Bacterial biofilms and quorum sensing

Stress-hardening behaviour of biofilm streamers

This study shows biofilm streamers exhibit stress-hardening behavior, with mechanical properties increasing with external stress, driven by extracellular DNA and RNA.

A Chemostat-Based Model for Growing Bacterial Biofilms

This paper introduces a chemostat-based experimental platform to study bacterial biofilms and their interactions with environments, showing biofilms grow larger and produce migrants in chemostats.

Modulation of the Pseudomonas aeruginosa quorum sensing cascade by MexT-regulated factors

This paper identifies MexT-regulated factors that modulate quorum sensing hierarchy in Pseudomonas aeruginosa, revealing alternative QS architectures in clinical isolates.

An effective response to respiratory inhibition by a Pseudomonas aeruginosa excreted quinoline promotes Staphylococcus aureus fitness and survival in co-culture

This study shows that Pseudomonas aeruginosa's HQNO inhibits Staphylococcus aureus respiration, activating SrrAB to enhance fermentation and survival in co-culture.

Inhibition of agr Quorum Sensing in Staphylococci by Novel Thiazole-Indole Hybrid Compounds

This study identifies two thiazole-indole hybrid compounds that inhibit Staphylococcus aureus quorum sensing without affecting bacterial viability, reducing virulence factors and biofilm formation.

AFM characterization of early P. aeruginosa aggregates highlights emergent mechanical properties

This study uses AFM to analyze the mechanical properties of early P. aeruginosa aggregates in CF sputum medium, showing increased deformation resistance compared to planktonic cultures.

Using a Bacterial Protein to Selectively Target Bacterial Biofilms: Treatment of S. epidermidis Biofilms with Targeted Photothermal Gold Nanoparticles

This paper develops targeted photothermal gold nanoparticles using a bacterial protein to selectively destroy S. epidermidis biofilms with high efficiency.

Chemical dysbiosis byproducts trigger predation via alternative activation of a peptide quorum sensor in salivarius streptococci

The paper shows that S. salivarius uses both peptides and organic molecules to activate its quorum sensor, revealing a new signaling mechanism in bacterial communication.

EPAS1 regulates host antibacterial defense in response to bacterial quorum sensing

This study shows EPAS1 acts as an intracellular receptor for bacterial quorum sensing signals, enhancing antibacterial defense by promoting cytokine production and improving survival in infected mice.

Intracellular glutamine fluctuates with nitrogen availability and regulates Mycobacterium smegmatis biofilm formation

This paper shows that intracellular glutamine levels, influenced by nitrogen availability, regulate biofilm formation in Mycobacterium smegmatis by affecting aggregation.

Real-time high-resolution microscopy reveals how single-cell lysis shapes biofilm matrix morphogenesis

The paper uses real-time microscopy to show that single-cell lysis in Pseudomonas aeruginosa biofilms is spatially restricted and driven by nutrient gradients, shaping eDNA matrix morphogenesis.

Pseudomonas aeruginosa lasR is a keystone gene in polymicrobial cultures

This study shows that loss of lasR function in Pseudomonas aeruginosa disrupts inter-species interactions in polymicrobial CF airway infections, leading to instability in Candida albicans titres and transcriptional reprogramming.

Mucosal vaccine immunity induced by a new auxotrophic Pseudomonas aeruginosa strain is linked to Th17 and IgA responses

This study shows a new auxotrophic Pseudomonas aeruginosa vaccine strain induces Th17 and IgA responses, offering mucosal protection against infection.

Host- and microbial-mediated mucin degradation differentially shape Pseudomonas aeruginosa physiology and gene expression

This study shows that microbial degradation of mucins provides more nutrients and triggers gene expression changes in Pseudomonas aeruginosa than host-mediated mucin breakdown.

Pseudomonas aeruginosa uses kinases NahK and RetS to control the motile-biofilm switch

This study identifies NahK and RetS as key regulators of the Gac-MKN in Pseudomonas aeruginosa, showing their roles in controlling RsmA activity and the motile-biofilm switch.

Structural Basis of Pseudomonas FapC Biofilm-Forming Functional Amyloid Formation

This study determines the structural basis of FapC biofilm formation in Pseudomonas aeruginosa using integrative structural biology methods.

Mapping genetic and phenotypic diversity of Pseudomonas aeruginosa across clinical and environmental isolation sites

The paper analyzes 125 Pseudomonas aeruginosa isolates from clinical and environmental sources to assess genotype-phenotype correlations and niche specialization, finding that genomic diversity does not correlate with isolation source or most virulence traits, but Group B strains show higher cytotoxicity.

Metabolic Strategies That Enable Oral Commensal Persistence in a Lower Airway Environment

This study identifies metabolic strategies enabling Neisseria mucosa persistence in the inflamed lower airway, highlighting nitrate respiration and antibiotic resistance as key factors.

Pseudomonas aeruginosa biofilm-deficient mutants undergo parallel adaptation during chronic infection

This study identifies genetic adaptations in Pseudomonas aeruginosa during chronic infection that lead to hyperbiofilm phenotypes through mutations in lipopolysaccharide and type IV pili biosynthesis.

Inactivation of MexT in Pseudomonas aeruginosa destabilizes cooperation and favors the emergence of a unique quorum sensing variant

The paper shows that inactivating MexT in Pseudomonas aeruginosa destabilizes cooperation and promotes the emergence of a specific LasR variant.

ComFB, a new widespread family of c-di-NMP receptor proteins

The paper identifies ComFB as a new family of c-di-NMP receptors in bacteria, showing they bind c-di-GMP and c-di-AMP and regulate cellular processes like motility.

Pseudomonas aeruginosa LasR-deficient mutants have increased methylglyoxal and hydrogen peroxide sensitivity due to low intracellular glutathione

This study shows that Pseudomonas aeruginosa LasR mutants are more sensitive to methylglyoxal and hydrogen peroxide due to reduced intracellular glutathione.

Nitric oxide donor sodium nitroprusside serves as a source of iron supporting Pseudomonas aeruginosa growth and biofilm formation

This study shows that sodium nitroprusside (SNP) promotes Pseudomonas aeruginosa biofilm and planktonic growth by acting as an iron source, not just as an NO donor, which may confound biofilm dispersal studies.

wspA mutation mediated Pseudomonas aeruginosa rugose small colony variant and its hyper-biofilm

This study identifies a wspA mutation in Pseudomonas aeruginosa that increases c-di-GMP synthesis, leading to rugose small colony variant formation and enhanced biofilm-mediated antibiotic resistance.

Biofilm Formation by Pseudomonas aeruginosa in Medium Supplemented with Adult Bovine Serum and the Anti-Biofilm Activity of β-Lactam Antibiotics

This study evaluates the anti-biofilm activity of beta-lactam antibiotics against Pseudomonas aeruginosa biofilms formed in medium with adult bovine serum.

Pseudomonas aeruginosa supports the survival of Prevotella melaninogenica in a cystic fibrosis lung polymicrobial community through metabolic cross-feeding

This study identifies a cross-feeding mechanism between Pseudomonas aeruginosa and Prevotella melaninogenica in a CF lung polymicrobial community, where metabolites from mucin degradation support P. melaninogenica survival.

SadB acts as a master regulator modulating Pseudomonas aeruginosa pathogenicity

This study shows that SadB in Pseudomonas aeruginosa regulates virulence, biofilm formation, and quorum sensing, with its deletion leading to attenuated virulence and biofilm defects.

Calprotectin protects Staphylococcus aureus in coculture with Pseudomonas aeruginosa by attenuating quorum sensing and decreasing the production of pseudomonal antimicrobials

This study shows calprotectin protects Staphylococcus aureus in coculture with Pseudomonas aeruginosa by reducing quorum sensing and antimicrobial production in P. aeruginosa, while enhancing S. aureus' survival.

Native-State Imaging Reveals Spatially Separated Organized Cells and Strain-Specific Matrix Architecture in Pseudomonas aeruginosa Biofilms

This study uses advanced imaging techniques to reveal the spatial organization of Pseudomonas aeruginosa biofilms, showing sparse cell distribution and strain-specific matrix structures.

Pseudomonas aeruginosa phenazines dictate site-specific competitive interactions with Klebsiella pneumoniae

This study shows that Pseudomonas aeruginosa uses phenazines to inhibit Klebsiella pneumoniae growth, with strain-specific and site-dependent effects.