A. Musacchio

Condensin II activation by M18BP1

The paper identifies M18BP1 as a factor required for condensin II localization to chromatin and shows it competes with MCPH1 to activate condensin II during mitosis.

Role of PLK1 in the epigenetic maintenance of centromeres

The paper identifies a phosphorylation network regulated by PLK1 that controls a conformational switch necessary for CENP-A deposition, essential for centromere maintenance.

Ipl1-controlled attachment maturation regulates Mps1 association with its kinetochore receptor

The paper shows that Ipl1/Aurora B kinase regulates Mps1's release from kinetochores by competing with Dam1 for binding to Ndc80:Nuf2, enabling proper chromosome segregation.

A mechanism that integrates microtubule motors of opposite polarity at the kinetochore corona

The paper identifies CENP-E's role in regulating DD kinetochore loading through the corona scaffold, ensuring coordinated bidirectional transport.

Stable kinetochore-microtubule attachment requires loop-dependent Ndc80-Ndc80 binding

This study shows that the Ndc80 loop facilitates Ndc80-Ndc80 interactions, which are essential for stable kinetochore-microtubule attachments and proper chromosome congression.

The Structural Flexibility of MAD1 Facilitates the Assembly of the Mitotic Checkpoint Complex

This study shows that structural flexibility in MAD1's hinge region is crucial for efficient MCC assembly and SAC signaling, with implications for understanding mitotic checkpoint function.

The Nucleocapsid Protein Of SARS-CoV-2, Combined With ODN-39M, Is A Potential Component For An Intranasal Bivalent Pancorona Vaccine

This paper evaluates a bivalent intranasal vaccine candidate combining SARS-CoV-2 N protein and ODN-39M, showing cross-reactive immunity against SARS-CoV-1 and enhanced Th1 response when combined with RBD.

Strain stiffening of Ndc80 complexes attached to microtubule plus ends

The paper measures strain stiffening of Ndc80 complexes under tension from depolymerizing microtubules, showing it is robust to mutations and linked to structural features and thermal fluctuations.

Conformational transitions of the mitotic adaptor Spindly underlie its interaction with Dynein and Dynactin

The paper shows Spindly adopts a closed conformation that blocks Dynein-Dynactin binding, and mutations induce an open state that enables interaction, with kinetochores promoting Spindly activation.

Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization

The paper reports a cryo-EM structure of the human CCAN complex and discusses its implications for CENP-A nucleosome recognition and centromere organization.

Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores

The paper reports the structure of the RZZ complex and demonstrates the molecular mechanism of corona assembly at human kinetochores using cryo-EM and in vitro assays.

JIP3 regulates bi-directional organelle transport in neurons through its interaction with dynein and kinesin-1

This study shows JIP3 interacts with dynein and kinesin-1 to regulate bi-directional organelle transport in neurons, preventing lysosome accumulation.

Reconstitution and use of highly active human CDK1:Cyclin-B:CKS1 complexes

The paper develops and compares protocols to produce and use highly active CDK1:Cyclin-B and CDK1:Cyclin-B:CKS1 complexes for mitotic protein machinery studies.

Assembly principles and stoichiometry of a complete human kinetochore module

This study reconstitutes a functional full-length CENP-C protein, demonstrating its role in kinetochore assembly and showing its stoichiometry matches endogenous human kinetochores.

Cyclin B1 scaffolds MAD1 at the corona to activate the spindle assembly checkpoint

This study identifies Cyclin B1 as a scaffold that localizes MAD1 to the kinetochore corona, enabling the spindle assembly checkpoint by making MAD1 phosphorylation independent of MPS1.

Human condensin I and II drive extensive ATP-dependent compaction of nucleosome-bound DNA

This study shows human condensin I and II compact nucleosome-bound DNA through ATP-dependent motor activity, forming reversible DNA loops without displacing nucleosomes.

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging, and chemical biology

This study demonstrates that electroporation can deliver recombinant proteins into human cells, maintaining their localization, function, and interactions, making it a versatile tool for chemical and synthetic biology.

Dynamic Kinetochore Size Regulation Promotes Microtubule Capture And Chromosome Biorientation In Mitosis

The paper shows that Spindly-based dynamic regulation of kinetochore size ensures proper microtubule capture and chromosome segregation by controlling corona expansion and shedding.

NDC80 clustering modulates microtubule dynamics under force

The paper shows that NDC80 clustering increases microtubule binding affinity and enables force-dependent modulation of kinetochore-microtubule attachments.