William M. Shih

A computational framework for designing micron-scale crisscross DNA megastructures

This paper develops a computational framework to design crisscross DNA megastructures, optimizing handle assignment and expanding handle libraries to enable large-scale fabrication.

Single-Molecule Imaging Reveals Differential Stability of Alpha-Synuclein Aggregates

This study uses single-molecule imaging to show that Parkinson's disease-associated alpha-synuclein aggregates are more stable than physiological multimers under dilution.

Enhanced lung delivery of an immunostimulatory duplex RNA augments the antitumor activity by reshaping systemic cytokine pharmacodynamics

This study shows that lung-targeted delivery of immunostimulatory dsRNA via LNPs enhances systemic cytokine responses and antitumor activity in melanoma models.

Validation of DoriVac (DNA origami vaccine) efficacy in a metastatic melanoma model

This study validates DoriVac, a DNA origami vaccine, as an effective treatment for metastatic melanoma in mouse models, showing reduced tumor nodules and enhanced immune activation when combined with PD-L1 blockade.

Single-molecule Mechanostructural Fingerprinting of Nucleic Acid Conformations

The paper introduces a DNA nanoswitch calipers platform to simultaneously measure structural and mechanical properties of biomolecules, demonstrating its application on telomeric DNA G-quadruplexes.

Reusing excess staple oligonucleotides for economical production of DNA origami

This paper demonstrates that reusing excess staple oligonucleotides with replenishment can reduce DNA origami production costs by up to 80%.

Cargo quantification of functionalized DNA origami for therapeutic application

The paper quantifies cargo on DNA origami nanoparticles using gel-based and fluorescence-based methods for therapeutic applications.

Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccination

This study developed a DNA origami-based cancer vaccine that co-delivers antigen and CpG immune adjuvant with an optimal nanospacing for Th1 immune polarization.

Multi-micron crisscross structures from combinatorially assembled DNA-origami slats

Crisscross polymerization of DNA-origami slats enables the creation of micron-scale structures with uniquely addressable nanoscale features.

Robust nucleation control via crisscross polymerization of DNA slats

This paper introduces a method for robust nucleation control in DNA-based self-assembly using crisscross polymerization, achieving strictly seed-initiated growth without spontaneous nucleation.

A smart polymer for sequence-selective binding, pulldown and release of DNA targets

This paper introduces a methanol-responsive polymer (MeRPy) that selectively binds and releases DNA targets, improving NGS library quality by depleting unwanted transcripts.

Cell-free transcriptional regulation via nucleic-acid-based transcription factors

The paper demonstrates the use of nucleic-acid-based transcription factors to implement complex transcriptional logic in cell-free systems.

Rapid and scalable in vitro production of single-stranded DNA

This paper describes a method for producing high-purity single-stranded DNA for various applications.