Studies on Chitinases and Chitosanases

Dual Carbohydrate Recognition by the Chitinase-like Protein CHI3L1 Through Distinct Glycosaminoglycan and Chitin-Binding Interfaces

This study identifies two distinct carbohydrate-binding sites on CHI3L1, showing that chitin oligosaccharides and glycosaminoglycans bind to separate sites with different affinities and regulatory effects.

A fatty acid-binding protein links lipid handling to chitin synthase-dependent cuticle formation and nanoscale organization

This study identifies TcFabp8 as a fatty acid-binding protein essential for chitin synthase-dependent cuticle formation and nanoscale organization in Tribolium castaneum.

Carbohydrate-active enzymes of giant viruses: Molecular and biochemical characterization of glycosyl hydrolases from algae-infecting chloroviruses

This study characterizes glycosyl hydrolase 18 chitinases from chloroviruses, revealing their enzymatic properties, structural features, and potential as biotechnological tools for algal control.

Synthetic iminosugar monomers change global metabolic pathways and chitin biosynthesis in Thalassiosira rotula

The paper shows that iminosugar analogues alter chitin biosynthesis and metabolic pathways in Thalassiosira rotula by repressing carbohydrate pathways and inducing ribosome biogenesis.

Chitinase-3-like protein 1 decodes chitosan acetylation patterns into toll-like receptor 2 signaling through heparan sulfate

This study shows CHI3L1 interacts with chitosan and heparan sulfate to activate TLR2 signaling, revealing HS as a co-receptor for CHI3L1 and its role in inflammation.

Structural and chemical properties of insects' chitin-containing extracellular matrices

The paper investigates the structural and chemical properties of chitin in insect extracellular matrices, finding that PM chitin is highly acetylated and organized as dihydrate β-chitin, while cuticle chitin is deacetylated and structured as α-chitin.

A Bait-and-Switch strategy links phenotypes to genes coding for Polymer-Degrading Enzymes in Intact Microbiomes

The paper introduces a 'bait-and-switch' strategy to link enzyme function with community-level phenotypes in microbiomes, demonstrating its feasibility in identifying chitin-degrading enzymes.

Marine Aspergillus terreus produces a chitinase exhibiting a dual mode of enzymatic action

This study characterizes a chitinase from marine Aspergillus terreus that exhibits both endo- and exochitinase activities, producing GlcNAc and oligomers from different chitin substrates.

The modular evolution of chitinases is governed by coevolution of auxiliary and catalytic domains

This study maps the sequence space of bacterial chitinases to show how domain composition and catalytic core evolve differently based on functional roles.

Functional characterization of multi-domain LPMOs from marine Vibrio species reveals modulation of enzyme activity by domain-domain interactions

The paper functionally characterizes multi-domain LPMOs from marine Vibrio species, revealing that domain-domain interactions modulate enzyme activity by regulating copper site accessibility.

MoChia1 is a family 18 glycoside hydrolase from the rice blast fungus, Magnaporthe oryzae, that releases reducing-end GlcNAc from chitin oligosaccharides

This paper identifies MoChia1, a GH18 chitinase from Magnaporthe oryzae, which specifically hydrolyzes chitin oligosaccharides at the reducing end and is inhibited by allosamidin.

A dual-domain chitinase mechanism enables marine bacteria tosense and localize sparse crystalline chitin in the ocean

This study identifies a dual-domain chitinase in marine bacteria that enhances chitin binding and retention, improving resource acquisition in the ocean.

Generation of a Ym1 Deficient Mouse utilising CRISPR-Cas9 in CB6 Embryos

The paper generates a Ym1 deficient mouse using CRISPR-Cas9 in CB6 embryos and validates the absence of Ym1 protein without affecting related chitinase genes.

Mechanism of catalytic apparatus of human chitotriosidase-1 and its dual inactivation mode by the first-in-class OATD-01 inhibitor.

This study models the catalytic mechanism of human chitotriosidase-1 using structural data and simulations, and shows how the OATD-01 inhibitor disrupts enzyme function through dynamic changes.

Unveiling Novel Biocontrol Strategies: Serratia marcescens chiA Gene Against Myzus persicae

This study expresses and characterizes the chiA chitinase gene from Serratia marcescens GBS19, showing it has insecticidal activity against Myzus persicae with high similarity to reference sequences and strong chitin binding.

Functional insights into a peculiar tetra-modular LPMO from the human pathogen Enterobacter cloacae

This study functionally characterizes a tetra-modular LPMO from Enterobacter cloacae, revealing its chitin-cleaving activity and structural features involving copper coordination.

Structural and dynamic study of fungal cell wall degrading fungal chitinase and its interaction with chitooligosaccharide

This study uses molecular modeling and simulation to analyze the structure and dynamics of a fungal chitinase and its interaction with chitotriose, revealing key residues and stability mechanisms.

Kasugamycin inhibits melanoma lung metastasis and regulates CHI3L1-driven M2-like tumor-associated macrophage differentiation

Understanding bottlenecks in the microbial production of partially acetylated chitooligosaccharides

This study identifies bottlenecks in microbial production of partially acetylated chitooligosaccharides by analyzing co-expression of chitin deacetylases and a chitooligosaccharide synthase in E. coli.

Polysaccharide quantification using microbial enzyme cocktails

This study introduces a method using microbial enzyme cocktails to quantify polysaccharides, demonstrating effectiveness for chitin and laminarin with low detection limits.

Different cellular and molecular mechanisms of chitin deposition contribute to the specificity of the two chitin synthases in D. melanogaster.

This study investigates the distinct cellular and molecular mechanisms of two chitin synthases in D. melanogaster, showing they use different strategies to deposit chitin with specific functions.

Gene family expansion and functional diversification of chitinase and chitin synthase genes in Atlantic salmon (Salmo salar)

The paper investigates the expansion and functional diversification of chitinase and chitin synthase genes in Atlantic salmon using comparative genomics, transcriptomics, and chromatin accessibility data.

Dissecting the roles of Expansion/Rebuf and the chitin synthase Krotzkopf Verkehrt in chitin deposition in Drosophila

The paper investigates the roles of Expansion/Rebuf and Krotzkopf Verkehrt in chitin deposition in Drosophila, showing their distinct functions in polymerization and translocation.

Imaginal disc growth factors are Drosophila Chitinase-like Proteins with roles in morphogenesis and CO2 response

This study identifies Drosophila CLPs as Imaginal disc growth factors with roles in fertility, viability, epithelial morphogenesis, and CO2 response.

Characterization of chitin deacetylase genes in the Diaphorina citri genome

The paper characterizes four chitin deacetylase genes in the Diaphorina citri genome, providing a foundation for future studies on their potential roles in exoskeleton formation and bacterial defense.

Short distance non-autonomy and intercellular transfer of chitin synthase in Drosophila.

This paper investigates the role of chitin synthase in Drosophila cuticle resilience and shows short-range non-autonomy and potential intercellular transfer mechanisms.

The localization of chitin synthase mediates the patterned deposition of chitin in developing Drosophila bristles.

The paper shows that chitin synthase localization in Drosophila bristles mediates patterned chitin deposition through interactions with Rab11 and the actin cytoskeleton.