Zebrafish Biomedical Research Applications

FishNET is an open-source relational database for managing zebrafish colony data, offering automated data entry, flexible queries, and integration of biological samples and records.

This study identifies interferon-stimulated gene and cellular stress pathway activation in zebrafish morpholino-injected embryos, suggesting morpholinos may trigger innate immune responses.

The paper identifies 68 compounds that reduce versican b expression in zebrafish adgrg6 mutants and 41 that restore myelin basic protein gene expression, with 19 candidates potentially interacting directly with Adgrg6.

This study shows that DYRK1A regulates calcium signaling in zebrafish vascular development, and modulating calcium signaling can rescue vascular defects in DYRK1A knockouts.

Stytra is an open-source software for larval zebrafish behavioral experiments, enabling stimulus control, tracking, and data logging with modular design and extensibility.

The paper demonstrates the successful generation of stable transgenic lines in Astyanax mexicanus using the Tol2 transposase system, enabling the expression of fluorescent markers in specific cell types.

This study shows that ahr2, not ahr1a or ahr1b, is essential for craniofacial and fin development and mediates TCDD toxicity in zebrafish.

The paper improves in vivo imaging in fish by optimizing anaesthesia, fluorescent protein selection, and pigmentation removal.

This study evaluates the embryotoxicity and teratogenic effects of Curcuma longa extract on zebrafish embryos and larvae, finding dose-dependent toxicity and physical deformities at higher concentrations.

The paper presents a multi-sample imaging, processing, and analysis platform for studying vascular growth in zebrafish over time.

The paper examines how new spinal pathways from hindbrain V2a neurons develop in larval zebrafish to support the emergence of diverse locomotor behaviors postnatally.

This study investigates the role of Ntrk2b in maintaining dopamine and serotonin neurons in zebrafish, showing its specific impact on these aminergic systems.

This study shows human macrophages survive and adopt activated genotypes in zebrafish, mimicking cancer-related immune responses.

This paper introduces zGrad, a nanobody-based degron system for inducible and reversible protein inactivation in zebrafish, demonstrating its utility in studying protein function across different spatial and temporal contexts.

The paper generates and annotates new Cre transgenic zebrafish lines for intersectional genetic experiments and integrates them with a brain atlas for spatial search.

This study develops a high-throughput operant learning system for larval zebrafish and shows that lower visual intensity ratios enhance learning responses and memory extinction.

This paper develops a deep learning system for automated microinjection in zebrafish embryos, achieving efficiency comparable to manual methods and enabling high-throughput genome editing.

The paper identifies Npas4l target genes involved in cardiovascular development using zebrafish models and genome-wide approaches.

The paper shows that macrophage-mediated dynamic control of Il-1β and Tnf-α is essential for spinal cord regeneration in zebrafish.

The paper identifies biological motion as an innate perceptual cue driving shoaling in zebrafish using a virtual reality assay.

This study xenografts murine bone marrow cells into zebrafish embryos to study hematopoiesis and host-pathogen interactions, showing successful integration and immune response.

This study shows that ROS-induced repositioning of Hedgehog-expressing notochord cells drives zebrafish tail regeneration, highlighting a regeneration-specific role for developmental signaling.

This study compares aggressive display differences between leopard and longfin zebrafish and shows varying effects of acute fluoxetine on aggression in these phenotypes.

Zebrabase provides a scalable database for tracking aquatic model organisms, offering management, reporting, and visualization tools for research facilities.

The paper maps the connectome of zebrafish neuromasts, revealing specific, redundant, and hierarchical wiring patterns, and identifies Notch signaling as mediating wiring specificity.

The paper shows that sgIIb is critical for hindbrain neurovascular modeling in zebrafish through MAPK and PI3K/AKT signaling.

The paper develops a double binary zebrafish model to study myeloid gene regulation during melanocyte transformation and melanoma initiation.

The paper describes an optimized pipeline for genotyping zebrafish embryos after in situ hybridization and digitally quantifying gene expression, improving accuracy and reducing bias.

The paper develops a dynamic circuit model to explain how heat sensation is transformed into swimming behavior in larval zebrafish.

This study develops a technique to image whole brain neural activity in freely behaving larval zebrafish during specific behaviors.