Discover, rate, and discuss biology preprints.

The paper presents CryoEM structures of Rad51 and Dmc1 in ADP-bound states and compares them to ATP-bound filaments, revealing structural transitions linked to ATP hydrolysis and filament disassembly.

The paper investigates the ester bond crosslinking mechanism in bacterial adhesin proteins, confirming a protease-like mechanism through structural, functional, and computational analyses.

This study clones and characterizes a novel acidic laminarinase from a hot spring metagenome, showing high activity at acidic pH and high temperature with potential industrial applications.

The paper identifies U-rich elements in 3' UTRs as drivers of cryptic splicing in MPRA, showing they affect splice site choice and expression levels.

This paper introduces EMERALD-ID, a method for identifying small molecules in cryo-EM data, achieving 43% exact identification and 66% close identification of common ligands.

This study shows polyserine domains self-assemble and interact with tau to form sites of aggregation, accelerating tau fibrilization in vitro.

This study identifies two distinct mechanisms for generating C- and G-circles in ALT cancers, involving different origins and initiation processes for each type of extrachromosomal telomere DNA.

This study shows that cysteine residues are critical for S100A6 binding to RAGE and reveals a covalent dimerization mechanism in S100 proteins.

This study identifies redox-dependent binding configurations of NAD(P)H in live cells using FLIM and modeling, revealing how redox states influence fluorescence decay.

This paper introduces DMS-Fold, a method that uses deep mutational scanning data to improve AlphaFold2 predictions, achieving better results for most protein targets.

This study uses solid-state NMR to examine how MW8 antibodies bind to the fuzzy coat of HTT exon 1 fibrils, revealing structural and dynamic effects on the fibrils.

This paper computationally designs and experimentally validates a single-domain antibody that specifically recognizes a WT1 peptide-loaded class I MHC complex with high affinity and specificity.

The paper shows MTCH1 can function as an insertase in mitochondria, compensating for the loss of the yeast MIM complex and restoring mitochondrial biogenesis and morphology.

This study identifies the molecular mechanism by which VcParE2 inhibits Gyrase and shows that VcParD2 neutralizes this activity by shielding Trp25.

This paper develops a GC-MS-based method to measure PEPC activity in vivo by analyzing positional 13C enrichment in aspartate from Synechocystis cultures.

This paper optimizes Bio-Layer Interferometry (BLI) protocols to measure binding affinities of antiviral compounds with viral proteins, demonstrating sub-nanomolar KD values and covalent interactions.

This study reveals the structural mechanism of cholesterol sensing by LYCHOS, showing how cholesterol binding modulates its interaction with GATOR1 to activate mTORC1.

The paper investigates how the metabolic system of Papilio memnon larvae transforms host plant components into bioactive substances, identifying two chlorophyll catabolites with anti-cancer and anti-amyloid properties.

This study identifies a conserved allosteric network in apoptotic caspases across chordates, revealing how conformational dynamics and residue interactions regulate their function over 500 million years of evolution.

This study investigates the activity-stability trade-off in PETase using simulations and experiments, revealing how reshaping loop dynamics can enhance both stability and activity.