artifacts

This paper introduces enhanced 2D-SIM to reduce reconstruction artifacts and improve resolution in thick samples using optimized coarse and fine patterns.

Broadband electronic resonance coherent anti-Stokes/Stokes Raman scattering microscopy

This paper introduces BER-CARS/CSRS microscopy, which enables highly sensitive, label-free imaging of endogenous chromophores with broad spectral coverage, applied to living cells and tissues.

Robust thermometry-imaging at sub-micrometer and millisecond-resolution by fluorescence lifetime microscopy allows for additional acquisition of multiple imaging channels

The paper introduces a method to measure temperature with high precision and resolution using fluorescence lifetime microscopy with Cy5 dye, enabling simultaneous multi-channel imaging.

Coherent Structured Illumination Microscopy with Enhanced Optical Sectioning

This paper introduces a 5-beam c-SIM technique that improves optical sectioning and lateral resolution in non-fluorescent samples by suppressing coherent defocus effects.

Hybrid Solid-Liquid Optics Enable Scalable, High-Resolution, Multi-Immersion Light-Sheet Microscopy

This paper introduces a new optical framework and imaging devices that enable scalable, high-resolution, multi-immersion light-sheet microscopy for biological samples.

Evaluation of fluorescent proteins for compatibility with STED microscopy systems using two-color spectroscopies

The paper evaluates fluorescent proteins for STED microscopy compatibility using two-color spectroscopy, showing improved 3D resolution in compatible proteins.

Temporal Focusing for Enhanced Background Rejection in AOD-Based Two-Photon Serial Holography

This paper introduces a method to enhance background rejection in two-photon microscopy by integrating temporal focusing with holographic wavefront shaping.

Reaction-enabled, highly sensitive super-resolution imaging

This paper introduces RIED, a super-resolution imaging method using electrochemiluminescence, achieving high sensitivity and resolution for intracellular structures.

Large field of view fluorescence imaging of microfluidic devices with a tandem-lens macroscope

This paper introduces a macroscope for wide-field fluorescence imaging in microfluidic devices, achieving high sensitivity and temporal resolution over a large area.

Spatial Polarization-Induced Fluorescence Fluctuation Imaging (SPIFFI) Enables Single-shot Super-Resolution and Multidimensional Imaging

SPIFFI enables single-shot super-resolution and six-dimensional imaging by leveraging polarization-induced fluorescence fluctuations, enhancing live-cell imaging capabilities.

Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells

This paper introduces a single-objective lattice light sheet microscopy platform for improved 3D single-molecule super-resolution imaging in scattering samples.

All-Optical Strategies to Minimize Photo-Bleaching in Reversibly Switchable Fluorescent Proteins

The paper develops all-optical strategies to reduce photo-bleaching in reversibly switchable fluorescent proteins, enhancing imaging resolution and duration.

Azimuthal Segment Imaging in cryo-STEM Tomography

This paper introduces parallax-filtered cryo-STET imaging to enhance contrast in thick biological specimens, demonstrating improvements over traditional STEM methods.

Open Raman Microscopy (ORM): A Modular Hardware and Software Framework for Accessible Raman Imaging

The paper introduces an open-source Raman microscopy framework with modular hardware and software for accessible chemical imaging across various applications.

Parallel frequency-multiplexed aberration measurement for widefield fluorescence microscopy

This paper introduces PFAM and PFAM-SIFT methods for parallel aberration measurement in widefield fluorescence microscopy, enabling accurate correction of spatially varying aberrations in complex samples.

Single-aperture SLAM microscopy with amplitude-tailored vector beams

This paper introduces a single-aperture SLAM microscope using vector beams to achieve sub-diffraction-limited resolution in living tissue.

Two-Dimensional Nonlinear Structured Illumination Microscopy with rsEGFP2

The paper demonstrates 2D nonlinear structured illumination microscopy (NSIM) using rsEGFP2 to achieve sub-80 nm resolution for live-cell actin imaging.

SIMple: A fibre-based platform for accessible structured illumination microscopy

The paper introduces a fibre-based structured illumination microscopy (SIM) system that simplifies setup and improves resolution without specialized optics.

Magellon - an extensible platform for cryo-EM data visualization, management, and processing

Magellon provides a flexible and scalable cryo-EM platform with microservices architecture for data management, processing, and visualization.

Cleared tissue dual-view oblique plane microscopy

This paper introduces a dual-view oblique plane microscope for high-resolution imaging of thick cleared tissue samples across multiple species.

Super-resolved live imaging of thick biological samples with 3D Random Illumination Microscopy (3D-RIM)

This paper introduces 3D-RIM, a method that achieves super-resolved volume imaging of thick live specimens with high contrast and resolution across multiple microns.

Pan-ASLM: a high-resolution and large-field-of-view light sheet microscope for Expansion Microscopy

The paper introduces pan-ASLM, a light sheet microscope optimized for expansion microscopy, achieving higher speed, larger field of view, and better resolution than standard confocal microscopes.

Superresolution imaging of live samples by centroid reassignment microscopy

This paper introduces a superresolution microscopy technique called centroid reassignment microscopy (CRM) that enables fast, robust, and protocol-compatible imaging of live samples.

Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment

This paper introduces a DNA-PAINT system with optical photon reassignment on a spinning disk confocal to achieve high-resolution imaging in thick samples and large fields of view.

A Spectral Image Scanning Microscope for Multi-Color High-Resolution Imaging

This paper introduces a spectral CSD-ISM system for high-resolution, multi-color imaging with improved speed and resolution.

DNA-PAINT imaging with hydrogel imprinting and clearing

This study shows that polyacrylamide hydrogels support DNA-PAINT imaging by preserving nanoscale docking strand positioning and enhancing diffusion and accessibility.

Mesoscopic SCAPE Microscope with a Rescanned, Super-oblique Illumination Plane

This paper introduces a mesoscopic SCAPE microscope with a super-oblique light sheet and rescanning strategy, achieving high-resolution volumetric imaging over large fields of view.