K. Wiehe

Engineering immunogens that select for specific mutations in HIV broadly neutralizing antibodies

This study uses molecular dynamics simulations to design HIV-1 immunogens that select for specific mutations in broadly neutralizing antibodies.

Germline-targeting SOSIP trimer immunization elicits precursor CD4 binding-site targeting broadly neutralizing antibodies in infant macaques.

This study shows that a multi-dose immunization with germline-targeting SOSIP vaccines induces precursor broadly neutralizing antibodies in infant macaques, similar to adult responses.

Computing the inducibility of B cell lineages under a context-dependent model of affinity maturation: Applications to sequential vaccine design

The paper develops a computational model to estimate the inducibility of specific antibodies during vaccine design by simulating context-dependent mutation rates in B cell lineages.

Vaccine induction of CD4-mimicking broadly neutralizing antibody precursors in macaques

This study shows that vaccinating macaques with a CD4bs-targeting immunogen elicits neutralizing bnAb precursors with structural features of CD4-mimicking antibodies.

Engineered Immunogens to Elicit Antibodies Against Conserved Coronavirus Epitopes

This study designs engineered immunogens targeting conserved coronavirus epitopes to elicit broad-spectrum antibodies, showing protection in mice against live virus challenge.

Mutation-Guided Vaccine Design: A Strategy for Developing Boosting Immunogens for HIV Broadly Neutralizing Antibody Induction

The paper demonstrates a mutation-guided vaccine strategy that induces HIV broadly neutralizing antibodies in mice by selecting for rare B cell lineage mutations.

Structural basis for breadth development in a HIV-1 neutralizing antibody

This paper determines structures of HIV-1 antibodies at different stages of maturation to understand how neutralization breadth develops through affinity optimization.

Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike

This study determines cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike and compares them to BA.1, revealing structural changes and immune evasion mechanisms.

Structural diversity of the SARS-CoV-2 Omicron spike

The paper compares cryo-EM structures of SARS-CoV-2 Omicron and Delta spikes to analyze conformational changes affecting immune evasion and transmissibility.

A single, improbable B cell receptor mutation confers potent neutralization against cytomegalovirus

Identification of CDRH3 Loops in the Naive B Cell Receptor Repertoire that Can Be Engaged by Candidate Immunogens

The paper identifies CDRH3 loops in the naive B cell receptor repertoire that can be engaged by candidate immunogens using a combined experimental and computational approach.

Different adjuvanted pediatric HIV envelope vaccines induced distinct plasma antibody responses despite similar B cell receptor repertoires in infant rhesus macaques.

Ability of nucleoside-modified mRNA to encode HIV-1 envelope trimer nanoparticles

This study shows that nucleoside-modified mRNA can encode HIV-1 Env trimer nanoparticles that induce bnAb precursor B cell expansion and neutralizing activity in mice.

Augmenting Neutralization breadth against Diverse HIV-1 by increasing the Ab-Ag interface on V2

This study identifies a lineage of antibodies from infected rhesus macaques that neutralize 54% of HIV-1 strains by expanding the antibody-Env interface around the V2 loop.

A broadly neutralizing antibody protects against SARS-CoV, pre-emergent bat CoVs, and SARS-CoV-2 variants in mice

A broadly neutralizing antibody protects against multiple SARS-CoV and SARS-CoV-2 variants in mice by targeting a conserved epitope in the receptor-binding domain.

SARS-CoV-2 vaccination induces neutralizing antibodies against pandemic and pre-emergent SARS-related coronaviruses in monkeys

This study shows that SARS-CoV-2 vaccines induce neutralizing antibodies against SARS-CoV-1, SARS-CoV-2, and batCoVs in monkeys.

Rapid selection of HIV envelopes that bind to neutralizing antibody B cell lineage members with functional improbable mutations

The paper identifies a minimal set of mutations required for the function of a HIV bnAb and demonstrates in vitro selection of envelope immunogens that can bind to antibodies with these mutations.

The functions of SARS-CoV-2 neutralizing and infection-enhancing antibodies in vitro and in mice and nonhuman primates

This study examines SARS-CoV-2 neutralizing and infection-enhancing antibodies, showing in vitro enhancement does not always lead to in vivo disease enhancement, but can cause increased lung inflammation in monkeys.

Mouse and Human Antibodies that Bind HLA-E-Leader Peptide Complexes and Enhance NK Cell Cytotoxicity

The paper isolates antibodies that bind HLA-E-Leader peptide complexes and enhance NK cell cytotoxicity in both mice and humans.

Recapitulation of HIV-1 Env-Antibody Coevolution in Macaques Leading to Neutralization Breadth

Virus-antibody coevolution in rhesus macaques recapitulates developmental features of human antibodies.

Fab-dimerized glycan-reactive antibodies neutralize HIV and are prevalent in humans and rhesus macaques

The paper identifies Fab-dimerized glycan-reactive antibodies in HIV-infected macaques and HIV-naïve humans that neutralize HIV-1 and can be elicited by vaccination.

Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements

This study identifies a critical role for W571 in HIV-1 Env allostery by showing mutations disrupting CD4-induced rearrangements and rendering Env insensitive to CD4 binding.

Antibody feedback limits the expansion of cognate memory B cells but drives the diversification of vaccine-induced antibody responses

The paper shows antibody feedback limits memory B cell expansion but allows diversification of vaccine-induced antibody responses by suppressing dominant epitopes while enabling subdominant ones.

Functional Improbable Antibody Mutations Critical for HIV Broadly Neutralizing Antibody Development

The paper identifies improbable mutations critical for HIV broadly neutralizing antibody development and proposes a mutation-guided vaccine strategy.