Glycosylation and Glycoproteins Research

The paper determines the cryoEM structures of MUC5AC variants and shows their structural basis for mucus formation and disease risk in COPD and IPF.

The paper identifies glycan structures on flagellin proteins of Aeromonas hydrophila, revealing metaheterogeneity and a novel pseudaminic acid derivative.

This paper characterizes the glycosylation of soluble CD52 and identifies its role in immune suppression through interactions with HMGB1 and SIGLEC-10.

This study identifies miRNAs that regulate CMAS expression, impacting sialylation levels and showing bidirectional miRNA regulation in pancreatic cancer.

The paper compares glycosylation patterns of IgA1 and IgA2, finding subclass-specific differences despite similar glycosylation enzyme expression.

This study identifies N-acetyl-β-D-hexosaminidase as a driver of colorectal cancer progression and a prognostic marker in patient plasma.

This paper develops specific antibodies to detect O-fucose on nucleocytoplasmic proteins in protists, slime molds, and plants, revealing conservation of the O-fucome across species.

This study investigates how MUC2 expression affects immune infiltration in colorectal cancer using in vitro models and CRISPR knockout, finding that MUC2 loss increases immune infiltration only in HT-29 cells.

This study identifies the unknown moiety in the b-type flagellin glycan of Pseudomonas aeruginosa PAO1 as N,N-dimethylthreonine using mass spectrometry and compares it to Type A glycan in C. difficile.

This paper develops a mass spectrometry-based method to decode glycoproteins by integrating deglycosylation and glycosylation site characterization, enabling accurate sequencing of complex glycosylated proteins.

The paper shows that cell membrane glycan content creates a kinetic barrier to viral uptake in a protein-nonspecific way by forming a physical obstacle to viral adhesion.

This study develops a nanobody-enzyme fusion protein targeting PD-L1 and sialic acid, showing anti-tumor effects by modulating tumor-associated macrophages.

This study characterizes the N-glycan structures and their impact on the structural dynamics and functional properties of the Abdala COVID-19 vaccine.

The paper uses lectin microarrays to analyze glycoproteins in salivary glands of Aedes aegypti and Anopheles stephensi, finding similar glycan profiles with specific oligomannose and O-glycan presence.

This study shows that reducing OGT activity in mice causes embryonic lethality proportional to the reduction in O-GlcNAc modification, with milder reductions causing developmental delays and retrovirus activation.

This study synthesizes sialylated and fucosylated mucin analogs to investigate glycan effects on protein conformation and degradation.

The paper identifies O-GlcNAcylation of DDX3X as a regulator of the G1/S-phase transition and shows its role in cell cycle control and neurogenesis in OGT-CDG.

The paper identifies and characterizes a fucose-binding lectin from Enterobacter cloacae with high specificity for LewisA and blood group O antigens.

The paper uses ZenoTOF mass spectrometry to analyze glycoproteomes from breast cancer and control samples, identifying 15 site-specific glycans as potential biomarkers for breast cancer detection.

This study compares N-glycosylation profiles of iPSCs and NSCs from DS and non-DS individuals, finding altered glycan structures in T21 cells during neural differentiation.

This paper introduces a framework for automated glycan nomenclature conversion that accepts any input format and outputs a standardized IUPAC-condensed format, enabling seamless integration of glycan data.

This study reveals the glycocalyx as an ordered, self-assembled hierarchical micro- and nanoarray structure conserved across evolution in human and sponge cells.

The paper identifies and characterizes O-link glycosylation in IgG4 antibodies using LC-MS and mass spectrometry analysis of Dupixent.

This study shows that SPPL3 in tumor cells affects innate immune responses by modulating nsGSLs, reducing susceptibility to neutrophils, NK cells, and γδ T cells.

This paper develops a chemoenzymatic method to precisely glyco-tag proteins, enabling the modification of therapeutic proteins with diverse glycans while maintaining their biological activity.

The paper develops OST inhibitors that selectively target STT3A and STT3B, leading to tumor regression in EGFR-mutant lung cancer models.

The paper identifies and validates substrate specificity of bacterial monotopic phosphoglycosyl transferases using bioinformatics and in vitro assays, supporting inhibitor discovery for antibiotic development.

This study uses metabolomics and 13C tracing to examine glucose allocation to membrane glycans in pancreatic cancer cell lines, revealing differences in glycan synthesis and metabolic pathways.

This paper develops multifunctional reagents for nanoscale imaging of glycans using expansion microscopy, validated in C. elegans and mammalian cells.

The paper develops linear and neural network models to predict N-glycosylation site glycan distribution in Chinese Hamster Ovary cells using B4GALT levels, achieving a 1.39% median prediction error.