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Transient matrix remodeling during direct cell-fate reprogramming enables self-assembly of dense neuronal niches

This paper describes a method to generate dense neuronal microcultures by combining fibroblast reprogramming with matrix remodeling in collagen microgels.

Benchmarking and Experimental Validation of Machine Learning Strategies for Enzyme Engineering

This study benchmarks machine learning models for enzyme engineering using a curated dataset, finding zero-shot predictors are most generalizable but still modestly correlated with activity, with consensus models improving precision.

Quanta microscopy enables fluorescence imaging at the native bit-plane rate

Quanta microscopy enables high-speed fluorescence imaging at the native bit-plane rate by reconstructing multibit images from sparse photon-detection maps.

Personalised prediction of anomalous bone regeneration in multiple myeloma patients

This study develops computational models to predict bone regeneration in multiple myeloma patients using patient data and genomics, showing rapid strengthening not fully explained by known mechanisms.

Megalin-mediated deterministic pseudotransduction in proximal tubules fundamentally challenges current understanding of AAV kidney transduction

The paper shows that AAV-mediated gene delivery to proximal tubules is not direct but relies on megalin-mediated uptake of reporter proteins from other organs, challenging current understanding.

Ultrasonic Detection of Venous Gas Emboli and Tissue Gas in Rodents: Toward Imaging Biomarkers for Decompression Stress

This study uses ultrasound to detect venous gas emboli and tissue gas in rats after decompression, finding a correlation between gas emboli severity and decompression risk.

Single-vector AAV delivery of En-ISPmu1 and donor DNA for in vivo targeted genome integration

The paper describes the development of En-ISPmu1, a modified TnpB nuclease, and demonstrates its ability to achieve targeted genome integration in mouse liver with a single AAV vector.

Three-Dimensional Fluid-Structure Interaction Reveals Trade-offs Among Oxygen Delivery, Shear, and Washout in Pediatric Roller-Pump Cardiopulmonary Bypass

This study uses a 3D fluid-structure interaction model to show that pediatric roller-pump settings with similar oxygen delivery can result in different shear and washout environments, suggesting the need for joint evaluation of these metrics.

Distinguishing matrix-associated mineralization from solution-driven calcium phosphate precipitation in osteogenic culture

The paper develops a kinetic model to distinguish between matrix-associated and solution-driven calcium phosphate mineralization in osteogenic cultures and shows that total mineral amount does not indicate matrix contribution.

In situ pulse dispersion estimation via third-harmonic generation interferometric autocorrelation for multiphoton microscopy

This paper develops a method to estimate pulse dispersion in multiphoton microscopy using third-harmonic generation interferometric autocorrelation, demonstrating its effectiveness in vivo.

Sequence-Independent In Situ Imaging of GlycoRNA in Living Cells and Tissues based on COMPASS

The paper introduces COMPASS, a method for sequence-independent imaging of glycoRNAs in living cells and tissues, revealing their distribution in cancer and neurogenesis.

Astrocyte modulation of neural activity during intracortical microstimulation

This study shows astrocytes actively shape neural activity during intracortical microstimulation by modulating calcium signaling, influencing neuronal responses in a pattern-dependent manner.

Directed Evolution of Multimodal Plastic Degrading Enzymes

This paper demonstrates the directed evolution of a multimodal plastic-degrading enzyme capable of breaking down both polyesters and polyurethane.

Growing Macroscopic Functional Human Tissues on 3D Printed Peptide Hydrogels

This study demonstrates growing macroscopic functional human tissues on 3D-printed peptide hydrogels, enabling centimeter-scale neural organoids and directional axonal communication.

Liquid-state surface fermentation as a platform for pure mycelium materials production

This study characterizes liquid-state surface fermentation (LSSF) for producing pure mycelium materials, identifying factors affecting biomass accumulation and glucose consumption.

Invisible shield: Sprayable supramolecular antimicrobial microscale films for preventing wound and medical device infections

This paper develops a sprayable antimicrobial film that prevents wound and medical device infections by forming a nanometric polyelectrolyte complex on surfaces.

From group-level neurophysiological biomarkers to individual-level phenotyping to identify mechanisms of neurodegenerative disease

This paper translates group-level neurophysiological biomarkers into individual-level phenotyping to identify mechanisms of neurodegenerative diseases using MEG data.

Erythrocyte aging links hypocalcemia to impaired flow dynamics and oxygen transport through volume dysregulation

This study shows that aging red blood cells rely on calcium for volume stability under shear stress, and impaired calcium regulation leads to flow and oxygen transport issues.

Low-Cost Real-Time Fiber-Optic Distance Sensing for OPM-based Biomagnetic Measurements

This paper evaluates low-cost polymer fiber sensors for real-time distance measurement in OPM-based biomagnetic measurements, finding high accuracy over 0-10 mm with some error beyond.

Hydrogel dimensionality directs mechanics-dependent nascent matrix deposition by tendon fibroblasts

This study shows that hydrogel dimensionality affects how tendon fibroblasts respond to mechanical cues, altering nascent matrix deposition.