V. Nizet

An efficient in vivo-inducible CRISPR interference system for group A Streptococcus genetic analysis and pathogenesis studies

This paper develops a doxycycline-inducible CRISPRi system for GAS to enable efficient gene repression and presents a genome browser for GAS genetic features.

Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

This study shows that Tamm-Horsfall protein enhances neutrophil NETosis during UTI by modulating respiratory chain, granules, and chromatin remodeling pathways.

Perinatal ampicillin administration modulates murine bile acid metabolism in vivo - an observational study

This study examines how ampicillin exposure via different routes affects bile acid metabolism in mice, finding significant changes in maternal bile acids and increased infant weight.

Glycan-specific IgM is critical for human immunity to Staphylococcus aureus

This study identifies WTA-specific IgM as a critical immune factor for human protection against S. aureus, outperforming IgG in opsonophagocytic activity and correlating with clinical outcomes.

A conserved antigen induces respiratory Th17-mediated serotype-independent protection against pneumococcal superinfection

The paper identifies a conserved antigen, LafB, that provides serotype-independent protection against pneumococcal superinfection via Th17 cells, independent of antibodies.

Unrecognized Potent Activities of Colistin Against Clinically Important mcr+ Enterobacteriaceae Revealed in Synergy with Host Immunity

This study reveals that colistin has previously unrecognized bactericidal activity against mcr-1+ Gram-negative bacteria in physiologic conditions and synergizes with host immunity.

Elucidation of independently modulated genes in Streptococcus pyogenes reveals carbon sources that control its expression of hemolytic toxins

The paper identifies carbon sources that regulate hemolytic toxin expression in Streptococcus pyogenes using transcriptome-wide independent component analysis.

Strengthening of enterococcal biofilms by Esp

This study investigates the role of Esp in strengthening enterococcal biofilms under acidic conditions, showing that Esp unfolding and aggregation enhance biofilm stability.

Coordination of CcpA and CodY regulators in Staphylococcus aureus USA300 strains

This paper uses ICA to modularize the TRN of S. aureus USA300 and combines it with a metabolic model to show coordination between CcpA and CodY in regulating carbon and amino acid metabolism.

An M protein coiled coil unfurls and exposes its hydrophobic core to capture LL-37

The paper reveals how M87 protein from Streptococcus pyogenes interacts with LL-37 by unfolding its coiled coil to expose a hydrophobic core, neutralizing the antimicrobial peptide.

Design of a Streptococcus pyogenes M protein immunogen to elicit M type cross-reactivity

The paper designs an M protein immunogen that elicits cross-reactivity against multiple M types by targeting a conserved 3D pattern in the hypervariable region.

Vascular proteome responses precede organ dysfunction in sepsis

This study identifies vascular proteome changes preceding organ dysfunction in sepsis using a murine model and integrative analysis.

Human milk oligosaccharides reduce murine group B Streptococcus vaginal colonization with minimal impact on the vaginal microbiota

This study shows that human milk oligosaccharides reduce GBS vaginal colonization in mice without significantly altering the vaginal microbiota.

Repurposed Drugs Block Toxin-Driven Platelet Clearance by the Hepatic Ashwell-Morell Receptor to Clear Staphylococcus aureus Bacteremia

CCL28 modulates neutrophil responses during infection with mucosal pathogens

This study shows CCL28 enhances neutrophil responses in mucosal infections, improving pathogen clearance in some cases but worsening disease in others.

TLR4 signaling and macrophage inflammatory responses are dampened by GIV/Girdin

This study shows GIV/Girdin dampens TLR4-mediated inflammatory responses in macrophages by inhibiting receptor dimerization and downstream signaling.

Revealing 29 sets of independently modulated genes in Staphylococcus aureus, their regulators and role in key physiological responses

This paper identifies 29 independently modulated gene sets in S. aureus using ICA, linking them to regulators and physiological responses.

Environmental conditions dictate differential evolution of vancomycin resistance in Staphylococcus aureus

This study identifies media-specific mutational mechanisms of vancomycin resistance in S. aureus using adaptive laboratory evolution and sequencing.

Characterizing the response of Acinetobacter baumannii ATCC 17978 to azithromycin in multiple in vitro growth conditions

This study examines how MDR A. baumannii responds to azithromycin in different growth conditions using multiple analytical techniques.

Role of Inflammasome-independent Activation of IL-1β by the Pseudomonas aeruginosa Protease LasB

This study identifies that the Pseudomonas aeruginosa protease LasB directly activates IL-1β independently of inflammasomes, contributing to lung inflammation and pathology during infection.

Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes

This study identifies prophage-encoded exotoxins in Streptococcus pyogenes that enhance colonization fitness and pathogenesis in scarlet fever.

Identifying the effect of vancomycin on HA-MRSA strains using bacteriological and physiological media

The paper examines vancomycin's effect on HA-MRSA USA100 strains using bacteriological and physiological media through growth curves, RNA sequencing, and exo-metabolomics.

Decomposition of transcriptional responses provides insights into differential antibiotic susceptibility

The paper investigates how media composition affects antibiotic susceptibility in E. coli using transcriptomics and machine learning, identifying key regulators and shifts in respiration and iron availability.

Profiling the effect of nafcillin on HA-MRSA D592 using bacteriological and physiological media

This study investigates how nafcillin affects HA-MRSA D592 in different media types, revealing media-dependent antibiotic activity through multi-dimensional analysis.

Exploration of bacterial bottlenecks and Streptococcus pneumoniae pathogenesis by CRISPRi-seq

This study uses CRISPRi-seq to identify genetic factors important for Streptococcus pneumoniae pneumonia, revealing a critical bottleneck at 48 h post-infection and gene fitness differences between in vitro and in vivo conditions.

Chemical Impacts of the Microbiome Across Scales Reveal Novel Conjugated Bile Acids

The paper identifies novel conjugated bile acids produced by the microbiome in mice and humans using mass spectrometry and a murine 3D model.

PHLPP1 Counter-regulates STAT1-mediated Inflammatory Signaling

This paper identifies PHLPP1 as a phosphatase that dephosphorylates STAT1 to regulate inflammatory signaling in macrophages, with loss of PHLPP1 protecting mice from sepsis.

Streptococcal Lancefield polysaccharides are critical cell wall determinants for human group IIA secreted phospholipase A2 to exert its bactericidal effects

This study identifies Lancefield polysaccharides as critical for hGIIA to kill Group A and B Streptococcus by enabling penetration through the bacterial cell wall.

Evidence that recurrent Group A streptococcus tonsillitis is animmunosusceptibility disease involving antibody deficiency and aberrant Tfh cells

The paper investigates recurrent Group A Streptococcus tonsillitis and finds it involves antibody deficiency and aberrant Tfh cells, with genetic factors contributing to susceptibility.