Jan J. Molenaar

A microscopy-based readout to assess tumour-specific viability in neuroblastoma co-cultures and short-term cultured patient samples

This paper introduces a microscopy-based method to measure tumour-specific viability in neuroblastoma co-cultures and patient samples, enabling more accurate drug response assessment.

Metastatic tumor cells in bone marrow differ from paired neuroblastoma tumor and contain subsets with therapy-resistant characteristics

Sensitivity profiling reveals consistent drug responses across preclinical neuroblastoma models

This study compares ex vivo short-term, organoid, and PDX drug screening methods in neuroblastoma, finding ex vivo screens are faster and more consistent with in vivo results.

The ITCC-P4 PDX platform of pediatric cancers for preclinical testing

The paper introduces a PDX platform for pediatric cancers, providing in vivo drug testing models and molecular data to aid in therapy development.

Proteomic profiling of the neuroblastoma secretome identifies extracellular vesicles as drivers of T cell suppression

The paper identifies extracellular vesicles as immunosuppressive factors in neuroblastoma by profiling secretomes and showing their ability to suppress T cell activation.

γδ T cells are the prime anti-tumoral T cells in pediatric neuroblastoma

The paper shows that γδ T cells, not β T cells, are the main anti-tumoral immune cells in pediatric neuroblastoma after chemotherapy.

The immune landscape of pediatric Brain and Solid Tumors: A systematic review and integration of immunohistochemistry and single-cell RNA sequencing data

This study integrates immunohistochemistry and single-cell RNA sequencing data to characterize the immune landscape of pediatric brain and solid tumors, revealing distinct immune microenvironment features across tumor types.

Blocking MIF secretion enhances CAR T-cell efficacy against neuroblastoma

This study identifies MIF as a key immunosuppressive factor in neuroblastoma that inhibits CAR T-cell efficacy, showing that blocking MIF enhances T-cell activation and therapeutic potential.

Effective methods for bulk RNA-Seq deconvolution using scnRNA-Seq transcriptomes

The paper evaluates deconvolution methods for bulk RNA-Seq using scnRNA-Seq data, identifies biases, and introduces SQUID, a method that outperforms others in predicting cell mixtures and identifying cancer subclones.

Systematic discovery of gene fusions in pediatric cancer by integrating RNA-seq and WGS

This paper develops a method to detect tumor-specific gene fusions in pediatric cancer by integrating RNA-seq and WGS, identifying 165 high-confidence fusions and their structural variants.