Gennady Margolin

Elevated body temperature exacerbates arrhythmia and seizure-like activity in a zebrafish model of Timothy syndrome

This study shows that elevated temperature worsens arrhythmia and seizures in a zebrafish model of Timothy syndrome, suggesting fever may increase risk for TS patients.

Differential expression analysis in single cell and spatial RNASeq without model assumptions

The paper introduces a weighted averaging approach for differential expression analysis in single cell and spatial RNASeq to reduce false positives and negatives without assuming data distributions.

Specialized gas-exchange endothelium of the zebrafish gill

This study identifies and characterizes specialized endothelial cells in zebrafish gills that resemble mammalian lung aerocytes, using single-cell RNA sequencing and advanced imaging techniques.

Discovery and performance of DNA methylation panels for cancer detection and classification in blood

This paper identifies DNA methylation biomarker panels for multi-cancer detection and tissue-of-origin classification using blood samples, achieving high sensitivity and low false positive rates.

Parp1 deletion rescues cerebellar hypotrophy in xrcc1 mutant zebrafish

The paper shows that parp1 deletion rescues cerebellar hypotrophy in xrcc1 mutant zebrafish, suggesting PARP1 inhibition could be therapeutic for neurological disorders.

A novel transgenic reporter to study vertebrate epigenetics

The paper introduces an EpiTag zebrafish transgenic reporter to visualize epigenetic silencing/activation and identifies tissue-specific epigenetic regulatory genes through mutagenesis screening.

Early epigenetic priming of regeneration studied with a novel transgenic epigenetic reporter

The paper uses a novel transgenic reporter to study epigenetic reprogramming during zebrafish fin regeneration, identifying early chromatin remodeling events and key factors required for regeneration.

Anatomical and Molecular Characterization of the Zebrafish Meninges

This paper characterizes the zebrafish meninges using single-cell transcriptomics, identifying a novel cell type essential for brain development.

Mutations in the microexon splicing regulator srrm4 have minor phenotypic effects on zebrafish neural development

This study examines the role of srrm4 in zebrafish neural development and finds minimal phenotypic effects from mutations.

The transcriptional response of cortical neurons to concussion reveals divergent fates after injury

This study examines how cortical neurons respond to mild TBI, identifying ATF3 as a marker for stress-responsive neurons and showing that layer V neurons die while layer II/III neurons survive.

Profiling the endothelial translatome in vivo using 'AngioTag' zebrafish

The paper introduces a zebrafish transgenic line to profile endothelial cell translatomes in vivo, revealing novel genes and expression signatures across adult organs.

Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development

A 489,686-cell scRNA-seq atlas of 62 zebrafish stages (3.3–120 hpf) identifies 90 multi-tissue gene programs and markers for pericyte subtypes, intestinal smooth muscle layers, and best4+ enterocytes.

Improving the clinical performance of blood-based DNA methylation biomarkers utilizing locus-specific epigenetic heterogeneity

This paper develops EpiClass, a methylation density-based classifier that improves the performance of blood-based DNA methylation biomarkers in detecting ovarian cancer by leveraging locus-specific epigenetic heterogeneity.