S. Raunser

Ideal efficacy photoswitches for TRPC4/5 channels harness high potency for spatiotemporally-resolved control of TRPC function in live tissues

This paper develops and tests ideal efficacy photoswitch ligands for TRPC4/5 channels, enabling precise spatiotemporally-controlled modulation in live tissues.

Multi-state kinetics of the syringe-like injection mechanism of Tc toxins

This study investigates the multi-state kinetics of Tc toxin prepore-to-pore transition, identifying intermediate states and transition times under various conditions.

Cryo-EM structure of Slo1 with the auxiliary γ1 subunit suggests mechanism of depolarization-independent activation

The paper presents a cryo-EM structure of Slo1 with the γ1 subunit, revealing how γ1 stabilizes the active conformation of the voltage-sensor domain and suggests a mechanism for depolarization-independent activation.

Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fate α

The paper identifies how spatial N-glycan rearrangements on α5β1 integrin nucleate galectin-3 oligomers, determining endocytic fate and retrograde trafficking.

Structure and activation mechanism of the Makes caterpillars floppy 1 toxin

This study determines the cryo-EM structure of Mcf1 toxin and elucidates its activation mechanism through conformational changes and autocleavage.

In situ structures from relaxed cardiac myofibrils reveal the organization of the muscle thick filament

This study reveals the 3D structure of relaxed cardiac sarcomeres using cryo-electron tomography, showing how thick filament components interact and function in muscle contraction.

Molecular mechanisms of inorganic-phosphate release from the core and barbed end of actin filaments

This study identifies a molecular backdoor mechanism for inorganic phosphate release from actin filaments, showing how a disease-linked mutation alters Pi release rates and filament structure.

Specialized pathogenic cells release Tc toxins using a type 10 secretion system

The paper identifies a type 10 secretion system in Yersinia entomophaga that releases Tc toxins via controlled cell lysis, revealing a novel lytic mechanism for toxin export.

TomoTwin: Generalized 3D Localization of Macromolecules in Cryo-electron Tomograms with Structural Data Mining

TomoTwin is a deep learning model for automated macromolecule localization in cryo-electron tomograms without manual training data, using deep metric learning to embed structures in a high-dimensional space.

Mechanism of threonine ADP-ribosylation of F-actin by a Tc toxin

This study reveals how TccC3 toxin modifies F-actin by ADP-ribosylating threonine-148, disrupting actin dynamics and causing cell death.

Structural basis of actin filament assembly and aging

This study presents cryo-EM structures of F-actin in all nucleotide states, revealing how water molecules drive ATP hydrolysis and filament dynamics, and explains differences in polymerization rates between Ca2+ and Mg2+-actin.

Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization

The paper reports a cryo-EM structure of the human CCAN complex and discusses its implications for CENP-A nucleosome recognition and centromere organization.

Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores

The paper reports the structure of the RZZ complex and demonstrates the molecular mechanism of corona assembly at human kinetochores using cryo-EM and in vitro assays.

Structure of the thin filament in native skeletal muscles reveals its interaction with nebulin and two distinct conformations of myosin

The paper presents the first structure of native nebulin bound to thin filaments in sarcomeres, revealing interactions with actin and myosin conformations, and the molecular basis of nebulin's role in muscle function.

Structure of a bacterial Rhs effector exported by the type VI secretion system

The paper presents the structure of the RhsA effector and its cognate VgrG1 spike protein from Pseudomonas protegens, revealing their assembly and potential mechanism of action via the T6SS.

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism

The paper reports high-resolution cryo-EM structures of actomyosin-V in three nucleotide states, revealing structural transitions and insights into force generation.

Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens

The paper investigates how actin interacts with nucleotidyl cyclase toxins from bacterial pathogens to activate them, revealing structural changes and allosteric mechanisms.

Structure of the human FERRY Rab5 effector complex

The paper determines the cryo-EM structure of the human FERRY Rab5 effector complex and shows Fy-2 acts as a binding hub for subunits and mRNA/Rab5.

Molecular architecture of black widow spider neurotoxins

This study reports cryoEM structures of two Latrotoxins (LaTXs) and proposes a cyclic oligomerization mechanism, revealing structural insights into their molecular action.

A structure-derived mechanism reveals how capping protein promotes nucleation in branched actin networks

This study reveals how capping protein (CP) promotes nucleation in branched actin networks by controlling access to terminal actin subunits and inhibiting nucleation promoting factors.

Structural basis for effector transmembrane domain recognition by type VI secretion system chaperones

The paper identifies a conserved motif in TMD-containing effectors and reveals how Eag chaperones stabilize these domains for T6SS-mediated secretion.

Molecular plasticity of the native mouse skeletal sarcomere revealed by cryo-ET

This study uses cryo-ET to reveal the molecular architecture of mouse skeletal sarcomeres, showing detailed interactions and conformational plasticity in actin and myosin filaments.

Structural basis of TRPC4 regulation by calmodulin and pharmacological agents

The paper reports structures of TRPC4 in complex with inhibitors, activators, and calmodulin, revealing how ligand binding and calmodulin interaction regulate the channel's conformation.

TranSPHIRE: Automated and feedback-optimized on-the-fly processing for cryo-EM

TranSPHIRE automates cryo-EM data processing during acquisition, using a feedback loop to improve particle picking and produce high-resolution 3D reconstructions.

Two particle picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER

This paper introduces two methods for selecting filamentous proteins from cryo-EM images: a deep learning approach in SPHIRE-crYOLO and a line detection approach in SPHIRE-STRIPER, showing their performance on tobacco mosaic virus and F-actin datasets.

Structural effects and functional implications of phalloidin and jasplakinolide binding to actin filaments

This study examines how phalloidin and jasplakinolide affect actin filament structure and function by stabilizing different states during actin dynamics.

Profilin and formin constitute a pacemaker system for robust actin filament growth

This study shows that profilin and formin work together to control actin filament growth rates, making them resilient to changes in soluble actin concentrations.

Towards the application of Tc toxins as a universal protein translocation system

The paper demonstrates that Tc toxins can be modified to deliver various small proteins across membranes using a combination of experimental techniques.

SPHIRE-crYOLO: A fast and accurate fully automated particle picker for cryo-EM

The paper introduces crYOLO, a deep learning-based particle picker for cryo-EM that achieves high accuracy and speed, enabling automated data pre-processing.

Electron cryo-microscopy structure of the canonical TRPC4 ion channel

The paper reports the cryo-EM structure of zebrafish TRPC4 in its closed, unliganded state, revealing key structural features and insights into its function and pharmacology.