R. J. Harding

Huntingtin is an RNA-binding protein and participates in NEAT1-mediated paraspeckles

This study identifies huntingtin as an RNA-binding protein that interacts with G-rich RNA sequences, including those in the NEAT1 paraspeckle lncRNA.

Small molecule screen identifies non-catalytic USP3 chemical handle

The paper identifies a small molecule ligand for the ZnF-UBD of USP3 with a KD of 14 µM, which could serve as a starting point for developing DUBTACs.

Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6

The paper identifies and characterizes a chemical probe, SGC-UBD253, that targets the HDAC6 UBD with selectivity, and a less active derivative as a negative control.

Expanding the Huntingtons disease research toolbox; validated huntingtin subdomain constructs for biochemical and structural investigation of the huntingtin protein

This paper develops validated HTT subdomain constructs for biochemical and structural studies of huntingtin protein interactions and function.

Structure Activity Relationship of USP5 Allosteric Inhibitors

This paper develops a chemical series of USP5 allosteric inhibitors, identifies a selective compound with structural and functional characterization.

HAP40 orchestrates huntingtin structure for differential interaction with polyglutamine expanded exon 1

This study reveals how HAP40 orchestrates HTT structure, affecting its interaction with polyglutamine-expanded exon 1, providing insights into Huntington's disease mechanisms.

Discovery of small molecule antagonists of the USP5 zinc finger ubiquitin-binding domain

The paper identifies small molecule antagonists targeting the USP5 ZnF-UBD, providing a framework for developing chemical probes to study its role in ubiquitin signaling.

Design and characterisation of mutant and wild-type huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems

This paper develops a toolkit for producing mutant and wild-type huntingtin proteins in eukaryotic systems to enable biochemical and structural studies of HD-related mutations.