L. D. Sibley

Bicyclic pyrrolidine inhibitors of Toxoplasma gondii phenylalanine t-RNA synthetase with antiparasitic potency in vitro and brain exposure

This paper describes new bicyclic pyrrolidine inhibitors of Toxoplasma gondii PheRS with improved potency, selectivity, and brain exposure compared to previous azetidine-based inhibitors.

Constitutive upregulation of transcription factors underlies permissive bradyzoite differentiation in a natural isolate of Toxoplasma gondii.

This paper identifies a Toxoplasma gondii isolate that constitutively expresses bradyzoite regulators and readily differentiates into mature bradyzoites under stress, providing a new in vitro model for studying chronic infection.

Variant Surface Protein GP60 Contributes to Host Infectivity of Cryptosporidium parvum

The paper investigates the role of GP60 in Cryptosporidium parvum infectivity, finding that while GP60 is not essential, its cleavage products affect parasite growth and infection severity.

Mendelian segregation and high recombination rates facilitate genetic analyses in Cryptosporidium parvum

This study investigates meiotic processes in Cryptosporidium parvum by analyzing chromosome segregation and recombination rates using transgenic parasites and in vitro cultures, revealing Mendelian inheritance and high crossover rates.

SKSR1 identified as key virulence factor in Cryptosporidium by genetic crossing

The study identifies SKSR1 as a key virulence factor in Cryptosporidium by crossing isolates with different virulence and analyzing genetic loci associated with infectivity.

A high-fidelity in vitro model of the human intestinal epithelium

This paper develops an in vitro model of the human intestinal epithelium that closely mimics in vivo cell types using single-cell transcriptomics and a biomedical foundation model.

A Combination of Four Nuclear Targeted Effectors Protects Toxoplasma Against Interferon Gamma Driven Human Host Cell Death During Acute Infection.

This study identifies four nuclear-targeted effectors in Toxoplasma gondii that block IFN-gamma signaling and prevent host cell death during acute infection.

Cryptosporidium infection of human small intestinal epithelial cells induces type III interferon and impairs infectivity of Rotavirus.

This study develops a human air-liquid interface culture system for Cryptosporidium hominis, revealing it induces type III interferon and reduces Rotavirus infectivity.

Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum

This study identifies multiple pathways for glucose phosphate transport and utilization in Cryptosporidium parvum, revealing alternative mechanisms for obtaining phosphorylated hexose.

Microbiota produced indole metabolites disrupt host cell mitochondrial energy production and inhibit Cryptosporidium parvum growth

The paper identifies indole metabolites from the microbiota that inhibit Cryptosporidium parvum growth by disrupting host mitochondrial function and parasite mitosome membrane potential.

Molecular Basis for Interferon-mediated Pathogen Restriction in Human Cells

This study identifies RNF213 as essential for IFN-gamma-mediated restriction of intracellular pathogens like Toxoplasma gondii and Mycobacterium tuberculosis through ubiquitination, independent of autophagy.

Over-expression Screen of Interferon-Stimulated Genes Identifies RARRES3 as a Restrictor of Toxoplasma gondii Infection

The paper screens interferon-stimulated genes to identify RARRES3 as a factor restricting Toxoplasma gondii infection by inducing premature parasite egress.

Intrinsically disordered pathogen effector alters the STAT1 dimer to prevent recruitment of co-transcriptional activators CBP/p300

This study identifies how the parasite Toxoplasma gondii uses the disordered protein TgIST to block STAT1-mediated gene expression by altering STAT1 dimer conformation and preventing activator recruitment.

Toxoplasma bradyzoites exhibit physiological plasticity of calcium and energy stores controlling motility and egress.

This study shows that Toxoplasma bradyzoites have reduced calcium and energy stores, limiting their motility and egress, but they can rapidly restore these stores upon release from cysts.

Insulinase like protease 1 contributes to macrogamont formation in Cryptosporidium parvum

This study identifies INS1 as a protease critical for macrogamont formation and oocyst production in Cryptosporidium parvum, with loss of INS1 reducing virulence in mice.

Gut metabolites influence susceptibility of neonatal mice to cryptosporidiosis.

This study identifies gut metabolites that influence susceptibility to Cryptosporidium infection in neonatal mice, showing that fatty acids affect parasite growth and invasion.

Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis

This study identifies a Toxoplasma gondii effector that inhibits necroptosis by modulating host repressor complexes, thereby preventing cell death and promoting chronic infection.

Evolution of resistance in vitro reveals a novel mechanism of artemisinin activity in Toxoplasma gondii.

This study identifies novel mutations in Toxoplasma gondii that confer resistance to artemisinin, including mitochondrial genome amplification and changes in mitochondrial function.

Toxoplasma gondii infection drives conversion of NK Cells into ILC1s

This study shows Toxoplasma gondii infection converts NK cells into ILC1-like cells that persist and circulate independently of infection.

Evaluation of current and emerging anti-malarial medicines for inhibition of Toxoplasma gondii growth in vitro

This study evaluates anti-malarial drugs for their ability to inhibit Toxoplasma gondii growth in vitro, finding limited effectiveness for most compounds.