Sirisha Mukkavalli

DNA-PK enforces the S/G2 checkpoint in ATM-deficient cells to limit ATR inhibitor efficacy

This study shows DNA-PK enforces the S/G2 checkpoint in ATM-deficient cells, reducing ATR inhibitor efficacy, and suggests combining ATR and DNA-PK inhibition could enhance treatment in such cancers.

Defective Microhomology-Mediated End-joining in SMARCB1-Deficient Tumors

This study identifies a defect in MMEJ repair in SMARCB1-deficient tumors, leading to hyper-dependence on the FA/BRCA pathway, which can be targeted for therapy.

The p97 adaptor p47/NSFL1C is necessary for stress granule dissolution after heat stress

This study shows that p47 is required for p97 recruitment to stress granules, which is necessary for their disassembly after heat stress.

Disruption of Microhomology-mediated End-joining in Ewing Sarcoma

This paper shows that Ewing sarcoma cells have reduced POLθ expression and MMEJ activity due to EWS-FLI1 or EWSR1 disruption, making them sensitive to inhibitors of FA, NHEJ, or HR pathways.

USP1 inhibition promotes RAD18-dependent PCNA degradation and BRCA1 synthetic lethality

This paper identifies a PIP motif in RAD18 essential for PCNA ubiquitination and shows USP1 inhibition leads to synthetic lethality in BRCA1-deficient cells.

CHAMP1 Complex Promotes Heterochromatin Assembly and Reduces Replication Stress

This study identifies the CHAMP1 complex as a regulator of heterochromatin assembly and replication fork stability, showing its role in preventing genomic instability in cancer cells.

HPV16 E6 oncoprotein promotes microhomology-mediated viral integration by upregulating POLQ expression

This study shows HPV16 E6 oncoprotein increases POLQ levels via UBE3A and RAD23A regulation, enhancing MMEJ to promote viral integration and oncogenesis.

CHAMP1 complex directs heterochromatin assembly and promotes homology-directed DNA repair

This study identifies the CHAMP1 complex's role in heterochromatin assembly and its enhancement of homology-directed DNA repair in specific genomic regions.